The Covaris® M220 microTUBE Prep Station enables the easy loading of sample into snap-cap and screw-cap microTUBEs. Each package contains 1 preparation station.
Size: 1 each
The Covaris® M220 miniTUBE Accessory Kit is ideally suited for Ion PGM™ users requiring large DNA fragments for their applications (mate-paired libraries, etc), providing tight DNA fragment distributions centered on 2,000, 3,000 or 5,000 bp. The kit includes a holder, 25 clear miniTUBEs for 2 kb fragments, 25 blue miniTUBEs for 3 kb fragments, and 25 red miniTUBEs for 5 kb fragments. The kit also includes a preparation station for easy loading and unloading of sample into the miniTUBEs.
Size: 1 kit
The Covaris® M220 miniTUBE Holder is required to process the sample tube in the M220 Focused-ultrasonicator™. This holder is compatible with clear, blue, and red miniTUBEs. Each package contains 1 holder station.
Size: 1 kit
The Covaris® M220 miniTUBE Prep Station enables the easy loading of sample into clear, blue, or red miniTUBEs. A tilted stand is included that has been designed to help recover the entire sample from the miniTUBE. Each package contains 1 preparation station.
Size: 1 each
This software module for the QuantStudio™ 12K Flex Real-Time PCR System supports compliance with 21 CFR Part 11 FDA guidelines on electronic records security and prevents unauthorized access to the instrument. When loaded on the system, this software will: • Authenticate each user’s log-in information • Lock out access and require re-authentication if user is inactive for a set period of time • Log any unauthorized attempts to access the system software in the Event Log • Verify each user’s permission to perform predefined Controlled Activities • Permits E-signature function for data verificat...
Size: 1 kit
This software module expands the functionality of the QuantStudio™ 12K Flex software, allowing high resolution melting (HRM) analysis to be performed on PCR amplicons. High Resolution Melt (HRM) analysis is a new, post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. Simple and fast, this method is based on PCR melt (dissociation) curve techniques and is enabled by the recent availability of improved double-stranded DNA (dsDNA)-binding dyes along with next-generation real-time PCR instrumentation and analysis software. HRM analysis can discriminate DNA sequ...
Size: 1 kit
QuantStudio™ 12K Flex Software supports the QuantStudio™ 12K Flex Real-Time PCR System. The software allows the user to open and analyze experiments generated from the QuantStudio™ 12K Flex Real-Time PCR System. The software also enables the user to set up experiments, send experiments to the instrument, control the thermal cycling process in the instrument, collect data and analyze the collected data, all in an integrated and streamlined fashion. This configuration includes a license kit with 10 keys for 10 users. >> Download the software installer (a license key is required for use) Features...
Size: 10 seats
mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo , because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m 7 G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its structure precludes its incorporation at any other position in the RNA molecule. How mMESSAGE mMACHINE® kits work mMESSAGE mMACHINE® kits have a simplified reaction for...
Size: 25 reactions w⁄ manual
The LIVE/DEAD® Fixable Blue Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer. • Stable—dyes are freeze dried in separate vials to maintain stability • Robust—staining pattern is the same before and after fixation • Bright signal—allows for easy distinction between live/dead cells in single channel Stable Unlike products that are so...
Size: 1 kit
The 5500 W Barcode Conversion Primers 1-16 Kit contains barcoded primers for libraries constructed with truncated adaptors, such as Agilent Technologies' SureSelect™ libraries, for multiplex sequencing on 5500 W Series genetic analyzers. This kit does not contain 5500 W Conversion Enzyme and should be used in conjunction with the 5500 W Conversion Primers Kit . This kit contains primers for 16 barcodes.
Size: 48 reactions
The Ion PI™ Template OT2 200 Kit v2 contains template preparation reagents for automated and reproducible template preparation using the Ion OneTouch™ 2 System for Ion Proton™ System semiconductor sequencing. As an integral component of the Ion Proton™ semiconductor sequencing workflow, the Ion PI™ Template OT2 200 Kit v2 is designed to make high-quality templated Ion PI™ Ion Sphere™ particles using the Ion OneTouch™ 2 System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion PI™ Template OT2 20...
Size: 8 reactions
The CellROX® Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX® Deep Red Reagent, as well as SYTOX® Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS). The CellROX® Deep Red Flow Cytometry Assay Kit features: • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines,...
Size: 100 assays
Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) Antibody is labeled with our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize cross-reactivity, it has been adsorbed against mouse IgG, mouse serum, and human serum prior to conjugation.
Size: 0.5 mL
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 495/518 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples Get Superior Results in Your Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Flu...
Size: 300 units
Applied Biosystems® Oligo (dT) 16 is a homo-oligomeric deoxyribonucleotide (poly dT) used for priming and reverse transcription of polyadenylated (poly A+) mRNA. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
Size: 1 kit
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Size: 20 reactions
The amine-reactive Lissamine™ rhodamine B sulfonyl chloride can be used to create red-fluorescent bioconjugates with excitation/emission maxima ~568/583 nm.
Size: 1 g
Alexa Fluor® 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor® 647 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 650/668 nm Superior to antibody staining Optimal for fixed and permeabilized samples Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has...
Size: 300 units
Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K + /H + ionophore nigericin , which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing concentration of extracellular K + .
Size: 10 mg