Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) Antibody is labeled with our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. It is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of immunoglobulin light chains from rat. To minimize cross-reactivity, it has been adsorbed against mouse IgG, mouse serum, and human serum prior to conjugation.
Size: 0.5 mL
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli , insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have att R sites for recombination with any att L-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available. Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, an...
Size: 6 µg
Applied Biosystems® Oligo (dT) 16 is a homo-oligomeric deoxyribonucleotide (poly dT) used for priming and reverse transcription of polyadenylated (poly A+) mRNA. Note : See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
Size: 1 kit
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Size: 20 reactions
This Applied Biosystems® product is designed for the purification of RNA from whole blood for real-time RT-PCR, microarrays, or other high-quality RNA experiments. 12 samples can be processed in about 45 minutes. For use with the ABI PRISM® 6100 Nucleic Acid PrepStation, sufficient reagents are included for 24 purifications. • Extract pure, intact RNA • Isolate more than 6 µg of RNA from 3 mL blood • Simple workflow—less time and fewer steps than other kits (see Figure) • Highly stable RNA, free of contaminating gDNA, and inhibitors; no DNase treatment required • Purified RNA used for the gene...
Size: 24 preps
The Midi Gel Adapter is designed for use with the Novex® Midi Gel Cassette (NuPAGE® or Tris-Glycine Gels) to perform electrophoresis using a BioRad Criterion™ Cell. The Midi Gel Adapter is also supplied with the Novex® Midi Gels with Adapters Kit.
The highly deionized (Hi-Di) Formamide is used to resuspend samples before electrokinetic injection in capillary electrophoresis systems. The smaller configuration (5 ml) minimizes the number of freeze⁄thaw cycles compared to the larger 25ml bottle and may be a better fit when running a lower number of samples. For Research Use Only. Not for use in diagnostics procedures.
Size: 4 x 5 mL
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Endogenous Control Plate contains 32 genes, plated in triplicate, that have been shown to be good candidates for such an experiment. This collection of genes has been selected from literature searches and⁄or whole genome microarray tests carried ...
Size: 1 x 96-well plate
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features: •Cytomegalovirus (CMV) promoter for high-level expression • att R sites for Gateway® cloning, enabling recombination with att L-flanked fragments •C-terminal V5 tag for easy detection •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli Gateway® Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination ve...
Size: 6 µg
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool . This RPMI is modified as follows: With Without • L-glutamine • HEPES • Phenol Red
Size: 10 x 500 mL
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with Invitrogen's fluorochrome-conjugated monoclonal antibodies.
Size: 500 mL
Ambion® Silencer ® KIF11 (Eg5) Positive Control siRNA (Human, Mouse, Rat) is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls • Functionally tested in several common cell lines • HPLC purified, duplexed, and ready to use Silencer ® control siRNAs are unmodified 21-mers; they are designed to be used with Silencer ® siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs....
Size: 5 nmol
Applied Biosystems® GeneAmp® Thin-Walled Reaction Tubes enable fast heat transfer for precise PCR in 0.5 mL PCR Systems. • Thin plastic enables fast heating and cooling for accurate results • Flat, frosted caps for easy marking • Captive lid with positive-click closure assures proper seating • Polished surface and conical bottom allow maximum sample recovery • Autoclaved tubes provide a clean, controlled start Rapid Heat Transfer The GeneAmp® Reaction Tube is made of specially engineered polypropylene and is optimized for use with the Veriti™ 60-Well Thermal Cycler and the Dual 96-Well GeneAmp...
Size: 1000 tubes
These are the same Ambion® primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 µM and are functionally tested using the RETROscript® Kit.
Size: 80 µL
Recombinant Mouse Fibroblast Growth Factor Basic (also known as FGF Basic, bFGF, FGF 2, FGF-β or Heparin Binding Growth Factor) is a bioactive protein intended for use in cell culture applications. FGF-basic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. • High purity —no interference from other proteins or contaminants • High biological activity —more results with less...
Size: 100 µg
mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo , because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m 7 G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its structure precludes its incorporation at any other position in the RNA molecule. How mMESSAGE mMACHINE® kits work mMESSAGE mMACHINE® kits have a simplified reaction for...
Size: 25 reactions w⁄ manual
Ambion® Acid Phenol:Chloroform:IAA (25:24:1) is premixed and supplied at pH 6.6 (pH 7.9 ± 0.2), with included buffer. Acid Phenol:Chloroform:IAA is provided in one bottle of 100 mL. In RNA and DNA extraction procedures, Acid Phenol:Chloroform:IAA (pH 7.9) helps to stabilize the interface and prevents foaming when mixing. Preparation of phenol for use in molecular biology applications is a time-consuming and often hazardous procedure due to its toxic and corrosive nature. Ambion® premixed, quality tested, saturated phenols are ready-to-use, eliminating handling problems and offering a more conv...
Size: 100 mL
The Magnetic 96-Well Separator is designed for fast and simple removal of supernatant from samples bound to magnetic particles and may be used manually or as an integrated part of an automated workstation. The Magnetic 96-Well Separator is an ideal tool for use in singleplex and multiplex biological assays based on the Luminex® xMAP® Technology. These assays make use of Luminex® MagPlex™, or magnetic microspheres, and the MAGPIX®, Luminex® 200™ or FLEXMAP 3D® instruments. Note: It is recommended that the Magnetic 96-Well Separator be used with a standard 96-well flat-bottom plate such as Grein...
Size: 1 each
• The Heat Equalizing Block is an aluminum conduction plate for use with the SSP UniTray and other laboratory procedures that require even heat distribution during thermal cycling. The Heat Equalizing Block is set on top of the SSP UniTray while thermal cycling to ensure even heat distribution • The Membrane Comb Loading Tray is for convenient benchtop loading of 64-lane membrane combs. Polycarbonate tray with two rows of 64 wells, each 2 mm wide • HLA Beyond Tears - By Glenn E. Rodey, Second Edition (De Novo, Inc., Durango, CO 2000) Serves as an introductory text for individuals who are inter...
Size: 1 each
The SuperScript® III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A) + or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product th...
Size: 50 reactions