The Champion™ pET Expression System yields the highest-level protein production in E. coli . During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli , which significantly improves ...
Size: 20 reactions
The Ion PI™ Template OT2 200 Kit v2 contains template preparation reagents for automated and reproducible template preparation using the Ion OneTouch™ 2 System for Ion Proton™ System semiconductor sequencing. As an integral component of the Ion Proton™ semiconductor sequencing workflow, the Ion PI™ Template OT2 200 Kit v2 is designed to make high-quality templated Ion PI™ Ion Sphere™ particles using the Ion OneTouch™ 2 System to streamline the overall Ion sequencing workflow, yield significant time savings, increase productivity, and accelerate scientific discovery. The Ion PI™ Template OT2 20...
Size: 8 reactions
The Covaris® M220 miniTUBE Accessory Kit is ideally suited for Ion PGM™ users requiring large DNA fragments for their applications (mate-paired libraries, etc), providing tight DNA fragment distributions centered on 2,000, 3,000 or 5,000 bp. The kit includes a holder, 25 clear miniTUBEs for 2 kb fragments, 25 blue miniTUBEs for 3 kb fragments, and 25 red miniTUBEs for 5 kb fragments. The kit also includes a preparation station for easy loading and unloading of sample into the miniTUBEs.
Size: 1 kit
The 5500 W Barcode Conversion Primers 1-16 Kit contains barcoded primers for libraries constructed with truncated adaptors, such as Agilent Technologies' SureSelect™ libraries, for multiplex sequencing on 5500 W Series genetic analyzers. This kit does not contain 5500 W Conversion Enzyme and should be used in conjunction with the 5500 W Conversion Primers Kit . This kit contains primers for 16 barcodes.
Size: 48 reactions
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein. In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to...
Size: 20 reactions