The pYES2-DEST52 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent expression in Saccharomyces cerevisiae. As part of the YES™ Vector Collection, pYES2-DEST52 carries the promoter and enhancer sequences from the GAL1 gene for regulated expression in S.cerevisiae. pYES2-DEST52 features:
• 2μ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal medium • C-terminal V5 epitope and polyhistidine(6xHis) tags for efficient detection and purification tR sites for efficient recombination with any attL-flanked Gateway™ entry vector
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
GAL1
Product Type
Gateway System Destination Expression Vector
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
Gateway
Vector
pYES, pDEST
Product Line
Gateway™
Contents & Storage
The pYES2-DEST52 Gateway™ destination vector is provided supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.