TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

We offer a variety of TrypLE formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco TrypLE Express is:

Gentle on cells

• Room-temperature stable
• Easy to use
• Animal origin-free (AOF)


The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.

Gentle On Cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.

Room-Temperature Stable
TrypLE Express is room-temperature stable and ready to use. TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to Use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin-Free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco TrypLE Express at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.