The GeneAmp™ Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti™ 96-Well Thermal Cycler. Features of this master mix:
• Set up reactions quickly, using premixed reagents • Detect a single copy of a gene in as little as 10 ng of DNA • Get reproducible, specific, and sensitive results
Easy to use Pre-mixed, hot-start chemistries enable you to set up your experiments quickly. And by generating greater than 90% “A” overhangs—it makes cloning easier too.
Detect a single copy in 10 ng of DNA Use the GeneAmp™ Fast PCR Master Mix to detect a single copy of a gene in 10 ng of human genomic DNA or as little as 10 copies of an HIV target.
Part of the Applied Biosystems™ Fast PCR System The Applied Biosystems™ Fast PCR System, consisting of the premixed GeneAmp™ Fast PCR Master Mix, specially designed microplates and tubes, and the Veriti™ 96-Well Thermal Cycler (0.1 mL or 0.2 mL), considerably reduces PCR reaction times.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Primer-probe
Format
Tube
GC-Rich PCR Performance
Medium
Polymerase
AmpliTaq DNA Polymerase
Reaction Speed
Fast
Exonuclease Activity
5' - 3'
Product Line
GeneAmp™
Product Type
Fast PCR Master Mix
Quantity
250 Reactions
Shipping Condition
Dry Ice
Sufficient For
125 Reactions
Concentration
2X
Fidelity (vs. Taq)
1 X
Hot Start
Built-In Hot Start
No. of Reactions
250 Reactions
Overhang
3'-A
Reaction Format
SuperMix or Master Mix
Size (Final Product)
2 kb or less
Starting Material
DNA
Volume
0.1 mL or 0.2 mL
Contents & Storage
The GeneAmp™ Fast PCR Master Mix (2X) contains two tubes (1.25 mL each) sufficient for 125 20μl PCR reactions. The Fast PCR Master Mix can also be ordered with a protocol.