Megaplex™ Primer Pools, Human Pools A v2.1 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex PreAmp Primers, Human Pools A v2.1. Megaplex™ RT Primers, Human Pool A v2.1 contains RT primers for 377 unique microRNAs and 4 controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool B. Human Pool A is intended for use with the TaqMan® Array Human MicroRNA A Card v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.