TaqMan® Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode
Product Line
TaqMan™
For Use With (Application)
Real Time PCR (qPCR), Stem Cell Analysis
Detection Method
Primer-Probe Detection
Label or Dye
FAM
Product Type
Protein Assays Core Reagents Base Kit
Sample Type
Tissue, Cells
Hot Start
No
No. of Reactions
500
Passive Reference Dye
ROX (Pre-mixed)
Polymerase
AmpliTaq™ Fast DNA Polymerase
Primer Probe Compatibility
TaqMan™
PCR Method
qPCR Protein Quantification
Reaction Speed
Fast
Shipping Condition
Wet Ice
Target Specificity
Not Target-Specific
Contents & Storage
The TaqMan™ Protein Assays Core Reagents Base Kit contains: