Applied Biosystems™

SYBR™ Select Master Mix for CFX

Catalog number: 4472954
Applied Biosystems™

SYBR™ Select Master Mix for CFX

Catalog number: 4472954
Catalog Number
4472954
Unit Size
10 x 5 mL
Price (USD)
Price: 4,180.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1 x 1 mL
Price (USD)
Price: 118.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1 x 5 mL
Price (USD)
Price: 491.00
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-
Quantity
Catalog Number
Unit Size
2 x 5 mL
Price (USD)
Price: 944.00
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-
Quantity
Catalog Number
Unit Size
5 x 5 mL
Price (USD)
Price: 2,185.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
447295410 x 5 mL
Price: 4,180.00
Your price:
-
44729371 x 1 mL
Price: 118.00
Your price:
-
44729421 x 5 mL
Price: 491.00
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-
44729522 x 5 mL
Price: 944.00
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44729535 x 5 mL
Price: 2,185.00
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-
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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix for CFX and added additional capabilities for your gene expression analysis.

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SYBR™ Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity
SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR™ GreenER™ Dye and Heat-labile UDG
• SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

View performance data.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
SYBR
Form
Liquid
Format
Vial
GC-Rich PCR Performance
High
PCR Method
qPCR
Polymerase
AmpliTaq DNA Polymerase
Reaction Speed
Fast or Standard
For Use With (Equipment)
AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Label or Dye
SYBR GreenER
Passive Reference Dye
ROX (Pre-mixed)
Product Line
SYBR™
Product Type
Real Time PCR SYBR Master Mix
Purity or Quality Grade
UP (Ultra Pure)
Quantity
10 x 5 mL
Sufficient For
5000 Reactions of 20 μL
For Use With (Application)
Gene Expression
Concentration
2X
Hot Start
Built-In Hot Start
No. of Reactions
5000 Reactions
Sample Type
DNA (Genomic), cDNA
Volume
10 x 5 mL

Contents & Storage

The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 10 X 5 mL bottles, sufficient for 5000 reactions at 20 μL. Store at 2-8™C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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