The Applied Biosystems™ TaqMan™ GAPDH Assay with JUN™ dye-labeled QSY™ probe provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. The assay consists of a JUN™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers, and it can be used for multiplex or singleplex PCR reactions.

Benefits of this assay include:

• Eliminate assay design time by using pre-designed primers and pre-designed JUN™ dye-labeled TaqMan™ probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays

Save time in assay design, formulation, and testing by using the TaqMan™ GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

The JUN™ dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN™ dye) due to an overlap of spectra. TaqMan™ Multiplex Master Mix with MUSTANG PURPLE™ dye is recommended.