Qdot™ 625 ITK™ carboxyl quantum dots are the ideal starting material for preparing custom conjugates that require high loading of biomolecules. These materials are carboxylate functionalized and can be coupled to amine groups of proteins and modified oligonucleotides using EDC-mediated condensation. The coatings of these probes provides more binding sites than our Qdot™ ITK™ amino quantum dots, but lacks PEG linkers that help to prevent non-specific interactions. These materials can be conjugated to X-PEG-amine bi-functional linkers for custom reactivity and higher specificity. Our Qdot™ ITK™ carboxyl quantum dots are provided as 8 μM solutions and are available in all 9 Qdot™ probe colors.

Important Features of Qdot™ ITK™ Carboxyl Quantum Dots:
• Qdot™ 625 ITK™ carboxyl quantum dot has emission maxima of ∼625 nm
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for various labeling and tracking applications

Properties of Qdot™ Nanocrystals
Qdot™ probes are ideal for imaging and labeling applications that require bright fluorescent signals and/or real-time tracking. Unique among fluorescent reagents, all nine available colors of Qdot™ probes can be simultaneously excited with a single (UV to blue-green) light source. This property makes these reagents excellent for economical and user-friendly multiplexing applications. Qdot™ labels are based on semiconductor nanotechnology and are similar in scale to moderately sized proteins.

About the Innovator’s Tool Kit Qdot™ ITK™ Reagents
These Qdot™ ITK™ probes are ideal for researchers who wish to prepare specific (non-stocked) conjugates for their applications and need customizable conjugation functionality.

Other Forms of Qdot™ Nanocrystals are Available
In addition to the carboxyl-derivatized form, we offer Qdot™ ITK™ quantum dots with amino and aliphatic hydrocarbon modifications. We’ve also developed a wide range of Qdot™ nanocrystals conjugates and labeling kits. Investigate the properties of Qdot™ nanocrystals or read the Molecular Probes™ Handbook Section 6.6—Qdot™ Nanocrystals to find out more.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.