The SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip® workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperScript® II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix’ protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Biotin-based
Format
Kit
Label or Dye
Biotin
Final Product Type
cDNA (Double-stranded)
Sample Type
Poly(A+) RNA, Total RNA
Labeling Target
cDNA
For Use With (Application)
For generating double-stranded cDNA
Includes Label or Dye
No
For Use With (Equipment)
Affymetrix GeneChip™
Labeling Method
Direct Labeling
No. of Reactions
30
Product Line
SuperScript™
Product Type
cDNA Kit
Quantity
30 Reactions
Shipping Condition
Dry Ice
Contents & Storage
50 μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water All components are guaranteed stable for 6 months when properly stored.