Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Fluorescent
For Use With (Application)
Cell Viability and Proliferation
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Product Type
Carboxy-DFFDA SE
Dye Type
Oregon Green™ 488
Emission
488
Form
Solid
Product Line
CellTrace™, Oregon Green™
Reagent Type
Cell Tracker Compounds, Cell Labeling Reagents
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)
Contents & Storage
Contains 20 vials of Oregon Green™ 488 carboxylic acid diacetate, SE (50 μg/vial). Store in freezer (-5 to -30°C) and protect from light.