Recombinant Human TGF beta 1 (TGFB1) is a bioactive protein intended for use in cell culture applications. TGFB1 acts synergistically with TGF-α in inducing transformation and acts as a negative autocrine growth factor. Dysregulation of TGFB1 activation and signaling may result in apoptosis. The lyophilized TGFB1 Recombinant Human is verified for activity through dose-dependent inhibition of proliferation of mouse HT-2 cells stimulated with mouse IL-4.
• Highly Pure—minimizes interference from other proteins or contaminants • Active—get more results with less protein
High Purity To help verify that GIBCO™ Recombinant growth factors are of the highest quality, each protein is analyzed for purity, along with structural homogeneity, to help ensure a biologically active protein. The purity of each GIBCO™ recombinant growth factor is verified by SDS-PAGE to be over 95% pure and to have endotoxin levels below 0.1 ng/μg.
High Biological Activity All GIBCO™ Recombinant growth factors are verified for activity. Each lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our TGFB1 Recombinant Human growth factor is determined by the dose-dependent inhibition of proliferation of mouse HT-2 cells stimulated with mouse IL-4 to have an ED50 ≤0.2ng/mL. Optimal concentration for individual applications should be determined by a dose response assay.