The synthetic steroid hormone dexamethasone binds to the glucocorticoid receptor, producing a steroid-receptor complex that then localizes in the nucleus and regulates gene transcription. The green fluorescent analog, fluorescein dexamethasone should be useful for studying the mechanism of glucocorticoid receptor activation, although at a slower rate than unmodified dexamethasone.

For stock concentrations up to 1 mM, DMF or aqueous buffers above pH 6 are recommended as diluent. Stock solutions can be stored as single-use aliquots, protected from light at -20°C.