Smad cell signaling is a critical pathway involved in cell growth and proliferation. The CellSensor SBE-bla HEK 293T cell line is responsive to transforming growth factor-beta 1 (TGF-ß1) and can be used to probe the Smad signaling pathway.The CellSensor SBE-bla HEK 293T cell line contains the ß-lactamase reporter gene under the control of the Smad Binding Element (SBE). The SBE-bla construct was transduced into HEK 293T cells by lentivirus to obtain the cell line. Flow cytometry was used to isolate a clone responsive to TGF-ß1 a dose response curve for this cell line using TGF-B1 is available in the validation packet data. Academic and non-profit customers, please inquire for special pricing.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Product Line
CellSensor™
Target Entry
TGF beta/BMP pathway, TGF beta R
Type
Cell Line
Cell Line
HEK 293T
System Type
CellSensor™
Druggable Target
Signaling Pathway
Contents & Storage
CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.