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Invitrogen™
Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1R E. coli
Catalog number: K282020
Invitrogen™
Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1R E. coli
Catalog number: K282020
Catalog Number
K282020
also known as K2820-20
Unit Size
25 reactions
Price (USD)
Price: 758.00
Your price:
Availability
-
Quantity
Catalog Number
K282040
also known as K2820-40
Unit Size
50 reactions
Price (USD)
Price: 1,330.00
Your price:
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| K282020 also known as K2820-20 | 25 reactions | Price: 758.00 Your price: | - | ||
| K282040 also known as K2820-40 | 50 reactions | Price: 1,330.00 Your price: | - |
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Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO™ Vector for 5-minute benchtop ligations without ligase (1). The pCR™-Blunt II-TOPO™ Vector also features:
• ccdB gene for positive selection (2)
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
• SP6 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening
Selected Zero Blunt™ TOPO™ PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
• ccdB gene for positive selection (2)
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and Zeocin™ resistance genes for your choice of selection in E. coli
• SP6 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening
Selected Zero Blunt™ TOPO™ PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bacterial or Yeast Strain
DH5α
Cloning Method
Blunt TOPO™
Format
Kit
Product Line
One Shot™
Product Type
PCR Cloning Kit
Promoter
SP6
Quantity
25 Reactions
Vector
Blunt DNA Cloning Vectors
Contents & Storage
Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid
Store at -68 to -85°C.
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid
Store at -68 to -85°C.
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