The Novex™ AP Mouse Chemiluminescent Detection Kit provides superior chemiluminescent detection of proteins transferred to either nitrocellulose or PVDF membranes and treated with a protein-specific primary antibody. Detection is performed by treatment of the membrane with an alkaline phosphatase (AP)-conjugated anti-mouse secondary antibody followed by a ready-to-use chemiluminescent CDP-Star™ substrate of alkaline phosphatase. Protein-specific signal is then captured by a chemiluminescent-compatible imaging system or X-ray film.
The Novex™ AP Mouse Chemiluminescent Detection Kit is compatible with most western protocols but was designed for use with the iBind™ Western System.
Each kit includes a secondary AP-conjugated anti-mouse antibody, CDP-Star™ chemiluminescent substrate, and an enhancer, sufficient for 10 mini-membranes processed using the iBind™ Western System.
Features include:
• Easy-to-use protocol • High specificity, clean background • Ultra-sensitivity • Long-lasting signals—up to 5 days • Results in less 5 minutes
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Chemiluminescent
Format
Kit
For Use With (Application)
Western Blotting
Product Line
Novex™
Species
Mouse
Substrate
CDP-Star AP substrate
Sufficient For
10 Mini Membranes
For Use With (Equipment)
iBind™
Includes
Secondary AP-conjugated anti-mouse antibody, CDP-Star™ chemiluminescent substrate, and an enhancer