The pcDNA™6⁄TR vector is part of the T-REx™ System (catalog K102001), which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known (1,2).
The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor (TR) protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Inducible
Delivery Type
Transfection
Promoter
CMV
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Blasticidin
Shipping Condition
Room Temperature
Protein Tag
Untagged
Vector
pcDNA
Product Line
T-REx™, pcDNA™
For Use With (Application)
Regulated Expression
Contents & Storage
20 μg of supercoiled vector (40 μL at 0.5 μg/μL) supplied in TE Buffer, pH 8.0. Store at -20°C.