PML/RARA Fusion Transcripts Panel

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  • Citations (2)

Research Focus

Promyelocytic leukemia (APL or AML-M3) is a subtype of acute myeloid leukemia with distinct clinical and histopathologic features.Genetically, APL is characterized by a unique chromosomal anomaly. APL accounts for 10–15% of all AML cases. Investigations suggest that 99% of APL patients harbor a translocation between chromosomes 15 and 17, which fuses the retinoic acid receptor alpha (RARA) gene on chromosome 17 with the PML gene on chromosome 15. Detection of the PML/RARA t(15;17) translocation is therefore used within clinical research as an identifyer for APL. The PML/RARA t(15;17) translocation is used in conjunction with the investigation of therapy response to all-trans-retinoic acid and arsenic trioxide as well as research on therapy response monitoring via MRD and research regarding detection of early relapse. Depending on the location of breakpoints within the PML site, intron 6, exon 6 and intron 3, the respective PML-RARa transcript subtypes referred to as long (L or bcr1), variant (V or bcr2) and short (S or bcr3), may be formed. They represent 55%, 5% and 40% of the cases respectively.

Target Disease/Application

Acute Promyelocytic Leukemia (APL)

Chromosomal Alteration

t(15;17)(q21;q22)

Assay ID Clinical Name Gene Symbol Gene Aliases Amplicon Length  
Hs03043651_ft PMLex6/RAR ex3L-form (=BCR1) PML-RARA PML RARA 77
Hs03024794_ft PMLex3/RAR ex3S-form (=BCR3) PML-RARA PML RARA 104

Specifications

General Specifications

# of assays: 1
Technology: TaqMan® Assays - Pre-formulated assay (20x or 60x mix)
Scale: Available in multiple scales
Intended Use: Research Use Only

Kit Storage

-20° C

Documents

Product Literature

 

Citations & References (2)

  • Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program.

Authors
Gabert J et al.
Journal
Leukemia. 2003 Dec;17(12):2318-57.
  • Characterization of Cryptic Rearrangements and Variant Translocations in Acute Promyelocytic Leukemia

Authors
David Grimwade et al.
Journal
Blood, Vol 90, No 12 (December 15), 1997: pp 4876-4885