AKT1 (total) Human ELISA Kit

The AKT1 (Total) Human ELISA kit is designed to detect and quantify the level of AKT1 protein. This assay is intended for the detection of AKT1 from human cell lysates using 96-well plates and a microplate reader. ...

Specifications

Marker
Akt1
Gene ID
207
Species
Human
Platform
Microplate Reader
Gene Symbol
AKT1
Sensitivity
0.1 ng/mL
Target Gene
v-akt murine thymoma viral oncogene homolog 1
Gene Aliases
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
0.3-30 ng⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NM_001014431.1
NP_001014432.1
NP_005154.2
NM_005163.2
NM_001014432.1
NP_001014431.1
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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AKT1 [pS473] Human Ultrasensitive ELISA Kit

The AKT [pS473] Human Ultrasensitive ELISA research use only kit is designed to detect and quantify the level of AKT protein phosphorylated at serine residue 473. This assay is intended for the detection of AKT [pS473] Ultrasensitive from human, mouse, or rat cell lysates using 9 ...

Specifications

Marker
Akt1
Gene ID
207
Species
Human
Platform
Microplate Reader
Gene Symbol
AKT1
Sensitivity
0.1 U⁄mL
Target Gene
v-akt murine thymoma viral oncogene homolog 1
Gene Aliases
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
0.3-25 U⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NM_001014431.1
NP_001014432.1
NP_005154.2
NM_005163.2
NM_001014432.1
NP_001014431.1
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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AKT1 (PKB alpha) Recombinant Human Protein

AKT1 (PKB alpha) is a serine⁄threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. ...

Specifications

Form
Frozen
Species
Human
Gene Aliases
AKT1 (PKB alpha)
Kinase Group
Ser⁄Thr Kinases: ACG Group
Accession Number
NP_005154
Molecular Weight
59.1 kDa
Research Category
Drug Development
Validated Application
Kinase Assay
Protein Tag or Fusion
His Tag
Expression
Baculovirus
Kinase Family
AKT Kinase Family
Protein Form
Full Length
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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AKT [pS473] mAb (clone 99H9L9), ABfinity™ recombinant

AKT [pS473] ABfinity™ Recombinant Rabbit Monoclonal Antibody - Purified and tested with human samples in sandwich ELISAs. AKT [pS473] is product of AKT1 gene with gene ID of 207 also known as v-akt murine thymoma viral oncogene homolog 1, AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RA ...

Specifications

Reactivity
Human
Validated Application
ELISA
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
99H9L9
Antibody Fragment
Whole Antibody
Target Gene
AKT [pS473]
Target Molecule
Phosphoproteins
Gene ID
207
Gene Symbol
AKT1
Accession Number
P31749
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Affinity Purified
Concentration
0.5 mg⁄ml
Color
Unconjugated
Form
Frozen
Neutralizing Antibody
No
Protein Family
Kinases & Inhibitors
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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PRAS40 [pT246] Rabbit Polyclonal Antibody, Alexa Fluor® 488 Conjugate

PRAS40 [pT246) ALEXA CONJ; Alexa Fluor 488 conjugated Polyclonal antibody specific to Human, (Mouse) PRAS40 [pT246]. This antibody is validated for use in Immunocytochemistry/ Immunofluorescence. Anti-PRAS40 [pT246] recognizes the expressed product of the AKT1S1 gene. ...

Specifications

Reactivity
Human
Mouse
Validated Application
Immunohistochemistry
Host
Rabbit
Clonality
Polyclonal
Target Gene
PRAS40 [pT246]
Gene ID
84335
Label or Dye
Alexa Fluor® 488
Excitation⁄Emission (nm)
488⁄519
Target Modification State
Phospho
Purification
Antiserum
Research Category
Apoptosis
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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AKT [pT308] oAb (clone B18HCLC), ABfinity™ recombinant

This purified oligoclonal recombinant rabbit IgG antibody recognizes AKT [pT308] in human samples. ...

Specifications

Reactivity
Human
Validated Application
ELISA
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Clone ID
B18HCLC
Antibody Fragment
Whole Antibody
Target Gene
AKT [pT308]
Gene ID
11651
Gene Symbol
Akt1
Accession Number
P31749
Label or Dye
Unconjugated
Purification
Purified
Concentration
0.5 mg⁄ml
Form
Liquid
Neutralizing Antibody
No
Protein Family
Kinases & Inhibitors
Comments
Oligoclonal (Pool of Monoclonal Abs to Several Epitopes)
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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AKT [pS473] mAb (clone 98H9L8), ABfinity™ recombinant

This purified ABfinity™ recombinant rabbit anti-AKT [pS473] monoclonal antibody reacts with human and mouse AKT1 [pS473]. AKT1 [pS473] is encoded by the 207 gene, also known as RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, protein kinase B (PKB). Anti-AKT [pS473] monoc ...

Specifications

Reactivity
Human
Mouse
Validated Application
ELISA
Western Blot
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
98H9L8
Antibody Fragment
Whole Antibody
Target Gene
AKT [pS473]
Gene ID
207
Accession Number
P31749
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Affinity Purified
Concentration
0.5 mg⁄ml
Color
Unconjugated
Form
Liquid
Neutralizing Antibody
No
Platform
Fluorescence Microscope
Confocal Microscope
Flow Cytometer
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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PRAS40 [pT246] Oligoclonal Antibody (clone 8HCLC), ABfinity™ Recombinant

This purified oligoclonal recombinant rabbit IgG antibody recognizes Proline Rich Akt Substrate of 40 kDa (PRAS40 [pT246]) in human samples. ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Immunofluorescence
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Clone ID
8HCLC
Antibody Fragment
Whole Antibody
Gene ID
84335
Accession Number
Q96B36
Label or Dye
Unconjugated
Concentration
0.5 mg⁄ml
Form
Liquid
Neutralizing Antibody
No
Comments
Oligoclonal (Pool of Monoclonal Abs to Several Epitopes)
Research Category
Apoptosis
Signal Transduction
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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PRAS40 Monoclonal Antibody (clone 4H14L8), ABfinity™ Recombinant

This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes Proline Rich Akt Substrate of 40 kDa (PRAS40) in human samples. ...

Specifications

Reactivity
Human
Validated Application
Immunofluorescence
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
4H14L8
Antibody Fragment
Whole Antibody
Gene ID
84335
Accession Number
Q96B36
Label or Dye
Unconjugated
Concentration
0.5 mg⁄ml
Form
Liquid
Neutralizing Antibody
No
Research Category
Apoptosis
Signal Transduction
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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PRAS40 Mouse Monoclonal Antibody (clone 73P21)

This mouse anti-PRAS40 monoclonal antibody is specific to human PRAS40. PRAS40 monoclonal antibody recognizes the expressed product of the AKT1S1 gene. PRAS40 is a 40kDa substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. Val ...

Specifications

Reactivity
Human
Validated Application
Western Blot
Immunoprecipitation
Host
Mouse
Clonality
Monoclonal
Clone ID
73P21
Target Gene
PRAS40
Gene ID
84335
Label or Dye
Unconjugated
Research Category
Apoptosis
Signal Transduction
Other Additives
Sodium Azide Preservative
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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AKT Mouse mAb (clone 9Q7)

This purified mouse anti-AKT monoclonal antibody is specific to human, mouse and rat AKT. AKT monoclonal antibody recognizes the expressed product of the AKT1 gene. AKT, also known as Protein Kinase B (PKB) or RAC-α, is a serine/threonine kinase that plays an important role in mu ...

Specifications

Reactivity
Human
Rat
Mouse
Validated Application
Western Blot
Host
Mouse
Clonality
Monoclonal
Clone ID
9Q7
Target Gene
Akt
Gene ID
10000, 207, 208
Label or Dye
Unconjugated
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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AKT [pS473] Antibody Pair

This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of phosphorylated Akt [pS473]. Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use buffers and solutions optimized for use with Invitrogen Antibod ...

Specifications

Form
Concentrated
Marker
Akt [pS473]
Gene ID
207
Platform
Microplate Reader
Reactivity
Human
Gene Symbol
AKT1
Target Gene
Akt [pS473]
Gene Aliases
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Label or Dye
HRP (Horseradish Peroxidase)
Emission Class
Visible
Protein Family
Kinases & Inhibitors
Target Molecule
Proteins (Phosphoproteins)
Accession Number
NM_001014431.1
NP_001014432.1
NP_005154.2
NM_005163.2
NM_001014432.1
NP_001014431.1
Research Category
Signal Transduction
Validated Application
ELISA
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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LanthaScreen® Akt HEK 293E Cell Line

LanthaScreen® GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. The PI3K⁄AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. Because of t ...

Specifications

System
LanthaScreen®
Readout
TR-FRET
Reporter
GFP (EmGFP)
Cell Line
293E (HEK)
Cell State
Dividing Cells
Antagonists
LY294002
PI-103
Gene Symbol
AKT1
Primary Agonist
IGF-1
Detection Method
Fluorescent
Druggable Target
Signaling Pathway
Kinases
Optimal Response
IGF-1 (EC50 = 0.42 µg/ml)
Alternate Agonists
Insulin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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PRAS40 (total) Mouse ELISA Kit

The PRAS40 (Total) Mouse ELISA kit is designed to detect and quantify the level of PRAS40 protein. This assay is intended for the detection of PRAS40 from mouse, human and rat cell lysates using 96-well plates and a microplate reader. ...

Specifications

Marker
PRAS40
Gene ID
84335, 292887
Species
Rat
Human
Mouse
Platform
Microplate Reader
Gene Symbol
Akt1s1
AKT1S1
Sensitivity
<200 pg⁄mL
Target Gene
AKT1 substrate 1 (proline-rich)
Gene Aliases
PRAS40, Lobe, MGC2865, PRAS40
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
0.313-20 ng⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Adaptor Proteins
Cross-Reactivity
Human
Rat
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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