Genomics and proteomics search results

AKT1 (PKB alpha) Recombinant Human Protein (Life Technologies™)

AKT1 (PKB alpha) is a serine⁄threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. ...

Specifications

Form
Frozen
Species
Human
Gene Aliases
AKT1 (PKB alpha)
Kinase Group
Ser⁄Thr Kinases: ACG Group
Accession Number
NP_005154
Molecular Weight
59.1 kDa
Research Category
Drug Development
Validated Application
Kinase Assay
Protein Tag or Fusion
His Tag
Expression
Baculovirus
Kinase Family
AKT Kinase Family
Protein Form
Full Length
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV3599
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AKT1 (total) Human ELISA Kit (Novex®)

The AKT1 (Total) Human ELISA kit is designed to detect and quantify the level of AKT1 protein. This assay is intended for the detection of AKT1 from human cell lysates using 96-well plates and a microplate reader. ...

Specifications

Marker
Akt1
Gene ID
207
Species
Human
Platform
Microplate Reader
Gene Symbol
AKT1
Sensitivity
0.1 ng/mL
Target Gene
v-akt murine thymoma viral oncogene homolog 1
Gene Aliases
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
0.3-30 ng⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NM_001014431.1
NM_005163.2
NP_001014432.1
NM_001014432.1
NP_001014431.1
NP_005154.2
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

KHO0531
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AKT1 [pS473] Human Ultrasensitive ELISA Kit (Novex®)

The AKT [pS473] Human Ultrasensitive ELISA research use only kit is designed to detect and quantify the level of AKT protein phosphorylated at serine residue 473. This assay is intended for the detection of AKT [pS473] Ultrasensitive from human, mouse, or rat cell lysates using 9 ...

Specifications

Marker
Akt1
Gene ID
207
Species
Human
Platform
Microplate Reader
Gene Symbol
AKT1
Sensitivity
0.1 U⁄mL
Target Gene
v-akt murine thymoma viral oncogene homolog 1
Gene Aliases
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
0.3-25 U⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NM_001014431.1
NM_005163.2
NP_001014432.1
NM_001014432.1
NP_001014431.1
NP_005154.2
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

KHO0541
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AKT [pS473] Monoclonal Antibody (clone 99H9L9), ABfinity™ Recombinant (Novex®)

AKT [pS473] ABfinity™ Recombinant Rabbit Monoclonal Antibody has been validated for use in applications such as Western Blot, Immunofluorescence and Flow Cytometry. AKT [pS473] is product of AKT1 gene with gene ID of 207 also known as v-akt murine thymoma viral oncogene homolog 1 ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Western Blot
Immunofluorescence
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
99H9L9
Antibody Fragment
Whole Antibody
Target Gene
AKT [pS473]
Target Molecule
Proteins (Phosphoproteins)
Gene ID
207
Gene Symbol
AKT1
Accession Number
P31749
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Affinity Purified
Concentration
0.5 mg⁄ml
Color
Unconjugated
Form
Frozen
Neutralizing Antibody
No
Protein Family
Kinases & Inhibitors
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

700256
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AKT (Pan) Rabbit pAb (Novex®)

This mouse anti-AKT (Pan) polyclonal antibody is specific to human and mouse Akt and expected to react with rat Akt (not tested). AKT (Pan) polyclonal antibody recognizes the expressed product of the AKT1 gene also known as AKT2, AKT3. Akt, also known as Protein Kinase B (PKB) or ...

Specifications

Reactivity
Mouse
Human
Validated Application
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
Akt (pan)
Gene ID
10000, 207, 208
Label or Dye
Unconjugated
Research Category
Signal Transduction
Other Additives
Sodium Azide Preservative
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44609G
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PRAS40 [pT246] Polyclonal Antibody, Rabbit (Novex®)

This purified, unconjugated Rabbit anti-PRAS40 [pT246] polyclonal antibody is specific to human and mouse PRAS40 [pT246]. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. Rabbit anti-PRAS40 [pT246] polyclonal antibody is us ...

Specifications

Reactivity
Mouse
Human
Validated Application
Western Blot
Immunohistochemistry
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
PRAS40 [pT246]
Gene ID
84335, 67605
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Purified
Research Category
Apoptosis
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

441100G
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AKT/PKB [pS473] mAb, Rabbit (14-6) (Novex®)

The purified rabbit AKT/PKB [pS473] monoclonal antibody is specific to human, mouse, and rat Akt [pS473]. AKT/PKB plays an important role in regulation of metabolism, cell survival and growth. Western blotting and immunocytochemistry are the validated applications for AKT/PKB [pS ...

Specifications

Reactivity
Human
Mouse
Validated Application
Western Blot
Immunohistochemistry
Host
Rabbit
Clonality
Monoclonal
Clone ID
14-6
Target Gene
Akt/PKB [pS473]
Gene ID
207, 24105
Label or Dye
Unconjugated
Target Modification State
Phospho
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44621G
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PRAS40 Mouse Monoclonal Antibody (clone 73P21) (Novex®)

This mouse anti-PRAS40 monoclonal antibody is specific to human PRAS40. PRAS40 monoclonal antibody recognizes the expressed product of the AKT1S1 gene. PRAS40 is a 40kDa substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. Val ...

Specifications

Reactivity
Human
Validated Application
Western Blot
Immunoprecipitation
Host
Mouse
Clonality
Monoclonal
Clone ID
73P21
Target Gene
PRAS40
Gene ID
84335
Label or Dye
Unconjugated
Research Category
Apoptosis
Signal Transduction
Other Additives
Sodium Azide Preservative
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

AHO1031
6d65593971573152f4f466b4e1e5c782_CmgtBuyProduct

LanthaScreen® Akt HEK 293E Cell Line

LanthaScreen® GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. The PI3K⁄AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. Because of t ...

Specifications

System
LanthaScreen®
Readout
TR-FRET
Reporter
GFP (EmGFP)
Cell Line
293E (HEK)
Cell State
Dividing Cells
Antagonists
LY294002
PI-103
Gene Symbol
AKT1
Primary Agonist
IGF-1
Detection Method
Fluorescent
Druggable Target
Signaling Pathway
Kinases
Optimal Response
IGF-1 (EC50 = 0.42 µg/ml)
Alternate Agonists
Insulin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1615
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AKT (total) Singleplex Bead Kit (Novex®)

This Singleplex bead kit comprises the analyte specific components for the measurement of total Akt in cell lysates or tissue homogenates. This singleplex bead kit may be multiplexed with other phospho-specific and total protein singleplex bead kits available from Invitrogen, bu ...

Specifications

Marker
Akt (total)
System
Luminex®
Gene ID
207
Species
Rat
Mouse
Human
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
14
Gene Symbol
AKT1
Sensitivity
0.2 ng/ml
Target Gene
v-akt murine thymoma viral oncogene homolog 1
Gene Aliases
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NM_001014431.1
NP_001014432.1
NM_005163.2
NP_005154.2
NM_001014432.1
NP_001014431.1
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0091
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AKT Pathway Phospho 7-Plex Panel (Novex®)

The AKT Pathway Phospho 7-Plex Panel for the Luminex® platform is specifically designed for quantifying the phosphorylated proteins IR[pYpY1162/1163], IGF-1R[pYpY1135/1136], IRS-1[pS312], Akt[pS473], PRAS40[pT246], p70S6K[pTpS421/424], and GSK-3β[pS9] in cell lysates and tissue h ...

Specifications

Marker
Akt [pS473]
PRAS40 [pT246]
p70S6K [pTpS421⁄ 424]
IGF-1R [pYpY1135⁄1136]
GSK-3β [pS9]
IRS-1 [pS312]
IR [pYpY1162⁄1163]
System
Luminex®
Gene ID
207, 2932, 3480, 3643, 3667, 6198, 84335, 11651, 56637, 16001, 16337, 16367, 72508
67605 24185, 84027, 25718, 24954, 25467, 83840, 292887
Species
Rat
Mouse
Human
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
See Product Insert
Gene Symbol
Irs1
AKT1
AKT1S1
GSK3B
IRS1
IGF1R
INSR
RPS6KB1
Akt1
Igf1r
Gsk3b
Rps6kb1
Insr
Akt1s1
Sensitivity
See Product Insert
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0001
0829ddbe39a9d92230e2b36eca12c3f7_CmgtBuyProduct

AKT Pathway Total 7-Plex Panel (Novex®)

The AKT Pathway Total 7-Plex Panel for the Luminex® platform is specifically designed for quantifying the Total IR, IGF-1R, IRS-1, Akt, PRAS40, p70S6K, and GSK-3β in cell lysates and tissue homogenates. The antibodies used in this assay are human, mouse and rat cross-reactive. Th ...

Specifications

Marker
GSK-3β
IGF-1R
Akt
p70S6K
IRS-1
IR
PRAS40
System
Luminex®
Gene ID
16367, 72508, 67605, 24185, 84027, 25718, 24954, 25467, 83840, 292887
207, 2932, 3480, 3643, 3667, 6198, 84335, 11651, 56637, 16001, 16337
Species
Rat
Mouse
Human
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
See Product Insert
Gene Symbol
Irs1
AKT1
AKT1S1
GSK3B
IRS1
IGF1R
INSR
RPS6KB1
Akt1
Igf1r
Gsk3b
Rps6kb1
Insr
Akt1s1
Sensitivity
See Product Insert
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0002
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PRAS40 (total) Singleplex Bead Kit (Novex®)

This Singleplex bead kit comprises the analyte specific components for the measurement of total PRAS40 (Proline-Rich Akt Substrate 40) in cell lysates and tissue homogenates. This singleplex bead kit may be multiplexed with other phospho-specific and total protein singleplex bea ...

Specifications

Marker
PRAS40 (total)
System
Luminex®
Gene ID
84335
Species
Rat
Mouse
Human
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
74
Gene Symbol
AKT1S1
Sensitivity
0.03 ng/ml
Target Gene
AKT1 substrate 1 (proline-rich)
Gene Aliases
Lobe, MGC2865, PRAS40
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Adaptor Proteins
Accession Number
NP_001092102.1
NP_115751.2
NP_001092103.1
NM_032375.3
NM_001098632.1
NM_001098633.1
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0411
5bd9e76db0edb2df1eb3dc6e9637c93f_CmgtBuyProduct

AKT (total) Human ELISA Kit (Novex®)

The AKT (Total) ELISA research use only kit is designed to detect and quantify the level of AKT protein regardless of phosphorylation state. This assay is intended for the detection of AKT (Total) from human, mouse, or rat cell lysates using 96-well plates and a microplate reader ...

Specifications

Marker
Akt
Gene ID
10000, 207, 208
Species
Mouse
Rat
Human
Platform
Microplate Reader
Gene Symbol
AKT2
AKT3
AKT1
Sensitivity
<0.1 ng/mL (1500 cells)
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
0.3-20 ng⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Serine⁄Threonine Kinases
Cross-Reactivity
Rat
Mouse
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

KHO0101
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