PRAS40 [pT246] Polyclonal Antibody, Rabbit

This purified, unconjugated Rabbit anti-PRAS40 [pT246] polyclonal antibody is specific to human and mouse PRAS40 [pT246]. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. Rabbit anti-PRAS40 [pT246] polyclonal antibody is us ...

Specifications

Reactivity
Human
Mouse
Validated Application
Immunohistochemistry
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
PRAS40 [pT246]
Gene ID
84335, 67605
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Purified
Research Category
Signal Transduction
Apoptosis
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

441100G
f2e652b3f9c4f7b4f3ea7eeb975dfbc3_CmgtBuyProduct

PRAS40 Mouse Monoclonal Antibody (clone 73P21)

This mouse anti-PRAS40 monoclonal antibody is specific to human PRAS40. PRAS40 monoclonal antibody recognizes the expressed product of the AKT1S1 gene. PRAS40 is a 40kDa substrate of AKT. Activated AKT phosphorylates PRAS40 on threonine 246, enabling PRAS40 to bind to 14-3-3. Val ...

Specifications

Reactivity
Human
Validated Application
Western Blot
Immunoprecipitation
Host
Mouse
Clonality
Monoclonal
Clone ID
73P21
Target Gene
PRAS40
Gene ID
84335
Label or Dye
Unconjugated
Research Category
Apoptosis
Signal Transduction
Other Additives
Sodium Azide Preservative
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

AHO1031
6d65593971573152f4f466b4e1e5c782_CmgtBuyProduct

AKT Pathway Phospho 7-Plex Panel

The AKT Pathway Phospho 7-Plex Panel for the Luminex® platform is specifically designed for quantifying the phosphorylated proteins IR[pYpY1162/1163], IGF-1R[pYpY1135/1136], IRS-1[pS312], Akt[pS473], PRAS40[pT246], p70S6K[pTpS421/424], and GSK-3β[pS9] in cell lysates and tissue h ...

Specifications

Marker
Akt [pS473]
PRAS40 [pT246]
p70S6K [pTpS421⁄ 424]
IGF-1R [pYpY1135⁄1136]
GSK-3β [pS9]
IRS-1 [pS312]
IR [pYpY1162⁄1163]
System
Luminex®
Gene ID
207, 2932, 3480, 3643, 3667, 6198, 84335, 11651, 56637, 16001, 16337, 16367, 72508
67605 24185, 84027, 25718, 24954, 25467, 83840, 292887
Species
Rat
Human
Mouse
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
See Product Insert
Gene Symbol
Igf1r
Akt1s1
RPS6KB1
IGF1R
GSK3B
AKT1
AKT1S1
IRS1
INSR
Akt1
Rps6kb1
Irs1
Gsk3b
Insr
Sensitivity
See Product Insert
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0001
0829ddbe39a9d92230e2b36eca12c3f7_CmgtBuyProduct

AKT Pathway Total 7-Plex Panel

The AKT Pathway Total 7-Plex Panel for the Luminex® platform is specifically designed for quantifying the Total IR, IGF-1R, IRS-1, Akt, PRAS40, p70S6K, and GSK-3β in cell lysates and tissue homogenates. The antibodies used in this assay are human, mouse and rat cross-reactive. Th ...

Specifications

Marker
GSK-3β
IGF-1R
Akt
p70S6K
IRS-1
IR
PRAS40
System
Luminex®
Gene ID
16367, 72508, 67605, 24185, 84027, 25718, 24954, 25467, 83840, 292887
207, 2932, 3480, 3643, 3667, 6198, 84335, 11651, 56637, 16001, 16337
Species
Rat
Human
Mouse
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
See Product Insert
Gene Symbol
Igf1r
Akt1s1
RPS6KB1
IGF1R
GSK3B
AKT1
AKT1S1
IRS1
INSR
Akt1
Rps6kb1
Irs1
Gsk3b
Insr
Sensitivity
See Product Insert
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0002
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LanthaScreen® PRAS40 293E Cell Line

The PI3K/AKT pathway is activated by various stimuli (including insulin and IGF-1) and mediates signals for cell growth, cell survival, translation, and inhibition of apoptosis. PRAS40 (Proline-rich AKT substrate, 40 kDa) is a cytosolic protein that is phosphorylated at position ...

Specifications

System
LanthaScreen®
Readout
End Point
Reporter
GFP (EmGFP)
Cell State
Dividing Cells
Antagonists
IGF-1
Gene Symbol
PRAS40
Primary Agonist
Insulin
Druggable Target
Signaling Pathway
Kinases
Alternate Agonists
Insulin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1528
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PRAS40 (total) Singleplex Bead Kit

This Singleplex bead kit comprises the analyte specific components for the measurement of total PRAS40 (Proline-Rich Akt Substrate 40) in cell lysates and tissue homogenates. This singleplex bead kit may be multiplexed with other phospho-specific and total protein singleplex bea ...

Specifications

Marker
PRAS40 (total)
System
Luminex®
Gene ID
84335
Species
Rat
Human
Mouse
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
74
Gene Symbol
AKT1S1
Sensitivity
0.03 ng/ml
Target Gene
AKT1 substrate 1 (proline-rich)
Gene Aliases
Lobe, MGC2865, PRAS40
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Adaptor Proteins
Accession Number
NP_001092102.1
NP_001092103.1
NM_001098633.1
NP_115751.2
NM_001098632.1
NM_032375.3
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0411
5bd9e76db0edb2df1eb3dc6e9637c93f_CmgtBuyProduct

AKT Pathway Phospho Magnetic 7-Plex Panel

The AKT Pathway Phospho 7-Plex Magnetic Panel for the Luminex® platform is specifically designed for quantifying the phosphorylated proteins IR[pYpY1162/1163], IGF-1R[pYpY1135/1136], IRS-1[pS312], Akt[pS473], PRAS40[pT246], p70S6K[pTpS421/424], and GSK-3β[pS9] in cell lysates and ...

Specifications

Marker
Akt [pS473]
PRAS40 [pT246]
IGF-1R [pYpY1135⁄1136]
GSK-3β [pS9]
IRS-1 [pS312]
IR [pYpY1162⁄1163]
p70S6K [pTpS421⁄ 424]
Gene ID
207, 2932, 3480, 3643, 3667, 6198, 84335, 11651, 56637, 16001, 16337, 16367, 72508, 67605
24185, 84027, 25718, 24954, 25467, 83840, 292887
Species
Rat
Human
Mouse
Platform
MAGPIX® System
FlexMAP® 3D™ System
Luminex® 200™ System
Luminex® Instruments
Bead Type
Magnetic
Gene Symbol
Igf1r
Rps6kb1
Akt1
INSR
IRS1
AKT1
AKT1S1
GSK3B
IGF1R
RPS6KB1
Akt1s1
Gsk3b
Irs1
Insr
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Cell Lysates
Cell Extracts
Tissue Homogenates
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0001M
1d87bd1697571ec61fa24a87797e8098_CmgtBuyProduct

PRAS40 [pT246] Monoclonal Antibody (clone 8H13L13), ABfinity™ Recombinant

This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes Proline Rich Akt Substrate of 40 kDa (PRAS40 [pT246]) in human samples. ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Immunofluorescence
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
8H13L13
Antibody Fragment
Whole Antibody
Target Gene
Proline-rich AKT substrate
Gene ID
84335
Gene Symbol
AKT1S1
Accession Number
Q96B36
Label or Dye
Unconjugated
Purification
Purified
Concentration
0.5 mg⁄ml
Form
Liquid
Neutralizing Antibody
No
Research Category
Signal Transduction
Apoptosis
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

701058
d5ffa623b56e225005868c183357e133_CmgtBuyProduct

PRAS40 [pT246] Rabbit Polyclonal Antibody, Alexa Fluor® 488 Conjugate

PRAS40 [pT246) ALEXA CONJ; Alexa Fluor 488 conjugated Polyclonal antibody specific to Human, (Mouse) PRAS40 [pT246]. This antibody is validated for use in Immunocytochemistry/ Immunofluorescence. Anti-PRAS40 [pT246] recognizes the expressed product of the AKT1S1 gene. ...

Specifications

Reactivity
Human
Mouse
Validated Application
Immunohistochemistry
Host
Rabbit
Clonality
Polyclonal
Target Gene
PRAS40 [pT246]
Gene ID
84335
Label or Dye
Alexa Fluor® 488
Excitation⁄Emission (nm)
488⁄519
Target Modification State
Phospho
Purification
Antiserum
Research Category
Signal Transduction
Apoptosis
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

441100A1
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LanthaScreen® Tb-anti-PRAS40 [pSer183] Antibody Kit

The LanthaScreen® Tb-anti-PRAS40[pSer183] antibody has been designed and validated for use in the LanthaScreen® PRAS40 HEK 293E Cellular Assay, to facilitate in-house assay configuration and screening. These antibodies may be accessed in small scale and screening quantities. ...

Specifications

Host
Mouse
Gene ID
84335
Platform
Microplate Reader
Clonality
Monoclonal
Technique
TR-FRET
Reactivity
Human
Gene Symbol
AKT1S1
Label or Dye
Tb (Terbium)
Concentration
0.5 mg⁄ml
Target Molecule
PRAS40 [pSer183]
Antibody Fragment
Whole Antibody
SubClass (Isotype)
IgG
High Throughput Compatibility
High Throughput-Compatible
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV5580
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AKT Pathway Total 7-Plex Magnetic Panel

The AKT Pathway Total 7-Plex Magnetic Panel for the Luminex® platform is specifically designed for quantifying the Total IR, IGF-1R, IRS-1, Akt, PRAS40, p70S6K, and GSK-3β in cell lysates and tissue homogenates. The antibodies used in this assay are human, mouse and rat cross-rea ...

Specifications

Marker
GSK-3β
IGF-1R
p70S6K
IRS-1
IR
PRAS40
Akt
System
Luminex®
Gene ID
207, 2932, 3480, 3643, 3667, 6198, 84335, 11651, 56637, 16001, 16337, 16367, 72508, 67605
24185, 84027, 25718, 24954, 25467, 83840, 292887
Species
Rat
Human
Mouse
Platform
Luminex® Instruments
Bead Type
Magnetic
Gene Symbol
Igf1r
Rps6kb1
Akt1
INSR
IRS1
AKT1
AKT1S1
GSK3B
IGF1R
RPS6KB1
Akt1s1
Gsk3b
Irs1
Insr
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Research Category
Signal Transduction
Sample Type (Specific)
Tissue (Mammalian)
Mammalian Cells
Cell Lysates
Cell Extracts
Tissue Homogenates
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0002M
6df82f5ba0cf51e1f4506f5140a71f55_CmgtBuyProduct

PRAS40 (total) Human ELISA Kit

The PRAS40 (Total) Human ELISA kit is designed to detect and quantify the level of PRAS40 protein. This assay is intended for the detection of PRAS40 from human cell lysates using 96-well plates and a microplate reader. ...

Specifications

Marker
PRAS40
Gene ID
84335
Species
Human
Platform
Microplate Reader
Gene Symbol
AKT1S1
Sensitivity
<10 pg/mL
Target Gene
AKT1 substrate 1 (proline-rich)
Gene Aliases
Lobe, MGC2865, PRAS40
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
31-2000 pg⁄ml
Sample Volume
100 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Adaptor Proteins
Accession Number
NP_001092102.1
NP_115751.2
NP_001092103.1
NM_032375.3
NM_001098632.1
NM_001098633.1
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

KHO0411
37d12a23db59230a1f7c98f243f9ee0f_CmgtBuyProduct

PRAS40 [pT246] Singleplex Bead Kit

This Singleplex bead kit comprises the analyte specific components for the measurement of PRAS40 (Proline-Rich Akt Substrate 40) when phosphorylated at threonine 246 in cell lysates and tissue homogenates. This singleplex bead kit may be multiplexed with other phospho-specific a ...

Specifications

Marker
PRAS40 [pT246]
System
Luminex®
Gene ID
84335, 67605, 292887
Species
Rat
Human
Mouse
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
74
Gene Symbol
Akt1s1
AKT1S1
Sensitivity
4.4 U/ml
Target Gene
AKT1 substrate 1 (proline-rich)
Gene Aliases
1110012J22Rik, AI227026, Lobe, Lobel, PRAS40, PRAS40, Lobe, MGC2865, PRAS40
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Adaptor Proteins
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0421
acb4bda0fe050e9a3c1aa167a9d88620_CmgtBuyProduct

PRAS40 (total) Mouse ELISA Kit

The PRAS40 (Total) Mouse ELISA kit is designed to detect and quantify the level of PRAS40 protein. This assay is intended for the detection of PRAS40 from mouse, human and rat cell lysates using 96-well plates and a microplate reader. ...

Specifications

Marker
PRAS40
Gene ID
84335, 292887
Species
Rat
Human
Mouse
Platform
Microplate Reader
Gene Symbol
Akt1s1
AKT1S1
Sensitivity
<200 pg⁄mL
Target Gene
AKT1 substrate 1 (proline-rich)
Gene Aliases
PRAS40, Lobe, MGC2865, PRAS40
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
0.313-20 ng⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Adaptor Proteins
Cross-Reactivity
Human
Rat
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

KMO0411
4820e894a22e8cb8593bf4ae54ea6a82_CmgtBuyProduct

PRAS40 [pT246] Human ELISA Kit

The PRAS40 [pT246] Human ELISA research use only kit is designed to detect and quantify the level of PRAS40 protein phosphorylated at threonine residue 246. This assay is intended for the detection of PRAS40 [pT246] from human cell lysates using 96-well plates and a microplate re ...

Specifications

Marker
PRAS40
Gene ID
84335
Species
Human
Platform
Microplate Reader
Gene Symbol
AKT1S1
Sensitivity
0.5 U⁄mL (1000 cells)
Target Gene
AKT1 substrate 1 (proline-rich)
Gene Aliases
Lobe, MGC2865, PRAS40
Label or Dye
HRP (Horseradish Peroxidase)
Concentration
1.6 U⁄ml
Sample Volume
10 µl
Protein Family
Kinases & Inhibitors
Incubation Time
4 hrs
Protein Subtype
Adaptor Proteins
Accession Number
NP_001092102.1
NM_001098633.1
NP_115751.2
NM_032375.3
NP_001092103.1
NM_001098632.1
Detection Method
Colorimetric
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

KHO0421
e06ded4bde3a25bb13e02e634168d19a_CmgtBuyProduct