Ion AmpliSeq™ RNA Cancer Panel

The Ion AmpliSeq™ RNA Cancer Panel is a screening tool designed against 50 oncogenes/tumor suppressor genes found in the COSMIC database. This panel has been developed as an RNA complement to the DNA-focused Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of ...

Specifications

Platform
Ion PGM™ System
Library Type
Targeted Sequencing Library
Workflow Step
Target Selection
Starting Material
Total RNA
Sequencing Type
Transcriptome Sequencing
Species
Human
Technique
Amplicon Sequencing
Sample Type (Specific)
RNA (>500 bases)
Tissue (FFPE)
Starting Material (Amount)
500 pg of total RNA from fresh frozen tissue or 5 ng of RNA from FFPE samples
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Ion AmpliSeq™ RNA Library Kit

The Ion AmpliSeq™ RNA Library Kit is a library construction kit designed for rapid preparation of RNA amplicon libraries compatible with Ion AmpliSeq™ RNA ready-to-use or custom panels for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ system. A list of TaqM ...

Specifications

Platform
Ion PGM™ System
Library Type
Targeted Sequencing Library
Workflow Step
Library Generation
Starting Material
Total RNA
Sequencing Type
Transcriptome Sequencing
Species
Human
Technique
Amplicon Sequencing
Multiplex Capability
Compatible with Ion Xpress™ Barcode Kits
Sample Type (Specific)
Tissue (FFPE)
Total RNA
Starting Material (Amount)
500 pg of total RNA from fresh frozen tissue or 5 ng of RNA from FFPE samples
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4482752
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Ion AmpliSeq™ Exome RDY - OT2 Kit 4x2

The Ion AmpliSeq™ Exome RDY - OT2 Kit enables the fastest and simplest method of exome enrichment, now even easier to use with oligo pools for ultra-high multiplex PCR exome enrichment dried down in 96-well plates. With half the number of target amplification pipetting steps comp ...

Specifications

New Product
New additions to lifetechnologies.com
Platform
Ion OneTouch™ 2 System
Ion Proton™ System
Library Type
Targeted Sequencing Library
Workflow Step
Library Generation
Target Selection
Sample Enrichment
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Final Product
PCR Amplicons (Normalized)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4489061
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Ion AmpliSeq™ Exome RDY - OT2 Kit 1x8

The Ion AmpliSeq™ Exome RDY - OT2 Kit enables the fastest and simplest method of exome enrichment, now even easier to use with oligo pools for ultra-high multiplex PCR exome enrichment dried down in a 96-well plate. With half the number of target amplification pipetting steps com ...

Specifications

New Product
New additions to lifetechnologies.com
Platform
Ion OneTouch™ 2 System
Ion Proton™ System
Library Type
Targeted Sequencing Library
Workflow Step
Library Generation
Target Selection
Sample Enrichment
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Final Product
PCR Amplicons (Normalized)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4489837
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Ion AmpliSeq™ Exome RDY - IC Kit 1x8

The Ion AmpliSeq™ Exome RDY - IC Kit enables the fastest and simplest method of exome enrichment, now even easier with oligo pools for ultra-high multiplex PCR exome enrichment dried down in a 96-well plate. With half the number of target amplification pipetting steps compared to ...

Specifications

New Product
New additions to lifetechnologies.com
Platform
Ion OneTouch™ 2 System
Ion Proton™ System
Library Type
Targeted Sequencing Library
Workflow Step
Library Generation
Target Selection
Sample Enrichment
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Final Product
PCR Amplicons (Normalized)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A25908
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Ion AmpliSeq™ Exome RDY - IC Kit 4x2

The Ion AmpliSeq™ Exome RDY - IC Kit enables the fastest and simplest method of exome enrichment, now even easier with oligo pools for ultra-high multiplex PCR exome enrichment dried down in 96-well plates. With half the number of target amplification pipetting steps compared to ...

Specifications

New Product
New additions to lifetechnologies.com
Platform
Ion OneTouch™ 2 System
Ion Proton™ System
Library Type
Targeted Sequencing Library
Workflow Step
Library Generation
Target Selection
Sample Enrichment
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Final Product
PCR Amplicons (Normalized)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A25909
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Ion AmpliSeq™ RNA Apoptosis Panel

The Ion AmpliSeq™ RNA Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor-mediated apoptosis, c-Myc, and p53-mediated apoptosis. ...

Specifications

Platform
Ion PGM™ System
Library Type
Targeted Sequencing Library
Workflow Step
Target Selection
Starting Material
Total RNA
Sequencing Type
Transcriptome Sequencing
Species
Human
Technique
Amplicon Sequencing
Sample Type (Specific)
RNA (>500 bases)
Tissue (FFPE)
Starting Material (Amount)
500 pg of total RNA from fresh frozen tissue or 5 ng of RNA from FFPE samples
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4482571
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Ion AmpliSeq™ Exome Kit 4xDuo

The Ion AmpliSeq™ Exome Kit 4xDuo enables the fastest and simplest method of exome enrichment to accelerate your human disease research, and is intended for use with the Ion PI™ Template OT2 200 Kit v3 and Ion PI™ Sequencing 200 Kit v3. Producing exome libraries in under 6 hours ...

Specifications

Platform
Ion Proton™ System
Library Type
Targeted Sequencing Library
Workflow Step
Target Selection
Sample Enrichment
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Final Product
PCR Amplicons (Normalized)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Ion AmpliSeq™ Exome Kit 8xTrio

The Ion AmpliSeq™ Exome Kit 8xTrio enables the fastest and simplest method of exome enrichment to accelerate your human disease research, and is intended for use with the Ion PI™ Template OT2 200 Kit v3 and Ion PI™ Sequencing 200 Kit v3. Producing exome libraries in under 6 hour ...

Specifications

Platform
Ion Proton™ System
Library Type
Targeted Sequencing Library
Workflow Step
Target Selection
Sample Enrichment
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Final Product
PCR Amplicons (Normalized)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Ion AmpliSeq™ Dynabeads® Cleanup Module

The Ion AmpliSeq™ Dynabeads® Cleanup Module is a magnetic bead-based purification kit used in the construction of RNA libraries for sequencing on the Ion Personal Genome Machine® (PGM™) or Ion Proton™ sequencer. This module is included in the Ion AmpliSeq™ RNA Library Kit, but m ...

Specifications

Platform
Ion PGM™ System
Final Product
cDNA Library
Isolation Technology
Magnetic Bead
Sample Type (General)
RNA
Sample Type (Specific)
RNA (>500 bases)
Tissue (FFPE)
Fine Needle Aspirates (FNA)
High Throughput Compatibility
High Throughput-Compatible
Not High Throughput-Compatible (Manual)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4482437
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Ion AmpliSeq™ Library Kit 2.0

The Ion AmpliSeq™Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ready-to-use panels and custom primer pools, for sequencing on the Ion Personal Genome Machine™ (PGM™) System. ...

Specifications

Platform
Ion PGM™ System
Library Type
Targeted Sequencing Library
Workflow Step
Library Generation
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Technique
Post-Light™ Ion Semiconductor Sequencing
Amplicon Sequencing
Multiplex Capability
12-plex to 3072-plex per well
1-96 barcodes
Sample Type (Specific)
Tissue (FFPE)
DNA (Genomic)
Recommended Applications
Targeted Resequencing
Starting Material (Amount)
10 ng per primer pool
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Ion AmpliSeq™ Cancer Hotspot Panel v2

The Ion AmpliSeq™Cancer Hotspot Panel v2 is a single pool of primers used to perform multiplex PCR for preparation of amplicon libraries from genomic "hot spot" regions that are frequently mutated in human cancer genes (download the list of targeted genes and mutations for the Io ...

Specifications

Platform
Ion PGM™ System
Library Type
Targeted Sequencing Library
Workflow Step
Target Selection
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Species
Human
Technique
Post-Light™ Ion Semiconductor Sequencing
Amplicon Sequencing
Multiplex Capability
207 amplicons
Sample Type (Specific)
Whole Blood
Double-Stranded DNA (dsDNA)
Clinical Sample
Cell Cultures
DNA (Genomic)
Blood
Fine Needle Aspirates (FNA)
Tissue (FFPE)
Starting Material (Amount)
10 ng DNA
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4475346
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Ion AmpliSeq™ Comprehensive Cancer Panel

The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all- ...

Specifications

Platform
Ion PGM™ System
Library Type
Targeted Sequencing Library
Workflow Step
Target Selection
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Species
Human
Technique
Post-Light™ Ion Semiconductor Sequencing
Amplicon Sequencing
Multiplex Capability
Multiplex PCR: 16,000 primer pairs in 4 pools
Sample Type (Specific)
Whole Blood
Double-Stranded DNA (dsDNA)
Clinical Sample
Blood
Cell Cultures
DNA (Genomic)
Fine Needle Aspirates (FNA)
Tissue (FFPE)
Starting Material (Amount)
40 ng
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Ion AmpliSeq™ Inherited Disease Panel

The Ion AmpliSeq™ Inherited Disease Panel provides highly multiplexed target selection of all exons of over genes that are mutated in over 700 unique inherited diseases, according to NCBI ClinVar database (May 2012). Consulting with clinical molecular geneticists involved with in ...

Specifications

Platform
Ion PGM™ System
Library Type
Targeted Sequencing Library
Workflow Step
Target Selection
Starting Material
DNA
Sequencing Type
Genome & DNA Sequencing
Species
Human
Technique
Post-Light™ Ion Semiconductor Sequencing
Amplicon Sequencing
Multiplex Capability
Multiplex PCR: 10,500 primer pairs in 3 pools
Sample Type (Specific)
Buccal Swab
Double-Stranded DNA (dsDNA)
Clinical Sample
Blood
Cell Cultures
DNA (Genomic)
Whole Blood
Buffy Coat
Starting Material (Amount)
30 ng
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4477686
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