Genomics and proteomics search results

LanthaScreen® ERK2 U2OS Cell Line

The Map Kinase (MapK) signal transduction cascade is activated by growth factors such as EGF, PDGF, and HGF. Binding of these factors to their respective cell surface receptors results in the initiation of receptor tyrosine kinase activity, which leads to the sequential phospho-a ...

Specifications

System
LanthaScreen®
Reporter
GFP (EmGFP)
Cell Line
U2OS
Technique
TR-FRET
Cell State
Dividing Cells
Antagonists
U0126
Gene Symbol
ERK2
Primary Agonist
EGF
Detection Method
Fluorescent
Druggable Target
Kinases
Signaling Pathway
Optimal Response
EGF (EC50 = 0.83 ng/ml)
Alternate Agonists
PDGF
Orthovanadate
HGF
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1587
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MAPK1 (ERK2) Recombinant Human Protein, Inactive

MAPK1 (ERK2) is a serine⁄threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ...

Specifications

Gene ID
5594
Species
Human
Target Gene
mitogen-activated protein kinase 1
Gene Aliases
MAPK1 (ERK2)
Kinase Group
Ser⁄Thr Kinases: CMGC Group
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NP_620407
Molecular Weight
69.7 kDa
Research Category
Signal Transduction
Validated Application
Kinase Assay
Protein Tag or Fusion
GST Tag
Expression
E. coli
Kinase Family
MAP Kinase Family
Protein Form
Full Length
Inactive
Recombinant
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV3314
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BacMam ERK2 [pThr185/pTyr187] Cellular Assay Kit (Invitrogen™)

The BacMam ERK2 [pThr185⁄pTyr187] Cellular Assay Kit is a high-throughput screening (HTS) compatible cellular immunoassay for the measuring of phosphorylation of GFP-ERK2. The BacMam ERK2 Cellular Assay Kit can be used with numerous transformed cell lines, primary cells, and ste ...

Specifications

System
LanthaScreen®
Readout
End Point
Technique
TR-FRET
Cell State
Cells Not Included
Gene Symbol
ERK2
Delivery Method
Baculovirus (BacMam System)
Detection Method
Fluorescent
Druggable Target
Kinases
GPCRs
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A12888
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LanthaScreen® Tb-anti-ERK2 [pThr185⁄pTyr187] Antibody Kit

To facilitate in-house kinase assay configuration and screening, we have developed a selection of Tb-labeled phospho-specific antibodies, which may be accessed in small scale and screening quantities. ...

Specifications

Form
Liquid
Host
Rabbit
Platform
Microplate Reader
Clonality
Monoclonal
Reactivity
Human
Label or Dye
Tb (Terbium)
Purification
Purified
Concentration
0.5 mg⁄ml
Antibody Fragment
Whole Antibody
SubClass (Isotype)
IgG
High Throughput Compatibility
High Throughput-Compatible
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV5270
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LanthaScreen® ERK2 A375 Cell Line

The Map Kinase (MapK) signal transduction cascade is activated by growth factors such as EGF, PDGF, and HGF. Binding of these factors to their respective cell surface receptors results in the initiation of receptor tyrosine kinase activity, which leads to the sequential phospho- ...

Specifications

System
LanthaScreen®
Readout
End Point
Cell Line
A375
Technique
TR-FRET
Cell State
Dividing Cells
Antagonists
Raf Inhibitor I
Gene Symbol
ERK2
Primary Agonist
Constitutive
Detection Method
Fluorescent
Druggable Target
Kinases
Signaling Pathway
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1798
779ab5a0fd842eb63dcf966673549a89_CmgtBuyProduct

MAPK1 (ERK2) Recombinant Human Protein (Life Technologies™)

MAPK1 (ERK2) is a serine⁄threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ...

Specifications

Form
Frozen
Species
Human
Gene Aliases
MAPK1 (ERK2)
Kinase Group
Ser⁄Thr Kinases: CMGC Group
Accession Number
NP_620407
Molecular Weight
69.7 kDa
Research Category
Drug Development
Validated Application
Kinase Assay
Protein Tag or Fusion
GST Tag
Expression
E. coli
Kinase Family
MAP Kinase Family
Protein Form
Full Length
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV3595
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LanthaScreen® Tb-anti-ERK2 [pThr185⁄pTyr187] Antibody

A part of the MAPK Pathway, ERK2 is phosphorylated at Thr185 and Tyr187 by the mitogen-activated protein kinase kinases 2 (MAP2K2) .
For use in combination with the LanthaScreen® ERK2 A375 cell line (K1798) to evaluate inhibitors in medium to high throughput screening applicati ...

Specifications

Readout
End Point
Reactivity
Human
Target Gene
Ser/Thr Kinases, Tyrosine Kinases
Label or Dye
Tb (Terbium)
Excitation⁄Emission (nm)
340⁄490, 520
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV5210
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p42 MAP Kinase / ERK2 / MAPK1 Antibody (6F8) (Thermo Scientific™)

MA1-099 has been successfully used in Western blot, ChIP, immunofluorescence, immunohistochemistry and immunoprecipitation applications, and reacts with human, dog, rat, mouse and monkey samples. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
MA1099
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p44/42 MAPK (ERK1/2) (pT202/pY204), Oligoclonal Antibody, ABfinity™ Recombinant (Novex®)

This purified oligoclonal recombinant rabbit IgG antibody recognizes human p44/42 MAPK (ERK1/2) [pT202/pY204]. ...

Specifications

Reactivity
Human
Validated Application
Western Blot
ELISA
Immunocytochemistry
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Clone ID
6HCLC
Antibody Fragment
Whole Antibody
Target Gene
MAPK (ERK1/2) p44/42 (pT202/pY204)
Gene ID
5595
Gene Symbol
MAPK3
Accession Number
P27361
Gene Aliases
ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
Label or Dye
Unconjugated
Target Modification State
Phospho
Form
Liquid
Buffer
Phosphate buffered saline (PBS) with 0.09% sodium azide
Other Additives
Sodium Azide Preservative
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

710318
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ERK1/2 [pTpY185/187] Polyclonal Antibody, Rabbit (Novex®)

This purified Rabbit Anti-ERK 1/2 [pTpY185/187] Polyclonal Antibody is specific to human, mouse, and rat ERK 1/2 [pT185/pY187]. Anti-ERK1/2 [pT185/pY187] antibody recognizes the product of the MAPK3 gene. ERK (extracellular signal-regulated kinase), also known as MAPK (mitogen-ac ...

Specifications

Reactivity
Human
Mouse
Rat
Xenopus
Drosophila
C. elegans
Chicken
Bovine
Validated Application
Western Blot
Immunohistochemistry
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ERK1/2 [pTpY185/187]
Gene ID
5595
Label or Dye
Unconjugated
Target Modification State
Phospho
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44680G
79a10412807dc5fd8bdc1cdfa49f0591_CmgtBuyProduct

CellSensor® AP-1-bla ME-180 Cell Line

The CellSensor® AP-1-bla ME-180 Cell Line contains a beta-lactamase reporter gene under control of the AP-1 response element stably integrated into ME-180 cells. The cell line was created through FACS sorting of cells responsive to stimulation of the AP-1 pathway with Epidermal G ...

Specifications

System
CellSensor™
Cell State
Dividing Cells
Druggable Target
Signaling Pathway
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1661
503bdf3b8dff78dd86629161dcd64e32_CmgtBuyProduct

ERK1/2 [pTpY185/187] Singleplex Bead Kit (Novex®)

This Singleplex bead kit comprises the analyte specific components for the measurement of ERK1⁄2 (Extracellular signal-Regulated protein Kinase) when phosphorylated at threonine 185 and tyrosine 187 in cell lysates or tissue homogenates. This singleplex bead kit may be multiplex ...

Specifications

Marker
ERK1⁄2 [pTpY185⁄187]
System
Luminex®
Gene ID
5595
Species
Human
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
57
Gene Symbol
MAPK3
Sensitivity
0.5 U/ml
Target Gene
mitogen-activated protein kinase 3
Gene Aliases
ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, MGC20180, PRKM3, p44-ERK1, p44-MAPK
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NM_002746.2
NM_001040056.1
NP_001103361.1
NP_001035145.1
NP_002737.2
NM_001109891.1
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Cell Extracts
Tissue Homogenates
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0241
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ERK1/2 (pan) Polyclonal Antibody, Rabbit (Novex®)

This purified rabbit anti-ERK1/2 polyclonal antibody is specific to human, mouse, rat and bovine ERK 1+2. Anti-ERK1+2 polyclonal antibody recognizes the expressed product of the MAPK3 gene. ERK (extracellular signal regulated kinase), also known as MAPK (mitogen-activated protein ...

Specifications

Reactivity
Human
Mouse
Rat
Bovine
Validated Application
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ERK1/2 (pan)
Gene ID
5595
Label or Dye
Unconjugated
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44654G
ac53e5ebfa7e2096404b4ac75028c4ea_CmgtBuyProduct

ERK1/2 [pTpY185/187] Antibody Pair (Novex®)

This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of human phosphorylated ERK1⁄2 [pTpT185⁄187] (Extracellular signal-Regulated protein Kinase). Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use ...

Specifications

Form
Concentrated
Marker
ERK1⁄2 [pTpY185⁄187]
Gene ID
5594, 5595
Platform
Microplate Reader
Reactivity
Human
Gene Symbol
MAPK3
MAPK1
Target Gene
ERK1/2 [pTpY185/187]
Label or Dye
HRP (Horseradish Peroxidase)
Emission Class
Visible
Protein Family
Kinases & Inhibitors
Target Molecule
Proteins (Phosphoproteins)
Research Category
Signal Transduction
Validated Application
ELISA
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

CHO0095
9a4b13369cc77db592a6617750369250_CmgtBuyProduct

ERK1/2 Rabbit Polyclonal Antibody (Novex®)

Rabbit anti-MAP Kinase (ERK1+ERK2); Unconjugated Polyclonal antibody specific to Human, Mouse, Rat ERK1+2. This antibody is validated for use in Western Blot, Immunohistochemistry, Immunoassay (ELISA). Anti-ERK1+2 recognizes the expressed product of the MAPK1 gene, also known as ...

Specifications

Reactivity
Human
Mouse
Rat
Validated Application
ELISA
Immunoprecipitation
Western Blot
Immunohistochemistry
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ERK1/2
Gene ID
116590, 26413, 26417, 50689, 5594, 5595
Label or Dye
Unconjugated
Purification
Epitope Affinity Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

617400
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