Genomics and proteomics search results

LanthaScreen® ERK2 U2OS Cell Line

The Map Kinase (MapK) signal transduction cascade is activated by growth factors such as EGF, PDGF, and HGF. Binding of these factors to their respective cell surface receptors results in the initiation of receptor tyrosine kinase activity, which leads to the sequential phospho-a ...

Specifications

System
LanthaScreen®
Reporter
GFP (EmGFP)
Cell Line
U2OS
Technique
TR-FRET
Cell State
Dividing Cells
Antagonists
U0126
Gene Symbol
ERK2
Primary Agonist
EGF
Detection Method
Fluorescent
Druggable Target
Kinases
Signaling Pathway
Optimal Response
EGF (EC50 = 0.83 ng/ml)
Alternate Agonists
PDGF
Orthovanadate
HGF
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1587
01e4b1cdd67c29f4deb250e1570b7dd1_CmgtBuyProduct

LanthaScreen® ERK2 A375 Cell Line

The Map Kinase (MapK) signal transduction cascade is activated by growth factors such as EGF, PDGF, and HGF. Binding of these factors to their respective cell surface receptors results in the initiation of receptor tyrosine kinase activity, which leads to the sequential phospho- ...

Specifications

System
LanthaScreen®
Readout
End Point
Cell Line
A375
Technique
TR-FRET
Cell State
Dividing Cells
Antagonists
Raf Inhibitor I
Gene Symbol
ERK2
Primary Agonist
Constitutive
Detection Method
Fluorescent
Druggable Target
Kinases
Signaling Pathway
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1798
779ab5a0fd842eb63dcf966673549a89_CmgtBuyProduct

MAPK1 (ERK2) Recombinant Human Protein, Inactive

MAPK1 (ERK2) is a serine⁄threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ...

Specifications

Gene ID
5594
Species
Human
Target Gene
mitogen-activated protein kinase 1
Gene Aliases
MAPK1 (ERK2)
Kinase Group
Ser⁄Thr Kinases: CMGC Group
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NP_620407
Molecular Weight
69.7 kDa
Research Category
Signal Transduction
Validated Application
Kinase Assay
Protein Tag or Fusion
GST Tag
Expression
E. coli
Kinase Family
MAP Kinase Family
Protein Form
Full Length
Inactive
Recombinant
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV3314
ccba60a505e3ce5a5b692521fde8b224_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (6F8) (Thermo Scientific™)

MA1-099 has been successfully used in Western blot, ChIP, immunofluorescence, immunohistochemistry and immunoprecipitation applications, and reacts with human, dog, rat, mouse and monkey samples. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
MA1099
89e0f34dc58177adef5a5dcd18c3f2e1_CmgtBuyProduct

LanthaScreen® Tb-anti-ERK2 [pThr185⁄pTyr187] Antibody

A part of the MAPK Pathway, ERK2 is phosphorylated at Thr185 and Tyr187 by the mitogen-activated protein kinase kinases 2 (MAP2K2) .
For use in combination with the LanthaScreen® ERK2 A375 cell line (K1798) to evaluate inhibitors in medium to high throughput screening applicati ...

Specifications

Readout
End Point
Reactivity
Human
Target Gene
Ser/Thr Kinases, Tyrosine Kinases
Label or Dye
Tb (Terbium)
Excitation⁄Emission (nm)
340⁄490, 520
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV5209
837a68b76082837bf35a8711c6220479_CmgtBuyProduct

LanthaScreen® Tb-anti-ERK2 [pThr185⁄pTyr187] Antibody Kit

To facilitate in-house kinase assay configuration and screening, we have developed a selection of Tb-labeled phospho-specific antibodies, which may be accessed in small scale and screening quantities. ...

Specifications

Form
Liquid
Host
Rabbit
Platform
Microplate Reader
Clonality
Monoclonal
Reactivity
Human
Label or Dye
Tb (Terbium)
Purification
Purified
Concentration
0.5 mg⁄ml
Antibody Fragment
Whole Antibody
SubClass (Isotype)
IgG
High Throughput Compatibility
High Throughput-Compatible
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV5269
4407ad99170454f730726558c4c44835_CmgtBuyProduct

MAPK1 (ERK2) Recombinant Human Protein (Life Technologies™)

MAPK1 (ERK2) is a serine⁄threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ...

Specifications

Form
Frozen
Species
Human
Gene Aliases
MAPK1 (ERK2)
Kinase Group
Ser⁄Thr Kinases: CMGC Group
Accession Number
NP_620407
Molecular Weight
69.7 kDa
Research Category
Drug Development
Validated Application
Kinase Assay
Protein Tag or Fusion
GST Tag
Expression
E. coli
Kinase Family
MAP Kinase Family
Protein Form
Full Length
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

5e47a5d771d989d236e48f75f485ef00_CmgtBuyProduct

BacMam ERK2 [pThr185/pTyr187] Cellular Assay Kit (Invitrogen™)

The BacMam ERK2 [pThr185⁄pTyr187] Cellular Assay Kit is a high-throughput screening (HTS) compatible cellular immunoassay for the measuring of phosphorylation of GFP-ERK2. The BacMam ERK2 Cellular Assay Kit can be used with numerous transformed cell lines, primary cells, and ste ...

Specifications

System
LanthaScreen®
Readout
End Point
Technique
TR-FRET
Cell State
Cells Not Included
Gene Symbol
ERK2
Delivery Method
Baculovirus (BacMam System)
Detection Method
Fluorescent
Druggable Target
Kinases
GPCRs
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A12888
4709aec82fa07caa3ee5bf05c979192d_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (4C11) (Thermo Scientific™)

MA5-15304 targets ERK2 in IF, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples. The MA5-15304 immunogen is purified recombinant fragment of human ERK2 expressed in E. Coli. MA5-15304 detects ERK2 which has a predicted molecular ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
MA515304
3843ac9b55579283ffa623ed867e44c4_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (Thermo Scientific™)

PA5-29636 targets ERK2 in IHC (P) and WB applications and shows reactivity with Human, Rat, and mouse samples. The PA5-29636 immunogen is recombinant fragment corresponding to a region within amino acids 66 and 360 of Human ERK2. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA529636
7bc22aa5f49b556e752d51d2ff97678d_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (Thermo Scientific™)

It is not recommended to aliquot this antibody. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA517710
eedc71b84b1321c57866d3fd3737f837_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (Thermo Scientific™)

This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA518329
e41df31b70b603ae710d81b96665f10d_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (Thermo Scientific™)

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggeste ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA532396
5a3fe0f117d34e8a40f69c07fb9cc04c_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (Thermo Scientific™)

This antibody is predicted to react with bovine, mouse and rat based on sequence homology. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA514414
3253ed0e4478d3e80bee3edee63f8e1f_CmgtBuyProduct

p42 MAP Kinase / ERK2 / MAPK1 Antibody (Thermo Scientific™)

This antibody is predicted to react with bovine, chicken, canine, amphibian, porcine, rabbit and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room te ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA137640
2ed98fa47b8bc1af056b074fb8879592_CmgtBuyProduct