Genomics and proteomics search results

LanthaScreen® ERK2 U2OS Cell Line

The Map Kinase (MapK) signal transduction cascade is activated by growth factors such as EGF, PDGF, and HGF. Binding of these factors to their respective cell surface receptors results in the initiation of receptor tyrosine kinase activity, which leads to the sequential phospho-a ...

Specifications

System
LanthaScreen®
Reporter
GFP (EmGFP)
Cell Line
U2OS
Technique
TR-FRET
Cell State
Dividing Cells
Antagonists
U0126
Gene Symbol
ERK2
Primary Agonist
EGF
Detection Method
Fluorescent
Druggable Target
Signaling Pathway
Kinases
Optimal Response
EGF (EC50 = 0.83 ng/ml)
Alternate Agonists
Orthovanadate
PDGF
HGF
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1587
01e4b1cdd67c29f4deb250e1570b7dd1_CmgtBuyProduct

LanthaScreen® ERK2 A375 Cell Line

The Map Kinase (MapK) signal transduction cascade is activated by growth factors such as EGF, PDGF, and HGF. Binding of these factors to their respective cell surface receptors results in the initiation of receptor tyrosine kinase activity, which leads to the sequential phospho- ...

Specifications

System
LanthaScreen®
Readout
End Point
Cell Line
A375
Technique
TR-FRET
Cell State
Dividing Cells
Antagonists
Raf Inhibitor I
Gene Symbol
ERK2
Primary Agonist
Constitutive
Detection Method
Fluorescent
Druggable Target
Signaling Pathway
Kinases
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1798
779ab5a0fd842eb63dcf966673549a89_CmgtBuyProduct

MAPK1 (ERK2) Recombinant Human Protein (Life Technologies™)

MAPK1 (ERK2) is a serine⁄threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ...

Specifications

Form
Frozen
Species
Human
Gene Aliases
MAPK1 (ERK2)
Kinase Group
Ser⁄Thr Kinases: CMGC Group
Accession Number
NP_620407
Molecular Weight
69.7 kDa
Research Category
Drug Development
Validated Application
Kinase Assay
Protein Tag or Fusion
GST Tag
Expression
E. coli
Kinase Family
MAP Kinase Family
Protein Form
Full Length
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV3595
ec138a7619ea9798fc0cf90f019395d0_CmgtBuyProduct

BacMam ERK2 [pThr185/pTyr187] Cellular Assay Kit (Invitrogen™)

The BacMam ERK2 [pThr185⁄pTyr187] Cellular Assay Kit is a high-throughput screening (HTS) compatible cellular immunoassay for the measuring of phosphorylation of GFP-ERK2. The BacMam ERK2 Cellular Assay Kit can be used with numerous transformed cell lines, primary cells, and ste ...

Specifications

System
LanthaScreen®
Readout
End Point
Technique
TR-FRET
Cell State
Cells Not Included
Gene Symbol
ERK2
Delivery Method
Baculovirus (BacMam System)
Detection Method
Fluorescent
Druggable Target
GPCRs
Kinases
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A12888
4709aec82fa07caa3ee5bf05c979192d_CmgtBuyProduct

MAPK1 (ERK2) Recombinant Human Protein, Inactive

MAPK1 (ERK2) is a serine⁄threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ...

Specifications

Gene ID
5594
Species
Human
Target Gene
mitogen-activated protein kinase 1
Gene Aliases
MAPK1 (ERK2)
Kinase Group
Ser⁄Thr Kinases: CMGC Group
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NP_620407
Molecular Weight
69.7 kDa
Research Category
Signal Transduction
Validated Application
Kinase Assay
Protein Tag or Fusion
GST Tag
Expression
E. coli
Kinase Family
MAP Kinase Family
Protein Form
Full Length
Recombinant
Inactive
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV3314
ccba60a505e3ce5a5b692521fde8b224_CmgtBuyProduct

LanthaScreen® Tb-anti-ERK2 [pThr185⁄pTyr187] Antibody Kit

To facilitate in-house kinase assay configuration and screening, we have developed a selection of Tb-labeled phospho-specific antibodies, which may be accessed in small scale and screening quantities. ...

Specifications

Form
Liquid
Host
Rabbit
Platform
Microplate Reader
Clonality
Monoclonal
Reactivity
Human
Label or Dye
Tb (Terbium)
Purification
Purified
Concentration
0.5 mg⁄ml
Antibody Fragment
Whole Antibody
SubClass (Isotype)
IgG
High Throughput Compatibility
High Throughput-Compatible
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV5269
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LanthaScreen® Tb-anti-ERK2 [pThr185⁄pTyr187] Antibody

A part of the MAPK Pathway, ERK2 is phosphorylated at Thr185 and Tyr187 by the mitogen-activated protein kinase kinases 2 (MAP2K2) .
For use in combination with the LanthaScreen® ERK2 A375 cell line (K1798) to evaluate inhibitors in medium to high throughput screening applicati ...

Specifications

Readout
End Point
Reactivity
Human
Target Gene
Ser/Thr Kinases, Tyrosine Kinases
Label or Dye
Tb (Terbium)
Excitation⁄Emission (nm)
340⁄490, 520
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV5209
837a68b76082837bf35a8711c6220479_CmgtBuyProduct

ERK1+2 [pT202/pY204] Rabbit Polyclonal Antibody (Novex®)

This rabbit anti-ERK1+2 [pT202/pY204] polyclonal antibody is specific to human and rat ERK1+2 [pT202/pY204]. ERK1+2 [pT202/pY204] polyclonal antibody recognizes the expressed product of the MAPK1 gene. ERKs are regulated by the binding of extracellular ligands to transmembrane re ...

Specifications

Reactivity
Human
Rat
Validated Application
Immunohistochemistry
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ERK1/2 (pT202/pY204)
Gene ID
5594
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Affinity Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

368800
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ERK1/2 [pTpY185/187] Rabbit Polyclonal Antibody, Alexa Fluor® 488 Conjugate (Novex®)

ERK1/2 [pT185/PY187] ALEXA CONJ; Alexa Fluor 488 conjugated Polyclonal antibody specific to Human, Mouse, Rat ERK1+2 [pT185/pY187]. This antibody is validated for use in Immunocytochemistry/ Immunofluorescence. Anti-ERK1+2 [pT185/pY187] recognizes the expressed product of the MAP ...

Specifications

Reactivity
Human
Mouse
Rat
Validated Application
Immunohistochemistry
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ERK1/2 [pTpY185/187]
Gene ID
5595
Label or Dye
Alexa Fluor® 488
Excitation⁄Emission (nm)
488⁄519
Target Modification State
Phospho
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44680A1
509266fd9cc49413e008aee4c5863bc4_CmgtBuyProduct

ERK1/2 [pTpY185/187] Polyclonal Antibody, Rabbit (Novex®)

This purified Rabbit Anti-ERK 1/2 [pTpY185/187] Polyclonal Antibody is specific to human, mouse, and rat ERK 1/2 [pT185/pY187]. Anti-ERK1/2 [pT185/pY187] antibody recognizes the product of the MAPK3 gene. ERK (extracellular signal-regulated kinase), also known as MAPK (mitogen-ac ...

Specifications

Reactivity
Mouse
Human
Xenopus
Chicken
C. elegans
Drosophila
Rat
Bovine
Validated Application
Immunohistochemistry
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ERK1/2 [pTpY185/187]
Gene ID
5595
Label or Dye
Unconjugated
Target Modification State
Phospho
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44680G
79a10412807dc5fd8bdc1cdfa49f0591_CmgtBuyProduct

ERK1/2 [pTpY185/187] Antibody Pair (Novex®)

This Antibody Pair Kit comes with matched antibody pairs to detect and quantify protein levels of human phosphorylated ERK1⁄2 [pTpT185⁄187] (Extracellular signal-Regulated protein Kinase). Also available is Buffer Kit for Antibody Pairs that saves time with premade, easy-to-use ...

Specifications

Form
Concentrated
Marker
ERK1⁄2 [pTpY185⁄187]
Gene ID
5594, 5595
Platform
Microplate Reader
Reactivity
Human
Gene Symbol
MAPK3
MAPK1
Target Gene
ERK1/2 [pTpY185/187]
Label or Dye
HRP (Horseradish Peroxidase)
Emission Class
Visible
Protein Family
Kinases & Inhibitors
Target Molecule
Proteins (Phosphoproteins)
Research Category
Signal Transduction
Validated Application
ELISA
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

CHO0095
9a4b13369cc77db592a6617750369250_CmgtBuyProduct

ERK1/2 [pTpY185/187] Singleplex Bead Kit (Novex®)

This Singleplex bead kit comprises the analyte specific components for the measurement of ERK1⁄2 (Extracellular signal-Regulated protein Kinase) when phosphorylated at threonine 185 and tyrosine 187 in cell lysates or tissue homogenates. This singleplex bead kit may be multiplex ...

Specifications

Marker
ERK1⁄2 [pTpY185⁄187]
System
Luminex®
Gene ID
5595
Species
Human
Platform
Luminex® Instruments
Bead Type
Polystyrene
Bead Region
57
Gene Symbol
MAPK3
Sensitivity
0.5 U/ml
Target Gene
mitogen-activated protein kinase 3
Gene Aliases
ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, MGC20180, PRKM3, p44-ERK1, p44-MAPK
Label or Dye
R-PE
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NP_002737.2
NM_002746.2
NM_001040056.1
NP_001035145.1
NP_001103361.1
NM_001109891.1
Detection Method
Fluorescent
Research Category
Signal Transduction
Sample Type (Specific)
Tissue Homogenates
Cell Extracts
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

LHO0241
006b734b8d48810b3595905ccd25514e_CmgtBuyProduct

LanthaScreen® Tb-pATF2 (pSer71) Antibody Kit

A member of the leucine zipper family of DNA binding proteins, ATF2 (activating transcription factor 2) is phosphorylated at Thr71 by members of the JNK and p38 families of kinases. ...

Specifications

Format
384-well plate
Readout
End Point
Technique
TR-FRET
Reactivity
Human
Label or Dye
Tb (Terbium)
GFP (EmGFP)
Target Enzyme
Serine⁄Threonine Kinases
Validated Kinases
MAPK1, MAPK3, MAPK8, MAPK9, MAPK10, MAPK11, MAPK12, MAPK13, MAPK14
Antibody Molecular Weight
150000 Da
Excitation⁄Emission (nm)
340⁄490, 520
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV4452
ac5196f23f7a8f8937d6c3893d27ad37_CmgtBuyProduct

p44/42 MAPK (ERK1/2) (pT202/pY204), Oligoclonal Antibody, ABfinity™ Recombinant (Novex®)

This purified oligoclonal recombinant rabbit IgG antibody recognizes human p44/42 MAPK (ERK1/2) [pT202/pY204]. ...

Specifications

Reactivity
Human
Validated Application
Immunocytochemistry
ELISA
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Clone ID
6HCLC
Antibody Fragment
Whole Antibody
Target Gene
MAPK (ERK1/2) p44/42 (pT202/pY204)
Gene ID
5595
Gene Symbol
MAPK3
Accession Number
P27361
Gene Aliases
ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
Label or Dye
Unconjugated
Target Modification State
Phospho
Form
Liquid
Buffer
Phosphate buffered saline (PBS) with 0.09% sodium azide
Other Additives
Sodium Azide Preservative
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

710318
96912635c4e9c6f1bac1fb8bbc7df941_CmgtBuyProduct

ERK 1/2 [pTpY185/187] Monoclonal Antibody (clone 15H10L7), ABfinity™ Recombinant (Novex®)

This purified ABfinity™ recombinant rabbit anti-ERK 1/2 [pTpY185/187] monoclonal antibody is specific to human ERK 1/2 [pTpY185/187], and does not recognize non-phosphorylated ERK1/2. Based on sequence identity and similarity, reactivity to mouse, rat, chimpanzee, bovine, chicken ...

Specifications

Reactivity
Human
Validated Application
Immunofluorescence
Immunohistochemistry
Western Blot
Flow Cytometry
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
15H10L7
Antibody Fragment
Whole Antibody
Target Gene
ERK1/2 [pT185/pY187]
Gene ID
5595, 5594
Accession Number
P27361, P28482
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Affinity Purified
Concentration
0.5 mg⁄ml
Color
Unconjugated
Form
Liquid
Neutralizing Antibody
No
Platform
Flow Cytometer
Fluorescence Microscope
Confocal Microscope
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

700012
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