Genomics and proteomics search results

FRAP1 (mTOR) Recombinant Human Protein

FRAP1 (mTOR) is a serine⁄threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR⁄PI3-kinase⁄AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently ...

Specifications

Species
Human
Target Gene
FK506 binding protein 12-rapamycin associated protein 1
Gene Aliases
FRAP1 (mTOR)
Kinase Group
Ser⁄Thr Kinases: Atypical
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NP_004949.1
Molecular Weight
163.9 kDa
Research Category
Signal Transduction
Oncology
System Compatibility
LanthaScreen® Substrate
LanthaScreen® Antibody
Z'-LYTE™
Validated Application
Kinase Assay
Protein Length or Portion (a.a.)
aa 1360-2549
Protein Tag or Fusion
GST Tag
Expression
Baculovirus
Kinase Family
PIKK Kinase Family
Protein Form
Catalytic
Recombinant
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV4753
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FRAP1 (mTOR) Assay Buffer

1X Assay Buffer for use with the LanthaScreen® FRAP1 (mTOR) assays. ...

Specifications

Form
Liquid
Shipping Condition
Approved for shipment at Room Temperature or on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV4794
c0d2d898c7684d671fbb97842962c42d_CmgtBuyProduct

mTOR Mouse Monoclonal Antibody (clone 215Q18) (Novex®)

mTOR MAb (215Q18) hu, ms, rt ; Unconjugated Monoclonal antibody specific to Human, Mouse, Rat mTOR. This antibody is validated for use in Western Blot. Anti-mTOR recognizes the expressed product of the FRAP1 gene. ...

Specifications

Reactivity
Human
Mouse
Rat
Validated Application
Western Blot
Host
Mouse
Clonality
Monoclonal
Clone ID
215Q18
Target Gene
mTOR
Gene ID
2475, 56717, 56718
Label or Dye
Unconjugated
Purification
Affinity Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

AHO1232
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mTOR Monoclonal Antibody (clone 7H13L18), ABfinity™ Recombinant (Novex®)

This is a purified rabbit anti-human mTOR monoclonal antibody that can be use in the validated applications of indirect ELISA, Western blot, and immunocytochemistry. ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Western Blot
Immunocytochemistry
ELISA
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
7H13L18
Antibody Fragment
Whole Antibody
Target Gene
mechanistic target of rapamycin (serine/threonine kinase)
Gene ID
2475
Gene Symbol
MTOR
Accession Number
P42345
Gene Aliases
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Label or Dye
Unconjugated
Concentration
0.5 mg⁄ml
Form
Liquid
Protein Family
Kinases & Inhibitors
Buffer
PBS (Phosphate Buffered Saline)
Research Category
Signal Transduction
Other Additives
Sodium Azide Preservative
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

701483
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mTOR Oligoclonal Antibody, ABfinity™ Recombinant (Novex®)

This is a purified rabbit anti-human mTOR antibody that can be used in validated applications such as indirect ELISA, western blot (WB), flow cytometry (FC), and immunocytochemistry (ICC). ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Western Blot
Immunocytochemistry
ELISA
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Oligoclonal
Clone ID
7HCLC
Antibody Fragment
Whole Antibody
Target Gene
mechanistic target of rapamycin (serine/threonine kinase)
Gene ID
2475
Gene Symbol
MTOR
Accession Number
P42345
Gene Aliases
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Label or Dye
Unconjugated
Concentration
0.5 mg⁄ml
Form
Liquid
Protein Family
Kinases & Inhibitors
Buffer
PBS (Phosphate Buffered Saline)
Research Category
Cell Cycle & Proliferation
Other Additives
Sodium Azide Preservative
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

710408
ff61b063b76bce609dfea7db0b4f6cff_CmgtBuyProduct

mTOR/FRAP [pS2448] Rabbit Polyclonal Antibody (Novex®)

MTOR [pS2448] PAb; Unconjugated Polyclonal antibody specific to Human, Mouse, H, (Rat) mTOR [pS2448]. This antibody is validated for use in Western Blot, Immunocytochemistry⁄ Immunofluorescence. Anti-mTOR [pS2448] recognizes the expressed product of the FRAP1 gene. Catalog numbe ...

Specifications

Reactivity
Human
Mouse
Validated Application
Western Blot
Immunofluorescence
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
mTOR/FRAP [pS2448]
Gene ID
2475
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

441125G
5dc690482bffdb41958abb01a17111f7_CmgtBuyProduct

Rapamycin (Novex®)

Rapamycin is a member of a family of macrolide immunosuppressants that bind to and inhibit the FK506 binding protein (FKBP) proline rotamase. ...

Specifications

Form
Solid
Gene ID
2475
Gene Symbol
MTOR
Gene Aliases
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Molecular Weight
914.2 Da
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PHZ1235
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LanthaScreen® Akt HEK 293E Cell Line

LanthaScreen® GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. The PI3K⁄AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. Because of t ...

Specifications

System
LanthaScreen®
Readout
TR-FRET
Reporter
GFP (EmGFP)
Cell Line
293E (HEK)
Cell State
Dividing Cells
Antagonists
PI-103
LY294002
Gene Symbol
AKT1
Primary Agonist
IGF-1
Detection Method
Fluorescent
Druggable Target
Kinases
Signaling Pathway
Optimal Response
IGF-1 (EC50 = 0.42 µg/ml)
Alternate Agonists
Insulin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1615
e48bc13a49a899aec6f5029f8ef58ac6_CmgtBuyProduct

mTOR Antibody (Thermo Scientific™)

Western blot analysis of PA1-188 detects mTOR at ~250 kDa in human and non-human primate cells, slightly below the theoretical MW of ~280kDa. Using a Tris-Acetate SDS gel for high molecular weight protein separation, and a nitrocellulose membrane for protein transfer are necessar ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA1188
2a6e1361071c65ea9ef5a6de9ed5e4b4_CmgtBuyProduct

mTOR Antibody (Thermo Scientific™)

3-8% Tris-Acetate Gel is recommended for Western blot. PA1-518 detects a predominant band at ~290kD corresponding to mTOR. Other lower MW nonspecific bands of unknown identity were also detected at ~65kD and ~35kD in some cell lysates. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
99c36fbbe49acfd132d636bfea403504_CmgtBuyProduct

TOR Antibody (Thermo Scientific™)

A suggested positive control is L1210 cell lysate. PA5-20123 can be used with blocking peptide PEP-0241. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA520123
7f331bc28c3e25b0ed126c401a515fcd_CmgtBuyProduct

Phospho-mTOR pSer2448 Antibody (Thermo Scientific™)

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA535652
34fd4b1ef3e92e8ee76c974c9242d2b5_CmgtBuyProduct

Phospho-mTOR pSer2481 Antibody (Thermo Scientific™)

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA535651
0f27f588c3a9a1eaedc45169ad0bb2ef_CmgtBuyProduct

Phospho-mTOR pThr2446 Antibody (Thermo Scientific™)

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA535650
c8b4d1dea2525f8f01a054b6c396f2fc_CmgtBuyProduct