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GeneArt® Seamless Cloning and Assembly Kit

The GeneArt® Seamless Cloning and Assembly Kit enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction. The kit contains everything required for the assembly ...

Specifications

Maximum Size
13 kb total (vector plus all inserts)
Workflow Step
DNA Assembly
Cloning Method
Seamless Cloning
Number of Fragments
Up to 4 Fragments
Bacterial or Yeast Strain
TOP10
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13288
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GeneArt® Site-Directed Mutagenesis System

The GeneArt® Site-Directed Mutagenesis System provides a state-of-the-art, simple, convenient, and highly efficient means to generate mutations on a target construct in vitro in less than three hours. This system replaces the popular GeneTailor™ Site-Directed Mutagenesis System, ...

Specifications

Mutagenesis Capability
Single-Site Mutagenesis
Plasmid Type
Plasmids up to 14 kb
Mutation Type
Substitutions
Insertions
Deletions
Mutagenesis Efficiency
90%
Bacterial or Yeast Strain
DH5α™
Mutagenized Region Length (up to)
12 nucleotides
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13282
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GeneArt® High-Order Genetic Assembly System

The GeneArt® High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and recombine DNA fragments with high ...

Specifications

Maximum Size
110 kb total (vector plus all inserts)
Workflow Step
DNA Assembly
Cloning Method
Seamless Cloning
Number of Fragments
Up to 10 Fragments
Bacterial or Yeast Strain
MaV203
TOP10
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13285
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GeneArt® Chlamydomonas Engineering Kit

The GeneArt® Chlamydomonas Engineering Kit is the first commercially available genetic modification and expression system for the eukaryotic microalgae, Chlamydomonas reinhardtii. The GeneArt® Chlamydomonas Engineering Kit combines optimized cloning and expression vectors, frozen ...

Specifications

Promoter
Hsp70A-RbcS2
Cell Line
Chlamydomonas
Expression
Algae
Key Function
Algal Engineering
Delivery Method
Transformation
Protein Tag or Fusion
Untagged
Selection Agent (Eukaryotic)
Hygromycin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14258
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GeneArt® High-Order Genetic Assembly Systems, with yeast growth media

The GeneArt® High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and recombine DNA fragments with high ...

Specifications

Maximum Size
110 kb total (vector plus all inserts)
Workflow Step
DNA Assembly
Cloning Method
Seamless Cloning
Number of Fragments
Up to 10 Fragments
Bacterial or Yeast Strain
MaV203
TOP10
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13286
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GeneArt® Chlamydomonas TOPO® Engineering Kit

The GeneArt® Chlamydomonas TOPO® Engineering Kit is the first commercially available genetic modification and expression system for the eukaryotic microalgae, Chlamydomonas reinhardtii. The GeneArt® Chlamydomonas TOPO® Engineering Kit combines TOPO® cloning and expression vectors ...

Specifications

Promoter
Hsp70A-RbcS2
Cell Line
Chlamydomonas
Expression
Algae
Key Function
Algal Engineering
Delivery Method
Transformation
Protein Tag or Fusion
Untagged
Selection Agent (Eukaryotic)
Hygromycin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14260
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GeneArt® Chlamydomonas TOPO® Engineering Kit, with 6 L media

The GeneArt® Chlamydomonas TOPO® Engineering Kit is the first commercially available genetic modification and expression system for the eukaryotic microalgae, Chlamydomonas reinhardtii. The GeneArt® Chlamydomonas TOPO® Engineering Kit with media combines TOPO® cloning and express ...

Specifications

Promoter
Hsp70A-RbcS2
Cell Line
Chlamydomonas
Expression
Algae
Key Function
Algal Engineering
Delivery Method
Transformation
Protein Tag or Fusion
Untagged
Selection Agent (Eukaryotic)
Hygromycin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14264
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GeneArt® Service Cloning into Gateway® pLenti6.3/V5-DEST™ (Ready-to-Work Package)

The GeneArt® Gene Synthesis service offers chemical synthesis, cloning, and sequence verification of virtually any desired genetic sequence. ...

Specifications

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GeneArt® Seamless Cloning and Assembly Enzyme Mix

GeneArt® Seamless Cloning and Assembly Enzyme Mix enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction. GeneArt® Seamless enzyme mix is the economical cho ...

Specifications

Maximum Size
13 kb total (vector plus all inserts)
Workflow Step
DNA Assembly
Cloning Method
Seamless Cloning
Number of Fragments
Up to 4 Fragments
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14606
1ddbea816e2e32eb2e615a38a57d9d74_CmgtBuyProduct

GeneArt® Site-Directed Mutagenesis PLUS System

The GeneArt® Site-Directed Mutagenesis PLUS System brings our mutagenesis technology to the next level with the ability to perform single- or multi-site mutations in larger plasmids with greater efficiency and in less time than the competition. ...

Specifications

Mutagenesis Capability
Mutagenesis at Multiple Sites
Plasmid Type
Plasmids up to 14 kb
Mutation Type
Substitutions
Insertions
Deletions
Mutagenesis Efficiency
90 %
Bacterial or Yeast Strain
DH5α™
Mutagenized Region Length (up to)
3 nucleotides (multi-site)
25 nucleotides (single-site)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14604
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GeneArt® Linear pUC19L Vector for Seamless Cloning

The GeneArt® Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt® Seamless Cloning and Assembly Kit (cat # A13288). The vector is ma ...

Specifications

Reporter
Beta-Gal (lacZ)
Structure
Linear
Vector Type
Seamless Cloning Vectors
Maximum Size
13 kb total (vector plus all inserts)
Cloning Method
Seamless Cloning
Replication Origin
pUC Origin
Number of Fragments
Up to 4 Fragments
Antibiotic Resistance (Bacterial)
Ampicillin (AmpR)
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13289
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