CellSensor® NFkB-bla ME-180 Cell Line

The CellSensor® NFκB-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the Nuclear Factor Kappa B (NFκB) response element stably integrated into ME-180 cells. The cell line was created through fluorescence activated cell sorting (FACS) of cells respons ...

Specifications

System
CellSensor™
Cell State
Dividing Cells
Druggable Target
Signaling Pathway
Cytokines
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Fluorescein-IKK Substrate

A fluorescein-labeled peptide substrate that is primarily used with the IkappaB kinase (IKK) family. ...

Specifications

Gene ID
1147, 2869, 3551
Substrate
Fluorescein-IKK
Label or Dye
FITC (Fluorescein)
Target Enzyme
Serine⁄Threonine Kinases
Substrate Type
Kinase Substrate
Detection Method
Fluorescent
Molecular Weight
1864 Da
Validated Kinases
CHUK, GRK5, GRK6, IKBKB, IKBKE
Amino Acid Sequence
Fluorescein- DRHDSGLDSMKDE
Substrate Properties
Peptide-Based Substrate
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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CHUK (IKK α) Recombinant Human Protein

CHUK (IKK alpha) is a serine⁄threonine kinase involved in signal transduction and cellular communication. It belongs to the cytokine activated protein complex that is an inhibitor of the NF-kappa B complex. ...

Specifications

Gene ID
114294, 1147
Species
Human
Target Gene
conserved helix-loop-helix ubiquitous kinase
Gene Aliases
CHUK (IKK α)
Kinase Group
Ser⁄Thr Kinases: Other
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NP_001269
Molecular Weight
88.7 kDa
Research Category
Signal Transduction
System Compatibility
Adapta™
LanthaScreen® Substrate
LanthaScreen® Antibody
Validated Application
Kinase Assay
Protein Tag or Fusion
His Tag
Expression
Baculovirus
Kinase Family
IKK Kinase Family
Protein Form
Full Length
Recombinant
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Adapta™ Universal Kinase Assay Kit

The Adapta™ Universal Kinase Assay Kit is a homogenous, fluorescence-based immunoassay for the detection of ADP produced by kinases or other ATP dependent enzymes. ...

Specifications

Format
384-well plate
Gene ID
1022, 1147, 120892, 1612, 3376, 5289, 5290, 5294, 8536
Readout
End Point
Technique
TR-FRET
Assay Type
Universal Kinase Assay
Label or Dye
Europium
Alexa Fluor® 647
Target Enzyme
Kinases (Multiple)
Validated Kinases
CAMK1, CDK7, CDK9, CHUK, LRRK2, PI4KB, PIK3C2B, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PIK3C2A
Excitation⁄Emission (nm)
340⁄615, 665
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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LanthaScreen® Tb-IKBa pSer32 Antibody Kit

An essential component of the canonical NF-kB pathway, phosphorylation of IkappaBalpha at Ser32 by IKK preceedes the transactivation of NF-kappaB. ...

Specifications

Format
384-well plate
Gene ID
1147, 2869, 3551
Readout
End Point
Technique
TR-FRET
Reactivity
Human
Label or Dye
FITC (Fluorescein)
Tb (Terbium)
Target Enzyme
Serine⁄Threonine Kinases
Validated Kinases
CHUK, GRK5, GRK6, IKBKB, IKBKE
Antibody Molecular Weight
150000 Da
Excitation⁄Emission (nm)
340⁄490, 520
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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IKK-alpha [pSpS176/180] Polyclonal Antibody, Rabbit

This rabbit anti-IKK-alpha [pSpS176/180] polyclonal antibody is specific to human IKK-alpha [pSpS176/180] and expected to react with mouse (not tested). IKK-alpha [pSpS176/180] polyclonal antibody recognizes the expressed product of the CHUK gene. IκB Kinase-α (IKKα) is an 85 kDa ...

Specifications

Reactivity
Human
Validated Application
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
IKKalpha [pSpS176/180]
Gene ID
1147
Label or Dye
Unconjugated
Target Modification State
Phospho
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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