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New quick method for isolating RNA from laser captured cells stained by immunofluorescent immunohistochemistry; RNA suitable for direct use in fluorogenic TaqMan one-step real-time RT-PCR.

Citations & References

  • Authors: Gallup JM, Kawashima K, Lucero G, Ackermann MR,
  • Journal: Biol Proced Online (2005) 7:70-92
  • Product Usage: Products were used to purify RNA from laser captured cells retrieved from frozen sections previously subjected to immunofluorescent immunohistochemistry (IF-IHC). RNA was used in one-step real-time quantitative RT-PCR. This avoids the need for the need for costly, time-consuming linear amplification.
  • ID: PN66273
Catalog #s
18580008
AmplificationGradeDNaseI
ARCTURUSXT
portal4331182
SuperScriptIIIRT
UltrapureGlycogen
466fb9bd9d76e8388d327709c005d02e_Citations

Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer.

Citations & References

  • Authors: Kube DM, Savci-Heijink CD, Lamblin AF, Kosari F, Vasmatzis G, Cheville JC, Connelly DP, Klee GG,
  • Journal: BMC Mol Biol (2007) 8:25-25
  • Product Usage: First-strand cDNA synthesis was performed using SuperScript™ II or III (Invitrogen, Carlsbad, CA) and oligo(dT)12–18 (Invitrogen). One µL of LCM RNA was used per 20 µL cDNA synthesis reaction. Typically, 0.25 µL of the resulting cDNA reaction was used per qPCR reaction, and samples were analyzed in triplicate.
  • ID: PN66676
Catalog #s
18580400
SuperScriptIII
e26ebd564a492f55c8ceed4d97c5fedb_Citations

Assessment of fixatives, fixation, and tissue processing on morphology and RNA integrity.

Citations & References

  • Authors: Cox ML, Schray CL, Luster CN, Stewart ZS, Korytko PJ, M Khan KN, Paulauskis JD, Dunstan RW,
  • Journal: Exp Mol Pathol (2006) 80:183-191
  • Product Usage: Laser capture microdissection and Taqman qRT-PCR was done on LCM that were collected followed by RNA isolation using the RNAqueous-Micro kit including DNase treatment. Samples were quantified by UV spectrophotometry, and 5 ng of each was reverse transcribed using the SuperScript™ III Platinum® Two-step qRT-PCR Kit in 10 µl, including 5 ng cDNA for each sample. Predesigned Taqman primer/probe sets for eukaryotic 18S rRNA, PPIA, cyclophilin A, HPRT
  • ID: PN67586
Catalog #s
RNAqueousMicrokit
SuperScriptIIIPlatinumTwostepTaqManAssays18sPPIAHPRTCycloph
8c09ef2da7c91a5a9dcd27d03a573bb7_Citations

Analysis of one-step and two-step real-time RT-PCR using SuperScript III.

Citations & References

  • Authors: Wacker MJ, Godard MP,
  • Journal: J Biomol Tech (2005) 16:266-271
  • Product Usage: The real-time RT-PCR reactions were carried out using SuperScript III Platinum One-Step Quantitative RT-PCR System and SuperScript III Platinum Two-Step Quantitative RT-PCR Kit.
  • ID: PN66452
Catalog #s
11728081
11728089
11745100
11745500
11747100
11747500
1b0396cb5231cfa3b6977db0b4381616_Citations

Analysis of one-step and two-step real-time RT-PCR using SuperScript III.

Citations & References

  • Authors: Wacker MJ; Godard MP
  • Journal: Journal of Biomolecular Techniques : Jbt 2006 3:266-271
  • Product Usage: AmpliTaq Gold® Reagents
  • ID: PN93338
Catalog #s
4318739
4327058
4327059
64af0b65bb3371cf1f19d5607adca704_Citations

SuperScript Indirect cDNA Labeling System for microarrays

Citations & References

  • Authors: Weidong Zheng, Pauline Lieu, Anne-Laure, Wizman, Sergio Gurrieri, Patrick Gilles, Jun Lee
  • Journal: FOCUS (2003) 25:16-19
  • Product Usage: The SuperScript™ III Indirect cDNA Labeling System produces the highest cDNA yield using thermal stable SuperScript™ III RT and a mixture of AAdUTP and AH-dATP. The mixture of amino-modified dNTPs results in efficient coupling of fluorescent dye to cDNA. Microarray analysis demonstrates that the SuperScript™ Indirect Labeling System yields hybridization signals with greater intensity and higher signal-to-noise ratios than the commonly utilized competitive indirect labeling methods.
  • ID: PN63835
Catalog #s
L101401
L101402
0a09c8844ba8f0936c20bd791130d6b6_Citations

Localization of the transglutaminase cross-linking site in SVS III, a novel glycoprotein secreted from mouse seminal vesicle.

Citations & References

  • Authors: Lin Han-Jia; Luo Ching-Wei; Chen Yee-Hsiung;
  • Journal: J Biol Chem (2002) 277:3632-3641
  • Product Usage: First-strand cDNA synthesis was achieved by Superscript II Reverse Transcriptase.
  • ID: PN63247
Catalog #s
18064022
fe6f3afebbe889231eac68c1ba5f8815_Citations

Quantitative two-step RT-PCR analysis using LUX Primers and reverse transcriptases stored at various temperatures

Citations & References

  • Authors: Taurai Nenguke, Donald Selway, Anjali Redkar
  • Journal: FOCUS (2003) 25:33-35
  • Product Usage: Micro-to-midi RNA purification kit was used to extract total RNA from HeLa cells. SuperScript II and III were used to obtain the cDNAs. Real-time PCR was performed using Platinum® Quantitative PCR SuperMix-UDG with FAM-labeled LUX™ TFRC (Transferrin Receptor) primer.
  • ID: PN60410
Catalog #s
111H02
11730017
12183018
12313011
18064105
18080044
e00da03b685a0dd18fb6a08af0923de0_Citations

Methods for gene silencing with RNAi.

Citations & References

  • Authors: March JC, Bentley WE,
  • Journal: Methods Mol Biol (2007) 388:427-434
  • Product Usage:
  • ID: PN133716
Catalog #s
AM1914
5264c5131ed263cccd244e4cbd05b41c_Citations

High throughput immuno-screening of cDNA expression libraries produced by in vitro recombination; exploring the Plasmodium falciparum proteome.

Citations & References

  • Authors: Kordai Sowa MP, Sharling L, Humphreys G, Cavanagh DR, Gregory WF, Fenn K, Creasey AM, Arnot DE,
  • Journal: Mol Biochem Parasitol (2004) 133:267-274
  • Product Usage: Total RNA was isolated from the SD2H3/5-CSA parasite using TRIzol. The first strand was synthesized with oligo-dT and SuperScript II (part of GeneRacer kit. Superscript II has been replaced with SuperScript III). The second strand was synthesized with PCR. pDONR221 and pBAD-DEST49 were used to create the entry clone and the expression clone, respectively. Transformation of electro-competent TOP10 was done in a high-throughput fashion. Expression was induced with arabinose.
  • ID: PN63649
Catalog #s
11789013
11791019
12283016
15596018
18249029
C404050
L150201
f5e647292cc4e1064968ca62bebe7e47_Citations

Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR.

Citations & References

  • Authors: Levesque-Sergerie JP, Duquette M, Thibault C, Delbecchi L, Bissonnette N,
  • Journal: BMC Mol Biol (2007) 8:93-93
  • Product Usage: RNA samples used as background material for the RT assays were prepared (serial dilution from the same RNA pool; see above) in aliquots of 10, 25, 50,100, 1,000, and 2,000 ng total testis RNA. All the RT systems screened in this study, i.e. Sensiscript and Omniscript (Qiagen), SuperScript™ II and SuperScript™ III (Invitrogen), and PowerScript™ (Clontech, Mountain View, CA), were used strictly in accordance with the recommendations of the respective suppliers.
  • ID: PN66675
Catalog #s
18064014
18064022
18064071
3337a3c31ebcc50989297a8cf95c3720_Citations

Improved full-length cDNA production based on RNA tagging by T4 DNA ligase.

Citations & References

  • Authors: Clepet C, Le Clainche I, Caboche M,
  • Journal: Nucleic Acids Res (2004) 32:e6-e6
  • Product Usage: Trizol was used to isolate total RNA from 4-week-old A.thaliana aerial vegetative tissues. SuperScript III was used to convert the RNA into cDNA in a reaction of 50 min at 48oC. cDNA was later amplified with PCR and inserted into Gateway pDONR221 to create the entry clone.
  • ID: PN66006
Catalog #s
11789013
12535019
15596018
18080044
43975bc2dfc84641a2a8c4d3fe653176_Citations

Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner.

Citations & References

  • Authors: Tsuchihara K, Lapin V, Bakal C, Okada H, Brown L, Hirota-Tsuchihara M, Zaugg K, Ho A, Itie-Youten A, Harris-Brandts M, Rottapel R, Richardson CD, Benchimol S, Mak TW,
  • Journal: Cancer Res (2005) 65:6685-6691
  • Product Usage: cDNA was generated from 1 x 104 mouse embryonic stem cells using Superscript III CellsDirect cDNA Synthesis Kit.
  • ID: PN63868
Catalog #s
11737030
34f98c7c5d7063181da890ea8d25265a_Citations

Interactive effects of neurohypophyseal neuropeptides with receptor antagonists on passive avoidance behavior: mediation by a cerebral neurohypophyseal hormone receptor?

Citations & References

  • Authors: de Wied D, Elands J, Kovács G,
  • Journal: Proc Natl Acad Sci U S A (1991) 88:1494-1498
  • Product Usage: SuperScript™ III was used to synthesize first-strand cDNA for one hour at 50°C. First-strand cDNA was incubated with T7-oligo(dT) primer for three minutes at 65°C in preparation for second-strand cDNA synthesis. After cleanup of double-stranded cDNA, 0.5 µL was saved for qPCR analysis, and the remaining cDNA was used to synthesize biotin-labeled cRNA.
  • ID: PN66446
Catalog #s
18080119
18080127
18080135
18080143
18080400
c4efc214b3e14109e4619773eb3fd469_Citations

DNA vaccination with CCL2 DNA modified by the addition of an adjuvant epitope protects against

Citations & References

  • Authors: Zheng G, Wang Y, Xiang SH, Tay YC, Wu H, Watson D, Coombes J, Rangan GK, Alexander SI, Harris DC,
  • Journal: J Am Soc Nephrol (2006) 17:465-474
  • Product Usage: The researchers used the SuperScript III Platinum CellsDirect kit to investigated whether introduction of a foreign T helper epitope into monocyte chemoattractant protein 1 (CCL2) DNA vaccine could boost its immunogenicity.
  • ID: PN62687
Catalog #s
11737030
1e00746ce7635c403c2d4f9767893f00_Citations