MAX Efficiency® DH5α™ Competent Cells

MAX Efficiency® DH5α™ Competent Cells are a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ cells increase insert stability and improve the quality of plasmid D ...

Specifications

Competent Cell Type
Chemically Competent
Species
E. coli
Format
Tube(s)
Transformation Efficiency
> 1x10^9
Bacterial or Yeast Strain
DH5α™
Blue-White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Reduces Recombination
Yes
Cloning Methylated DNA
No
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
F' Episome (to make ssDNA)
Lacks F' Episome
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA)
No
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Subcloning Efficiency™ DH5α™ Competent Cells

Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or ...

Specifications

Competent Cell Type
Chemically Competent
Species
E. coli
Format
Tube(s)
Transformation Efficiency
> 1x10^6
Bacterial or Yeast Strain
DH5α™
Blue-White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Reduces Recombination
Yes
Cloning Methylated DNA
No
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
F' Episome (to make ssDNA)
Lacks F' Episome
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA)
No
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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One Shot® MAX Efficiency® DH5α™-T1R Competent Cells

One Shot® MAX Efficiency® DH5α™-T1R Competent Cells are derived from the popular DH5α™ strain. DH5α™-T1R carry the tonA genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library con ...

Specifications

Competent Cell Type
Chemically Competent
Species
E. coli
Format
One Shot
Transformation Efficiency
> 1x10^9
Bacterial or Yeast Strain
DH5α™
Blue-White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Reduces Recombination
Yes
Cloning Methylated DNA
No
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
F' Episome (to make ssDNA)
Lacks F' Episome
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA)
Yes
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Library Efficiency® DH5α™ Competent Cells

Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 108 cfu/µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application t ...

Specifications

Competent Cell Type
Chemically Competent
Species
E. coli
Format
Tube(s)
Transformation Efficiency
> 1x10^8
Bacterial or Yeast Strain
DH5α™
Blue-White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Reduces Recombination
Yes
Cloning Methylated DNA
No
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
F' Episome (to make ssDNA)
Lacks F' Episome
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA)
No
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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ElectroMAX™ DH5α-E™ Competent Cells

ElectroMAX™ DH5α-E™ Competent Cells are derived from the DH5α™ strain and are suitable for transformation by electroporation. They may be used in procedures requiring high transformation efficiencies, such as generation of cDNA libraries or in transformations with limited input D ...

Specifications

Competent Cell Type
Electrocompetent
Species
E. coli
Format
Tube(s)
Transformation Efficiency
> 1x10^10
Bacterial or Yeast Strain
DH5α™
Plasmid Type
May be used for plasmids > 20 kb
Blue-White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Reduces Recombination
Yes
Cloning Methylated DNA
Yes
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
F' Episome (to make ssDNA)
Lacks F' Episome
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA)
No
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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MAX Efficiency® DH5α™-T1R Competent Cells

MAX Efficiency® DH5α™ T1 Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ increase insert stability and improve the quality of plasmid D ...

Specifications

Competent Cell Type
Chemically Competent
Species
E. coli
Format
Tube(s)
Transformation Efficiency
> 1x10^9
Bacterial or Yeast Strain
DH5α™
Blue-White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Reduces Recombination
Yes
Cloning Methylated DNA
No
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
F' Episome (to make ssDNA)
Lacks F' Episome
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA)
Yes
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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MAX Efficiency® DH5αF´IQ™ Competent Cells

MAX Efficiency® DH5αF´IQ™ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.
MAX Effici ...

Specifications

Competent Cell Type
Chemically Competent
Species
E. coli
Format
Tube(s)
Transformation Efficiency
> 1x10^8
Bacterial or Yeast Strain
DH5α™
Blue-White Screening
Yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Reduces Recombination
Yes
Cloning Methylated DNA
No
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
F' Episome (to make ssDNA)
Contains F' Episome
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA)
No
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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TOPO® TA Cloning® Kit for Sequencing, with One Shot® MAX Efficiency® DH5α-T1R E. coli

The TOPO® TA Cloning® Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing. Each kit uses the pCR™4-TOPO® TA vector with special ...

Specifications

Vector Type
TOPO-TA Cloning Vectors
Cloning Method
TOPO®-TA
Bacterial or Yeast Strain
DH5α™
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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TOPO® TA Cloning® Kit, Dual Promoter, with One Shot® MAX Efficiency™ DH5α-T1R E. coli

TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR®-TOPO® Vectors include: ...

Specifications

Vector Type
TOPO-TA Cloning Vectors
Cloning Method
TOPO®-TA
Bacterial or Yeast Strain
DH5α™
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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TOPO® TA Cloning® Kit, with One Shot® MAX Efficiency™ DH5α-T1R E. coli

TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR®-TOPO® Vectors include: ...

Specifications

Vector Type
TOPO-TA Cloning Vectors
Cloning Method
TOPO®-TA
Bacterial or Yeast Strain
DH5α™
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Zero Blunt® TOPO® PCR Cloning Kit for Sequencing, with One Shot® MAX Efficiency™ DH5α-T1R E. coli

The Zero Blunt® TOPO® PCR Cloning Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing. Each kit uses the pCR ...

Specifications

Vector Type
Blunt DNA Cloning Vectors
Cloning Method
Blunt TOPO®
Bacterial or Yeast Strain
DH5α™
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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Zero Blunt® TOPO® PCR Cloning Kit, with One Shot® MAX Efficiency DH5α-T1R E. coli

Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR®-Blunt II-TOPO® Vector for 5-minute ...

Specifications

Vector Type
Blunt DNA Cloning Vectors
Cloning Method
Blunt TOPO®
Bacterial or Yeast Strain
DH5α™
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K282020
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