Plasmid pBR322 (Invitrogen™)

Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli, also has 14 non-selectable unique restriction endonuclease recognition si ...

Specifications

Vector Type
pBR322
Cloning Method
Restriction Enzyme ⁄ MCS
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

15367014
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pBR322 Plasmid DNA (Thermo Scientific™)

Thermo Scientific pBR322 is one of the most commonly used E.coli cloning vectors. ...

Specifications

Vector Type
pBR322
Cloning Method
Restriction Enzyme ⁄ MCS
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SD0041
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pBR322 DNA/BsuRI (HaeIII) Marker (Thermo Scientific™)

Thermo Scientific pBR322 DNA/BsuRI (HaeIII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. pBR322 DNA is digested to completion with the appropriate Thermo Scientific ...

Specifications

Gel Compatibility
Agarose Gels
Ready to Load
No
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SM0271
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pUC19 DNA (Thermo Scientific™)

Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation. ...

Specifications

Vector Type
pUC19
Cloning Method
Restriction Enzyme ⁄ MCS
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SD0061
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pUC18 DNA (Thermo Scientific™)

Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation. ...

Specifications

Vector Type
pUC18
Cloning Method
Restriction Enzyme ⁄ MCS
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SD0051
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SgeI (3 U/µL) (Thermo Scientific™)

Thermo Scientific SgeI restriction enzyme recognizes m5CNNG(9/13)^ sites and cuts best at 37°C in its own unique buffer. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of ...

Specifications

Enzyme
SgeI
Compatible Buffer
Unique Buffer (10x Buffer SgeI)
Methylation Sensitivity
Not CpG methylation-sensitive
Not dcm methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER2211
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CseI (HgaI) (5 U/µL) (Thermo Scientific™)

Thermo Scientific CseI (HgaI) restriction enzyme recognizes GACGC(5/10)^ sites and cuts best at 37°C in R buffer (isoschizomers: HgaI). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of ...

Specifications

Enzyme
CseI (HgaI)
Compatible Buffer
10x Buffer R
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER1901
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Lsp1109I (BbvI) (5 U/µL) (Thermo Scientific™)

Thermo Scientific Lsp1109I (BbvI) restriction enzyme recognizes GCAGC(8/12)^ sites and cuts best at 37°C in its own unique buffer (isoschizomers: BbvI, BstV1I). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, op ...

Specifications

Enzyme
Lsp1109I (BbvI)
Compatible Buffer
Unique Buffer (10x Buffer Lsp1109I (BbvI))
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
Not CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER2071
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BseXI (BbvI) (3 U/µL) (Thermo Scientific™)

Thermo Scientific BseXI (BbvI) restriction enzyme recognizes GCAGC(8/12)^ sites and cuts best at 65°C in its own unique buffer (isoschizomers: BbvI, BstV1I). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optim ...

Specifications

Enzyme
BseXI (BbvI)
Compatible Buffer
Unique Buffer (10x Buffer BseXI (BbvI))
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
Not CpG methylation-sensitive
Optimal Reaction Temperature
65° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER1452
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EheI (SfoI) (10 U/µL) (Thermo Scientific™)

Thermo Scientific EheI (SfoI) restriction enzyme recognizes GGC^GCC sites and cuts best at 37°C in Tango buffer. Isoschizomers: BbeI, DinI, EgeI, KasI, Mly113I, NarI, SfoI. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction ...

Specifications

Enzyme
EheI (SfoI)
Compatible Buffer
10x Buffer Tango
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER0441
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SalI (10 U/µL) (Thermo Scientific™)

Thermo Scientific SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer Sy ...

Specifications

Enzyme
Sal I
Compatible Buffer
10x Buffer O
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER0641
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NheI (10 U/µL) (Thermo Scientific™)

Thermo Scientific NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (isoschizomers: AsuNHI, BmtI). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of ...

Specifications

Enzyme
Nhe I
Compatible Buffer
10x Buffer Tango
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER0972
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DpnI (10 U/µL) (Thermo Scientific™)

Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buf ...

Specifications

Enzyme
Dpn I
Compatible Buffer
10x Buffer Tango
Methylation Sensitivity
Not CpG methylation-sensitive
dam methylation-sensitive
Not dcm methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER1705
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EcoRII (10 U/µL) (Thermo Scientific™)

Thermo Scientific EcoRII restriction enzyme recognizes ^CCWGG sites and cuts best at 37°C in O buffer. Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzym ...

Specifications

Enzyme
EcoRII
Compatible Buffer
10x Buffer O
Methylation Sensitivity
Not dam methylation-sensitive
dcm methylation-sensitive
Not CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER1921
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