• Catalog
    [0]
  • Learn More
    [0]
  • Documents & Support
    [3]

Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation.

Citations & References

  • Authors: Day RC, Herridge RP, Ambrose BA, Macknight RC,
  • Journal: Plant Physiol (2008) 148:1964-1984
  • Product Usage: Arabidopsis cDNA was generated using the VILO cDNA synthesis kit and Real-time qRT-PCR done using Express SYBR GreenER MasterMix in 7-uL volumes on a LightCycler 480. Differential expression using arrays and qRT-PCR: Quantification using the Ribogreen RNA quantification kit. First round aRNA made using Message Amp II kit wass primed with random hexamers to make first-strand cDNA using SuperScript III and used for qRT-PCR. Second round IVT was done to generate target for microarray study.
  • ID: PN67584
Catalog #s
11754050
1178401K
MessageAmpIIAmbionAM1751
R11490
76ca7dbd5eae268ed3fb7e3caccb979d_Citations

A useful approach to total analysis of RISC-associated RNA.

Citations & References

  • Authors: Hayashida Y, Nishibu T, Inoue K, Kurokawa T,
  • Journal: BMC Res Notes (2009) 2:169-169
  • Product Usage: Isolation of RISC-associated mRNAs: Isolation of small RNA fraction (_ 200nt) using mirVana miRNA Kit from HeLa, HEK293, HepG2, THP-1 and P388D1. HepG2 cells were transfected with 600pmol miR-122 siRNA and a control using Lipofectamine RNAiMax. Dynal ProteinG beads bound with 5µg of anti-human antibody was used in immunoprecipitation of RNA protein complexes. SuperScript VILO cDNA Synthesis Kit and 2×Power SYBR Master Mix were used for Quantitative-RT-PCR on the ABI 7500.
  • ID: PN67583
Catalog #s
10003D
11754050
13778075
A11193050
MirVanaAmbionAM1560
PowerSybrAppliedBiosystems4368
fb15e2247f302f53eff1d2cd472c7711_Citations

Cyclin D1 splice variants: polymorphism, risk, and isoform-specific regulation in prostate cancer.

Citations & References

  • Authors: Comstock CE, Augello MA, Benito RP, Karch J, Tran TH, Utama FE, Tindall EA, Wang Y, Burd CJ, Groh EM, Hoang HN, Giles GG, Severi G, Hayes VM, Henderson BE, Le Marchand L, Kolonel LN, Haiman CA, Baffa R, Gomella LG, Knudsen ES, Rui H, Henshall SM, Sutherland RL, Knudsen KE,
  • Journal: Clin Cancer Res (2009) 15:5338-5349
  • Product Usage: RNA was extracted form PCa cell lines using TRIzol. qRT-PCR was done on SuperScript II generated cDNA on a StepOne Instrument using either Taqman assays or Express SYBR Green system. Also, Human prostate tissue was OCT-embedded and incubated in RNAlater-ICE. After OCT removal, tissue was minced and homogenized in Trizol to obtain RNA and DNA for PCR-RFLP. Radiolabeled cDNA was also generated using SuperScript VILO
  • ID: PN67582
Catalog #s
11754050
1178401K
15596018
18064105
4331182
4351368
4351370
4351372
RNAlaterICE4427575
StepOneInstrument4376357
TaqManAssay4331348
3e8654a245b8c843da72f29ed36bc1f1_Citations