Synthesis of a cleavable dinucleotide photoaffinity probe of ribonucleic acid polymerase: application to trinucleotide labeling of an Escherichia coli transcription complex.

Citations & References

  • Authors: Hanna MM, Meares CF
  • Journal: Biochemistry (1983) 22:3546-3551
  • Product Usage:
  • ID: PN1914
Catalog #s
43900cc8434685fe6937a619d4246be5_Citations

Spin-labeling studies of aminoacyl transfer ribonucleic acid.

Citations & References

  • Authors: Schofield P, Hoffman BM, Rich A
  • Journal: Biochemistry (1970) 9:2525-2533
  • Product Usage:
  • ID: PN1367
Catalog #s
S520
6a45c2b39692eedbfd0b378c47a5a693_Citations

Fluorescent affinity labeling of initiation site on ribonucleic acid polymerase of Escherichia coli.

Citations & References

  • Authors: Wu FY, Wu CW
  • Journal: Biochemistry (1974) 13:2562-2566
  • Product Usage:
  • ID: PN70
Catalog #s
I14
4f67f486f9c069b4529a73c3f98642fe_Citations

Protocol for 5′ End Labeling RNA | Life Technologies

Experiment Protocol

0a6ef42172ee84706b9bf3fda492f264_iProtocol

Protocol for 3′ End Labeling RNA | Life Technologies

Experiment Protocol

c028bf9d5386a4af44925cebfd63af68_iProtocol

Can I use the NCode™ miRNA labeling system with plant RNA?

Product FAQ

Answer

We have not tested plant RNA directly with our NCode™ miRNA labeling system. However, plant miRNA has been successfully polyadenylated and used for first-strand cDNA synthesis and subsequent qPCR. Therefore, it may be possible to label plant miRNA with this kit.

Answer Id: 5046

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8cea559c47e4fbdb73b23e0223d04e79_FAQ

Can I use the SuperScript® Indirect cDNA Labeling System for bacterial samples?

Product FAQ

Answer

We have not used bacterial RNA for the validation of the SuperScript® Indirect cDNA Labeling System, however the kit should work reasonably well with few modifications to the protocol.

For total RNA samples, Oligo(dT)20 primers should be used. As the manual suggests, random hexamers should be used only with mRNA or with the RNA ladder, which is included as a control. However, in the case of bacterial RNA, longer random primers should be used instead of the random hexamers included in the kit. 18mers should work well, although the temperature of reverse transcription may need to be slightly adjusted in order to obtain optimal cDNA synthesis. The reason for using longer random primers is that the rRNA in bacterial samples will also be used as template for reverse transcription, and the target cDNA will be more complex than the corresponding cDNA primed with the Oligo(dT)20. Therefore, when doing hybridizations, you can expect some degree of cross-hybridization to occur. Nevertheless, the use of long random 18mers is common practice among microarray users.

Answer Id: 4147

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Indirect cDNA Labeling for Microarrays | Life Technologies

Experiment Protocol

75e607492c78f751fa9409b7a6086d8c_iProtocol

How do you get labeled probe from the SuperScript® RNA Amplification System?

Product FAQ

Answer

This kit converts your RNA into amplified amounts of in vitro transcribed RNA. There are two labeling options to chose from: (1) include labeled UTP in the in vitro transcription to result in labeled RNA probe or (2) perform a first-strand synthesis on the non-labeled, in vitro transcribed RNA in the presence of labeled dCTP or dTTP to result in labeled cDNA probe.

Answer Id: 4917

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cf8d8c66b1212720e569b0bd67695451_FAQ

Can long dsRNAs be labeled with your Silencer® siRNA Labeling Kits? What about single stranded RNA?  Can DNA be labeled as well?

Product FAQ

Answer

Long dsRNAs can be labeled; please refer to the website ordering pages for AM1632 and AM1634 to find a link to a supplemental protocol.  

ssRNA and ssDNA/dsDNA of varying lengths can also be labeled with these kits.  But note that although shorter oligonucleotides have been successfully labeled (our scientists have tried oligos as short as 10 nt), the base:dye ratio or % labeled will be lower with decreasing size.

Answer Id: 2878

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User Guide: Arcturus Paradise PLUS Reagent System-FFPE Tissue Staining, Extraction, Isolation, Amplification, Microarray Labeling

Manual / Product Insert

  • Download
  • Version: 12872-00 Rev. D 07.2010
Catalog #s
KIT0103
KIT0312J
1efc1b615012fad9d233b3f3ca069dd1_Manuals

SuperScript™ Indirect cDNA Labeling System | Life Technologies

Experiment Protocol

9aa051298404ffdc80f920ee37ccd630_iProtocol

Kits for Labeling Proteins and Nucleic Acids—Section 1.2 | Life Technologies

Molecular Probes Handbook

5d975f14378f13c01f78e7cdfa352343_ProbesHandbook

Application Note: Labeling exosomal RNA and membrane components using flourescent dyes

Product Literature

2b323d6eb28422cef49b266557dd31ad_Brochures

Manual: UB Subject: Biotin Labeling of Oligonucleotides on a DNA/RNA Synthesizer (English )

Manual / Product Insert

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bb8054b490d695da02c573c665824b88_Manuals