Can KnockOut™ SR be used to culture mouse ESCs on feeder cells?

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Yes, KnockOut™ SR was designed to culture mouse ES cells in undifferentiated conditions.

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Can KnockOut™ SR be used to culture mouse ESCs in feeder-free conditions?

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Yes, mouse embryonic stem cells can be cultured using KnockOut™ SR under feeder-free conditions. Typically, cells are plated at a higher seeding density, in the presence of LIF, on a 0.1% gelatin layer in place of feeder cells.

Answer Id: 4977

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