What is the size of the CAT protein when expressed in bacteria?

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Excluding any amino acids resulting from fusion tags, CAT has an apparent molecular weight of 28-32 kD on SDS-PAGE gels.

Answer Id: 3242

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What is the source of the EM7 promoter?

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The EM7 promoter is a synthetic promoter derived from the T7 promoter. The promoter is typically used to drive expression of antibiotic resistance genes for selection in E. coli.

Answer Id: 3270

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Where is the transcriptional start site of the T7 promoter featured in many of Invitrogen™'s vectors?

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Although Invitrogen™ has not formally mapped the transcriptional start site of the T7 promoter, the following reference indicates that the start site occurs at the G following the CACTATA sequence found in the promoter: Nucleic Acids Research, Vol.20, No. 20, pp 4626-4634.

Answer Id: 4435

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