What is the size of the CAT protein when expressed in bacteria?

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Answer

Excluding any amino acids resulting from fusion tags, CAT has an apparent molecular weight of 28-32 kD on SDS-PAGE gels.

Answer Id: E3242

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2181fd7ddcd8781522cb2d232c328b0c_FAQ

What is the source of the EM7 promoter?

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Answer

The EM7 promoter is a synthetic promoter derived from the T7 promoter. The promoter is typically used to drive expression of antibiotic resistance genes for selection in E. coli.

Answer Id: E3270

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f0b4e94eac48c91516111407482622af_FAQ

Where is the transcriptional start site of the T7 promoter featured in many of Invitrogen™'s vectors?

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Answer

Although Invitrogen™ has not formally mapped the transcriptional start site of the T7 promoter, the following reference indicates that the start site occurs at the G following the CACTATA sequence found in the promoter: Nucleic Acids Research, Vol.20, No. 20, pp 4626-4634.

Answer Id: E4435

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dc73c49ce03192d2f93e6d0a7e333e45_FAQ

Do you offer Gateway® vectors for expression in plants?

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Answer

We do not offer any Gateway® vectors for expression in plants.

Answer Id: E9854

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f35a2a2fda93397e37ba9cec45315b52_FAQ

Can I purchase the 5X LR Clonase® buffer or 5X BP Clonase® buffer separately?

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Answer

We do not offer the 5X LR Clonase® buffer and 5X BP Clonase® buffer as standalone products. They are available as part of the enzyme kits.

Answer Id: E9855

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0dd65ff7d2983c8ab717067f11025f11_FAQ

How can I move my gene of interest from a Gateway®-adapted expression clone to a new Destination vector as I have lost the entry clone?

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Answer

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Answer Id: E9856

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919b5529a674f6d2956edaddb7a15612_FAQ

Do you have a recommended single-step protocol for BP/LR recombination?

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Answer

Yes, we have come up with a single-step protocol for BP/LR Clonase® reaction (http://www.lifetechnologies.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

Answer Id: E9857

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80619757f4a4dae3ad303e4aa46318b5_FAQ

Can I perform the single-step protocol for the BP/LR Clonase® reaction using BP Clonase® enzyme and LR Clonase® enzyme instead of BP Clonase® II enzyme and LR Clonase® II enzyme?

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Answer

In the single-step protocol for the BP/LR Clonase® reaction, we would not recommend substituting the BP Clonase® II/LR Clonase® II enzymes with BP Clonase® /LR Clonase® enzymes as this would result in very low recombination efficiency.

Answer Id: E9858

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40248bad60307808063848f3acac9030_FAQ