Do you have a non-animal-origin enzyme for cell dissociation?

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Answer

Yes, the TrypLE™ products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE™ enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum supplemented systems. The catalog numbers for TrypLE™ Select are 12563-011 (100mL) and 12563-029 (500mL). Some example catalog numbers for TrypLE™ Express include 12604-013 without Phenol red (100 ml) and 12605-010 with Phenol red (100 ml) - both formulations are also available in additional sizes. All these products are animal origin free.

Answer Id: E4277

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What is the difference between TrypLE™ Express and TrypLE™ Select?

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Answer

Both of the products contain rProtease, non-animal, trypsin-like enzyme formulations used for the dissociation of attachment dependent cell lines. This alternative for porcine Trypsin is a recombinant enzyme derived from microbial fermentation.

The Express and Select products differ in their production and testing methods and in the documentation provided. TrypLE™ Select is for the industrial market and has release testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF dedicated equipment and has Intended Use for Research Use/Further Cell Culture Manufacturing.

TrypLE™ Express is manufactured without use of dedicated equipment and is intended for Research Use Only, Not intended for human or animal diagnostic or therapeutic use.

Answer Id: E5567

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