Which serum-free media can be used to culture human keratinocytes?
There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (catalog # 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (catalog #10744-019) or Epilife® medium (catalog # M-EPI-500-CA) supplemented with EDGS (catalog # S-012-5). If you would like an animal origin free defined system, Epilife® medium (catalog # M-EPI-500-CA) with S7 supplement (catalog # S-017-5) is an excellent choice.
Answer Id: 4740
Which serum-free media can be used to culture mouse keratinocytes?
Are the Keratinocyte SFM basal medium and supplement kit available separately?
The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.
Answer Id: 4742