In the TaqMan® GAPDH Control Reagents Kit (Cat. No. 402869), what is the size of the GAPDH amplicon?

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Answer

The amplicon length is 226 bases, and is based on the NCBI GenBank® accession number J04038. More information on the GAPDH amplicon can be found in Appendix B of the “TaqMan® Gold RT-PCR Kit” protocol that is found on the Product Detail Page for Cat. No. 402869. Enter the catalog number in the main Search field on our web site to view this page and the Protocol.

Answer Id: 1561

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Do your human and rodent GAPDH primers and TaqMan® probe sequences detect genomic DNA?

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Answer

It varies by product, so be sure to check all the available product specifications before purchase. Here are details on a few options:

- The TaqMan® GAPDH Control Reagents (Human), Cat. No. 402869, which is labeled with JOE™ dye, spans two exons therefore will not amplify genomic DNA.

- The Human GAPD (GAPDH) Endogenous Control (VIC®/ TAMRA™ Probe, Primer Limited), Cat. No. 4310884E, is also designed across an exon-exon boundary and will not amplify genomic DNA.

- The probe in the TaqMan® Rodent GAPDH Control Reagents kit, Cat. No. 4308313, labeled with VIC® dye, does not span an intron and therefore may amplify genomic DNA.

Answer Id: 1435

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1f3202d820180a39f736f20fce790de8_FAQ

Do the human and rodent GAPDH primers and TaqMan® probe sequences manufactured by Applied Biosystems® detect genomic DNA?

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Answer

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- Taqman® GAPDH Control Reagents, human, 402869, JOE™: Does not detect genomic DNA
- Taqman® Endogenous Control, GAPDH for human, 4326317E, VIC® : Does not detect genomic DNA
- Taqman® Endogenous Control, GAPDH, human, 4310884E, VIC®: Does not detect genomic DNA
- Taqman® Endogenous Control, GAPDH, human, 4333764F, FAM™: Does not detect genomic DNA
- Taqman® Endogenous Control, GAPDH, human, 4333764T, FAM™: Does not detect genomic DNA
- Taqman® Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM™: Does not detect genomic DNA

Answer Id: 1517

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Where can I find a protocol describing the use of the TaqMan® human GAPDH Control Reagents (Cat. No. 402869)?

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Answer

The protocol for the use of the TaqMan® Human GAPDH Control Reagents is provided within the protocol for the TaqMan® Gold RT-PCR Kit. A copy of the document can be found on the product page for Catalog No. 402869.

Answer Id: 1369

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