I am working on both mouse and human samples for gene expression studies. Do you have one endogenous control that I can use with both species?

Product FAQ

Answer

The 18S rRNA is highly conserved among eukaryotes. We have several configurations of assays for this gene: P/N 4331182, Assay ID: Hs99999901_s1; PN 4319413E, 4310893E, 4333760T, 4333760F, 4308329. You can use any of these for both human and mouse samples, as well as samples from any other eukaryotic species.

Answer Id: 2483

Provide Feedback on this Answer

Ask a New Question

2c6ae45a3e88aee548c0714fad7f8269_FAQ

When using the TaqMan® Gene Signature Plates, should I mix the reactions after adding the master mix and template to the wells containing lyophilized primers and probe?

Product FAQ

Answer

No, but it is recommended that the plate be centrifuged to draw down the liquid to the bottom of the wells.

Answer Id: 1370

Provide Feedback on this Answer

Ask a New Question

d1ee59e20ad01cedc15f5118a7626099_FAQ

What are the key features of a TaqMan® MGB probe?

Product FAQ

Answer

TaqMan® MGB probes have a nonfluorescent quencher at the 3´ end and a minor groove binder at the 3´ end that increases the melting temperature (Tm) of probe, allowing the use of shorter probes.  For more information on MGB probes, please consult the following manual: "Primer Express v1.5 and TaqMan® MGB Probes for Allelic Discrimination: All PCR Instruments: User Bulletin." You can find a copy on our website by entering this title in the main search box.

Answer Id: 1371

Provide Feedback on this Answer

Ask a New Question

81ca0262c82e712e50c580c032d99b60_FAQ