What are the storage conditions for the TaqMan® and Custom TaqMan® Gene Expression and SNP Genotyping Assays?

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Answer

After shipment, the assay mixes should be stored at -15 C to -20 C to maximize stability. Freeze/thaws should be kept to a minimum (a maximum of 10 freeze-thaw cycles is recommended). Keep all TaqMan® and Custom TaqMan® Gene Expression and SNP Genotyping Assays protected from direct exposure to light. Excessive exposure to light may affect the fluorescent probes. If desired, the Assay Mix may be diluted with TE buffer or DNase-free Water. It is recommended to make working stocks from the stock solutions provided in these kits.

Answer Id: E2008

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01c012371f770f15ec1d25e0ec8aebcd_FAQ

How much cDNA is recommended per TaqMan® or Custom TaqMan® Gene Expression reaction?

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Answer

For optimal performance of the TaqMan® and Custom TaqMan® Gene Expression Assays, use 1 to 100 ng of cDNA per 50 ul total reaction volume. For more information, please consult the TaqMan® Gene Expression Assay protocol and Custom TaqMan® Gene Expression Assay protocol.

Answer Id: E2012

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e56924eb60d44a090765c944bc0e9373_FAQ

When preparing the TaqMan® or Custom TaqMan® Gene Expression Assays, what is the recommended reaction volume?

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Answer

When preparing the TaqMan® or Custom TaqMan® Gene Expression Assays, the recommended and supported volume is 50ul for a 96-well standard plate set-up; 20ul for a 96-well fast plate set-up and 10 ul for a 384-well set-up. For more information, please consult the TaqMan® Gene Expression Assays Custom TaqMan® Gene Expression Assays protocols.

Answer Id: E2014

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fe5859f0e502ad3b5c10e3e92dc599e4_FAQ

Which TaqMan® assay types can be used when using the with TaqMan® Fast Universal PCR Master Mix (P/N 4352042)?

Product FAQ

Answer

The following research assays are supported using the TaqMan® Fast Universal and/or Advanced Fast PCR Master Mix: TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, Custom primers and probes designed from Primer Express® software-designed quantitative assays.

Answer Id: E2322

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0f705dc2e5b481cf79f2ebc73fcd0c8e_FAQ

What is the lowest copy number that can be used with the TaqMan® Fast Universal PCR Master Mix (P/N 4352042)?

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Answer

The fast master mix has demonstrated detection down to 10 copies of the RNase P gene with genomic DNA. Of course, detection limits may vary depending on factors such as assay design and sample preparation.

Answer Id: E2325

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e769a1f1c64861a21c47e7bf63ab15da_FAQ

What component in the TaqMan® Fast Universal PCR Master Mix or (P/N 4352042) allows rapid amplification results?

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Answer

The hot-start DNA polymerase enzyme system in the TaqMan® Fast Universal PCR Master Mix does not require an activation step, and can be activated significantly shorter hold times for the thermal cycling stages of PCR, thereby minimizing PCR run time. For more information on using this reagent, please consult the TaqMan® Fast Universal PCR Master Mix (2X) protocol.

Answer Id: E2345

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0cb3fbb4f22b4a7bb297db25b82cba25_FAQ

Can I use TaqMan® Fast Universal PCR Master Mix for both one-step and two-step RT-PCR?

Product FAQ

Answer

TaqMan® Fast Universal PCR Master Mix is recommended for two-step RT-PCR, but for one-step reactions we recommend using TaqMan® Fast Virus 1-Step Master Mix as the preferred reagent.

Answer Id: E2346

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786cd596af0af6585fa9343afd0f182d_FAQ

Is there a TaqMan® PCR Core Reagents kit for the Fast Real-Time PCR System?

Product FAQ

Answer

No. The only Fast reagents that we offer are the TaqMan® Fast Universal PCR Master Mix (2X) for single plex use, and Fast Advanced Master Mix for greater sensitivity and multiplexing use.

Answer Id: E2348

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93914bd8da7cecb26754314cbb537023_FAQ

What is the recommended input amount of template for use with the TaqMan® Fast Universal PCR Master Mix?

Product FAQ

Answer

We recommend adding 10 to 100 ng of RNA converted to cDNA as your template.

Answer Id: E2349

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9910754afab81d290a05fc58509c1741_FAQ

What are the storage conditions and the shelf life of the TaqMan® Fast Universal PCR Master Mix?

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Answer

We recommend a storage temperature of 4C and the shelf life is 9 months from the manufacturing date.

Answer Id: E2350

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5bed891e458db9fd75e5b1efb23509ee_FAQ

Can I compare performance of assays between the TaqMan® Fast Universal PCR Master Mix compare and TaqMan® Universal PCR Master Mix?

Product FAQ

Answer

In general, assays have been found to perform comparably with these reagents, but they may not provide exactly identical results.

Answer Id: E2351

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38e6ace6c82c724fcaca91958040a856_FAQ

Is the TaqMan® Fast Universal PCR Master Mix offered with AmpErase® UNG?

Product FAQ

Answer

The TaqMan® Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpErase®UNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan® Fast Advanced Master Mix.

Answer Id: E2352

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ac1b0f5d88972229e8bb667fb98822e1_FAQ

Can I run a reaction made with the standard TaqMan® Universal PCR Master Mix in fast thermal cycling mode?

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Answer

No, we do not recommend this approach. Since the standard TaqMan® Universal PCR Master Mix is not optimized for running with fast thermal cycling, assay results will be significantly compromised.

Answer Id: E2353

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bf29c579612c923edfb678750318f765_FAQ

Can I set up and store the TaqMan® Fast Universal PCR Master Mix at room temperature if the instrument is in use?

Product FAQ

Answer

For optimal results, we recommend to run the reaction plate right after setting up the reaction. If a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on the fast PCR instrument platform.

Answer Id: E2355

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b70f93e35ba13ab57def67c43b409321_FAQ

What is the difference between TaqMan® Gene Expression Assays and the Custom TaqMan® Gene Expression Assays?

Product FAQ

Answer

TaqMan® Gene Expression Assays are off-the-shelf, quantitative, human gene expression assays based on the TaqMan® probe-based chemistry. The assays are formulated into a 20X mix, QC tested and functionally tested.

The Custom TaqMan® Gene Expression Assays are designed based on the target sequences submitted by the customer, then synthesized, formulated, and delivered with analytical quality control. The target sequence information can be submitted by the customer through online Custom Assay Design Tool. The integrity of the sequence information must be verified before the design process. When you submit a sequence, you have an option to let us run the bioinformatic evaluation of the target sequence to verify the integrity. You can also run the bioinformatic evaluation by yourself. This integrity verification can be accomplished through BLAST analysis or other methods of verifying sequence integrity.

Answer Id: E1840

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032fbec5f4de4bfacec2fab8cc0eb69e_FAQ