What is a spectral calibration?

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A spectral calibration is an algorithm applied to raw data, which converts it into the component 4 or 5 dye data stored in the sample files. A spectral is created for a specific dye set (combination of dyes), array type (4 or 16 capillaries), and array length (36cm or 50cm). It is used to correct for the natural overlap of the fluorescent dyes.

Answer Id: E2530

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Which fragment analysis matrix standards can I use for my Applied Biosystems® 3130 Series instrument?

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DS-02 (Dye Set E5), DS-30 (Dye Set D), DS-31 (Dye Set D), DS-32 (Dye Set F), and DS-33 (Dye Set G5) are all supported on the Applied Biosystems® 3130 Series systems. Please refer to the Applied Biosystems® 3130/xl Genetic Analyzers Getting Started Guide for more information. (Cat. No. 4352715).

Answer Id: E2366

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