What is a spectral calibration?

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A spectral calibration is an algorithm applied to raw data, which converts it into the component 4 or 5 dye data stored in the sample files. A spectral is created for a specific dye set (combination of dyes), array type (4 or 16 capillaries), and array length (36cm or 50cm). It is used to correct for the natural overlap of the fluorescent dyes.

Answer Id: E2530

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1a474edb5c1704c56bad952b2237bbdf_FAQ

Which fragment analysis matrix standards can I use for my Applied Biosystems® 3130 Series instrument?

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DS-02 (Dye Set E5), DS-30 (Dye Set D), DS-31 (Dye Set D), DS-32 (Dye Set F), and DS-33 (Dye Set G5) are all supported on the Applied Biosystems® 3130 Series systems. Please refer to the Applied Biosystems® 3130/xl Genetic Analyzers Getting Started Guide for more information. (Cat. No. 4352715).

Answer Id: E2366

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ad3d2ec846d47c2885a688f20b96fdc1_FAQ

When should I run a spectral calibration on the Applied Biosystems® 3730 Series DNA Analyzer?

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A new spectral calibration should be run:

- Whenever a new dye set is used on an instrument.
- After any optics adjustment performed by a service engineer.
- If the instrument has been moved.
- If you see a decrease in spectral separation (pull-up and/or pull-down peaks).
- If you alter any conditions (dye set, array type, or array length).
- For G5-RCT, Any4dye-HDR, and S dye set users only: Any time the array detection cell is moved/repositioned.

Answer Id: E2531

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50255cef6002e8dec711100e5453727d_FAQ

How often should a spectral calibration be run on the Applied Biosystems® 3730 Series DNA Analyzer?

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There is no fixed time as to when a spectral must be run. However, some users prefer to run a new spectral at a preset time to ensure it is up to date. Data quality will be the largest indicator of when a new spectral is required. A separate spectral calibration needs to be performed for each dye set, array type, and array length. For G5-RCT dye set users only: a spectral calibration must be re-run if the array detection cell is moved/repositioned. Refer to FAQ "When should I run a spectral calibration?" for more information on when a spectral should be run.

Answer Id: E2532

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