Which of your plastic consumables are compatible with the Applied Biosystems® 7900HT Fast Real-Time PCR System?

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Answer

There are many of our reagents and plastic consumables that can be used on the Applied Biosystems® 7900HT Fast Real-Time PCR System. Here are a few examples to help you get started:

Cat. No. 4313663, MicroAmp® Optical Adhesive Film Kit
Cat. No. 4311971, 4360954, MicroAmp® Optical Adhesive Film
Cat. No. 4323032, MicroAmp® Optical 8-Cap Strip
Cat. No. 4309849; 4326270, MicroAmp® Optical 384-Well Reaction Plate with Barcode
Cat. No. 4306737; 4326659; 4314320, MicroAmp® Optical 96-Well Reaction Plate with Barcode
Cat. No. 4346906, MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL
Cat. No. 4312063, Splash Free 96-Well Base

For more comprehensive lists of compatible plastics, enter "Real-Time PCR Plastics" in the main Search field on our website and follow the links.

Answer Id: 1596

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309fee4e541e51de2e41f21bebb342aa_FAQ

Are the MicroAmp™ Optical 96-Well Reaction Plates and MicroAmp™ Optical 384 -Well Reaction Plates autoclavable?

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Answer

Our microplates are certified to be DNA, RNase and PCR inhibitor free as long as they are kept in the original packaging bag. They are not meant to be autoclaved.

Answer Id: 2500

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f7696a9b362ac5a51c3dc8f098b73923_FAQ

Can I place an Optical Fast 96-Well plate onto a standard Applied Biosystems® 7500 or 7300 Real-Time PCR System?

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No. The fast plate will not fit correctly into the standard block and assay performance will be significantly compromised.

Answer Id: 2357

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Is the Applied Biosystems® 7900HT SDS Automation Controller software and Zymark Twister® Instrument compatible with MicroAmp® Fast Optical 96-Well Reaction Plates with Barcode (P/N 4346906)?

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Answer

No. The current version of the Zymark Twister® Instrument and SDS Automation Controller software is NOT compatible with MicroAmp® Fast 96-Well Optical Plates with Barcode. The Zymark Twister® Instrument is compatible with MicroAmp® Optical 96-Well Reaction Plate with Barcode, MicroAmp® Optical 384-Well Reaction Plate with Barcode, as well as TaqMan® Low Density Arrays.

Answer Id: 2509

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8db9264228dc48fbf47535e888c02ae0_FAQ

What is the total reaction volume that I can be load into the Optical 96-Well Fast Thermal Cycling Plate (P/N 4346906)?

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Answer

The minimal reaction volume is 10 ul to 30 ul in the Optical 96-Well Fast Thermal Cycling Plate.

Answer Id: 2324

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d5e2fbef30a4eb668a203060ec8e5eef_FAQ

What are the differences between the Applied Biosystems® 7500 Fast Real-Time PCR System and the standard 7500 Real-Time PCR System?

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Answer

The 7500 Fast Real-Time PCR System contains a new high-speed thermal cycling block designed specifically to work with Optical 96-well Fast thermal cycling plates (Cat. No. 4346906) and TaqMan® Fast Universal PCR Master Mix (Cat. No. 4352042). It also has a new precision plate holder design, for use only with Optical 96-well Fast thermal cycling plates, and looks slightly different with a front bezel and a darker tray drawer color.

Answer Id: 2311

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046ddf96c233a273fd390c3d0b1a9aa4_FAQ

How can I run a standard TaqMan® assay reaction on the Applied Biosystems® 7500 Fast Real-Time PCR System?

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Answer

You can run an Applied Biosystems® 7500 Fast Real-Time PCR System instrument in Standard mode as long the following set-up points are followed:

1) The reaction volume is between 10 ul-30 ul
2) The 96-well plate is a MicroAmp® FAST 96 well reaction plate (P/N 4346907, 4346906 or 4366932)
3) Select the Run Mode to be “Standard 7500 “or “9600 Emulation” (2.0.x software versions only offer Standard or Fast - no 9600 emulation choice).

Answer Id: 2474

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0bf727e907c5fc9d5356f11e4c45d613_FAQ

What is the recommended minimum and maximum volume that can be used on any Applied Biosystems® Real-Time PCR Instrument?

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Answer

The reaction volume per well is depends on what type of plate of a particular instrument that you use. The answer is provided by the information below based on the plate type.

96-well standard plate (Part Numbers: N8010560, 4316813, 4306737 and 4326659) Recommend volume: 50ul; Maximum volume: 100ul; Minimum volume: 25ul

96-well fast plate (Part Numbers: 4346907, 4346906 or 4366932) Recommend volume: 20ul; Maximum volume: 30ul; Minimum volume: 10ul

48-well fast plate (Part Numbers: 4375816) Recommend volume: 20ul; Maximum volume: 30ul; Minimum volume: 10ul

384-well plate (Part Numbers: 4309849, 4326720, 4343814 and 4343370) Recommended volume: 10ul; Maximum volume: 30ul; Minimum volume: 5ul

Answer Id: 2742

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