Which of your plastic consumables are compatible with the Applied Biosystems® 7900HT Fast Real-Time PCR System?

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Answer

There are many of our reagents and plastic consumables that can be used on the Applied Biosystems® 7900HT Fast Real-Time PCR System. Here are a few examples to help you get started:

Cat. No. 4313663, MicroAmp® Optical Adhesive Film Kit
Cat. No. 4311971, 4360954, MicroAmp® Optical Adhesive Film
Cat. No. 4323032, MicroAmp® Optical 8-Cap Strip
Cat. No. 4309849; 4326270, MicroAmp® Optical 384-Well Reaction Plate with Barcode
Cat. No. 4306737; 4326659; 4314320, MicroAmp® Optical 96-Well Reaction Plate with Barcode
Cat. No. 4346906, MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL
Cat. No. 4312063, Splash Free 96-Well Base

For more comprehensive lists of compatible plastics, enter "Real-Time PCR Plastics" in the main Search field on our website and follow the links.

Answer Id: 1596

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309fee4e541e51de2e41f21bebb342aa_FAQ

Can I place an Optical Fast 96-Well plate onto a standard Applied Biosystems® 7500 or 7300 Real-Time PCR System?

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No. The fast plate will not fit correctly into the standard block and assay performance will be significantly compromised.

Answer Id: 2357

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d095a94d20dcaf7aa07301948549bede_FAQ

Are the MicroAmp™ Optical 96-Well Reaction Plates and MicroAmp™ Optical 384 -Well Reaction Plates autoclavable?

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The MicroAmp™ Optical 96-Well Reaction Plates and MicroAmp™ Optical 384 -Well Reaction Plates are autoclavable.

Answer Id: 2500

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What is the total reaction volume that I can be load into the Optical 96-Well Fast Thermal Cycling Plate (P/N 4346906)?

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Answer

The minimal reaction volume is 10 ul to 30 ul in the Optical 96-Well Fast Thermal Cycling Plate.

Answer Id: 2324

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Is the Applied Biosystems® 7900HT SDS Automation Controller software and Zymark Twister® Instrument compatible with MicroAmp® Fast Optical 96-Well Reaction Plates with Barcode (P/N 4346906)?

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Answer

No. The current version of the Zymark Twister® Instrument and SDS Automation Controller software is NOT compatible with MicroAmp® Fast 96-Well Optical Plates with Barcode. The Zymark Twister® Instrument is compatible with MicroAmp® Optical 96-Well Reaction Plate with Barcode, MicroAmp® Optical 384-Well Reaction Plate with Barcode, as well as TaqMan® Low Density Arrays.

Answer Id: 2509

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8db9264228dc48fbf47535e888c02ae0_FAQ

What are the differences between the Applied Biosystems® 7500 Fast Real-Time PCR System and the standard 7500 Real-Time PCR System?

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The 7500 Fast Real-Time PCR System contains a new high-speed thermal cycling block designed specifically to work with Optical 96-well Fast thermal cycling plates (Cat. No. 4346906) and TaqMan® Fast Universal PCR Master Mix (Cat. No. 4352042). It also has a new precision plate holder design, for use only with Optical 96-well Fast thermal cycling plates, and looks slightly different with a front bezel and a darker tray drawer color.

Answer Id: 2311

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046ddf96c233a273fd390c3d0b1a9aa4_FAQ

How can I run a standard TaqMan® assay reaction on the Applied Biosystems® 7500 Fast Real-Time PCR System?

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Answer

You can run an Applied Biosystems® 7500 Fast Real-Time PCR System instrument in Standard mode as long the following set-up points are followed:

1) The reaction volume is between 10 ul-30 ul
2) The 96-well plate is a MicroAmp® FAST 96 well reaction plate (P/N 4346907, 4346906 or 4366932)
3) Select the Run Mode to be “Standard 7500 “or “9600 Emulation” (2.0.x software versions only offer Standard or Fast - no 9600 emulation choice).

Answer Id: 2474

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0bf727e907c5fc9d5356f11e4c45d613_FAQ

What is the recommended minimum and maximum volume that can be used on any Applied Biosystems® Real-Time PCR Instrument?

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The reaction volume per well is depends on what type of plate of a particular instrument that you use. The answer is provided by the information below based on the plate type.

96-well standard plate (Part Numbers: N8010560, 4316813, 4306737 and 4326659) Recommend volume: 50ul; Maximum volume: 100ul; Minimum volume: 25ul

96-well fast plate (Part Numbers: 4346907, 4346906 or 4366932) Recommend volume: 20ul; Maximum volume: 30ul; Minimum volume: 10ul

48-well fast plate (Part Numbers: 4375816) Recommend volume: 20ul; Maximum volume: 30ul; Minimum volume: 10ul

384-well plate (Part Numbers: 4309849, 4326720, 4343814 and 4343370) Recommended volume: 10ul; Maximum volume: 30ul; Minimum volume: 5ul

Answer Id: 2742

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