Can I use any reverse transcription kit for a TaqMan® MicroRNA RT reaction?

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No, only the TaqMan® MicroRNA Reverse Transcription Kit (Cat. No. 4366596 (200 reactions)) or Cat. No. 4366597 (1,000 reactions)) should be used. This kit was developed specifically for use with TaqMan® MicroRNA Assay reverse transcription.

Answer Id: 2768

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What is mechanism of the TaqMan® MicroRNA Assays?

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Answer

There are 2 steps involved in using the TaqMan® MicroRNA Assays:

1. RT step: total RNA to cDNA using miRNA-specific RT primers from the TaqMan® MicroRNA Assays and the TaqMan® MicroRNA Reverse Transcription Kit (P/N 4366596, 200 reactions or 4366597, 1000 reactions)

2. PCR step: cDNA to PCR products using primers and probe from the TaqMan MicroRNA Assays together with TaqMan® Universal PCR Master Mix No AmpErase® UNG (P/N 4324018).

Answer Id: 2516

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