What TaqMan® Copy Number Assays are available now?

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Answer

We have predesigned TaqMan® Copy number assays for human and mouse, and we also have the Geneassist Copy number assay workflow builder tool that you may use to submit sequences for custom designed assays. Copy number variation experiments require a reference assay run in duplex with the target assay. We offer RNase P and TERT reference assays for human, and we offer Tfrc and TERT reference assays for mouse. The copy number assays are FAM-MGB labeled and the copy number reference assays are VIC-TAMRA labeled.

Answer Id: E2728

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e1ec71c187ca4819a579c381d20c9fe9_FAQ

What endogenous control is recommended for TaqMan® Copy Number Assays?

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Answer

We recommend that you run one of our TaqMan® Copy Number Reference assays in duplex with your target copy number assay. In human, we offer Rnase P and TERT copy number reference assays; in mouse, we offer Tfrc and TERT copy number reference assays. The reference assays are VIC-TAMRA labeled so that they can be run in duplex with your target copy number assay of interest.

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53b2227689f3dd87ab03abde1d2a66c7_FAQ

Where can I get reference samples for TaqMan® Copy Number Assays?

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Answer

We recommend that you check the DGV (database of genomic variants) database for your CNV of interest. Not all regions of variance have control samples available, but if there are controls for your CNV, the DGV will list some Coriell sample ids. You can then order these control samples from Coriell.

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5000fdec292ec23d000289401d0ce21d_FAQ

Do I need to do a validation test before I use the ΔΔCt method to determine the copy number of the target gene?

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Answer

If you are using our copy number assays and reference assays, this is not necessary.

Answer Id: E2734

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708c9fb44c4138ab0fcbbd5125eee6cc_FAQ

What type of samples should be run with TaqMan® Copy Number Assays?

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Answer

These assays target regions of variance that are found in genomic DNA.

Answer Id: E2735

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Can I use TaqMan® Gene Expression Assays for a gene copy number study?

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Answer

TaqMan® Gene Expression Assays with “_s1” as assay ID suffix are the assays whose primers and probes are designed within a single exon, and therefore, will detect genomic DNA. However, the application of these assays in gene copy number studies has not been validated. Other gene expression assays will not detect gDNA, let alone the region of variance.

Answer Id: E2736

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