What are the storage conditions for the TaqMan® and Custom TaqMan® Gene Expression and SNP Genotyping Assays?

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Answer

After shipment, the assay mixes should be stored at -15 C to -20 C to maximize stability. Freeze/thaws should be kept to a minimum (a maximum of 10 freeze-thaw cycles is recommended). Keep all TaqMan® and Custom TaqMan® Gene Expression and SNP Genotyping Assays protected from direct exposure to light. Excessive exposure to light may affect the fluorescent probes. If desired, the Assay Mix may be diluted with TE buffer or DNase-free Water. It is recommended to make working stocks from the stock solutions provided in these kits.

Answer Id: 2008

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ef8446f35513a8d6aa2308357a268a7e_FAQ

How much cDNA is recommended per TaqMan® or Custom TaqMan® Gene Expression reaction?

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Answer

For optimal performance of the TaqMan® and Custom TaqMan® Gene Expression Assays, use 1 to 100 ng of cDNA per 50 ul total reaction volume. For more information, please consult the TaqMan® Gene Expression Assay protocol and Custom TaqMan® Gene Expression Assay protocol.

Answer Id: 2012

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253614bbac999b38b5b60cae531c4969_FAQ

When preparing the TaqMan® or Custom TaqMan® Gene Expression Assays, what is the recommended reaction volume?

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Answer

When preparing the TaqMan® or Custom TaqMan® Gene Expression Assays, the recommended and supported volume is 50ul for a 96-well standard plate set-up; 20ul for a 96-well fast plate set-up and 10 ul for a 384-well set-up. For more information, please consult the TaqMan® Gene Expression Assays Custom TaqMan® Gene Expression Assays protocols.

Answer Id: 2014

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cee8d6b7ce52554fd70354e37bbf44a2_FAQ

Which TaqMan® assay types can be used when using the with TaqMan® Fast Universal PCR Master Mix (P/N 4352042)?

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Answer

The following research assays are supported using the TaqMan® Fast Universal and/or Advanced Fast PCR Master Mix: TaqMan® Gene Expression Assays, Custom TaqMan® Gene Expression Assays, Custom primers and probes designed from Primer Express® software-designed quantitative assays.

Answer Id: 2322

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228bbc2f87caeb21bb7f6949fddcb91d_FAQ

What is the lowest copy number that can be used with the TaqMan® Fast Universal PCR Master Mix (P/N 4352042)?

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Answer

The fast master mix has demonstrated detection down to 10 copies of the RNase P gene with genomic DNA. Of course, detection limits may vary depending on factors such as assay design and sample preparation.

Answer Id: 2325

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6c97cd07663b099253bc569fe8d342bb_FAQ

What component in the TaqMan® Fast Universal PCR Master Mix or (P/N 4352042) allows rapid amplification results?

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The hot-start DNA polymerase enzyme system in the TaqMan® Fast Universal PCR Master Mix does not require an activation step, and can be activated significantly shorter hold times for the thermal cycling stages of PCR, thereby minimizing PCR run time. For more information on using this reagent, please consult the TaqMan® Fast Universal PCR Master Mix (2X) protocol.

Answer Id: 2345

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81b073de9370ea873f548e31b8adc081_FAQ

Can I use TaqMan® Fast Universal PCR Master Mix for both one-step and two-step RT-PCR?

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Answer

TaqMan® Fast Universal PCR Master Mix is recommended for two-step RT-PCR, but for one-step reactions we recommend using TaqMan® Fast Virus 1-Step Master Mix as the preferred reagent.

Answer Id: 2346

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777669af68dbccabc30c3b6bcaa81825_FAQ

Is there a TaqMan® PCR Core Reagents kit for the Fast Real-Time PCR System?

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No. The only Fast reagents that we offer are the TaqMan® Fast Universal PCR Master Mix (2X) for single plex use, and Fast Advanced Master Mix for greater sensitivity and multiplexing use.

Answer Id: 2348

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20c9f5700da1088260df60fcc5df2b53_FAQ

What is the recommended input amount of template for use with the TaqMan® Fast Universal PCR Master Mix?

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We recommend adding 10 to 100 ng of RNA converted to cDNA as your template.

Answer Id: 2349

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70fcb77e6349f4467edd7227baa73222_FAQ

What are the storage conditions and the shelf life of the TaqMan® Fast Universal PCR Master Mix?

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We recommend a storage temperature of 4C and the shelf life is 9 months from the manufacturing date.

Answer Id: 2350

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326a8c055c0d04f5b06544665d8bb3ea_FAQ

Can I compare performance of assays between the TaqMan® Fast Universal PCR Master Mix compare and TaqMan® Universal PCR Master Mix?

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Answer

In general, assays have been found to perform comparably with these reagents, but they may not provide exactly identical results.

Answer Id: 2351

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0abdc563a06105aee3c6136871c9f4d1_FAQ

Is the TaqMan® Fast Universal PCR Master Mix offered with AmpErase® UNG?

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Answer

The TaqMan® Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpErase®UNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan® Fast Advanced Master Mix.

Answer Id: 2352

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92049debbe566ca5782a3045cf300a3c_FAQ

Can I run a reaction made with the standard TaqMan® Universal PCR Master Mix in fast thermal cycling mode?

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Answer

No, we do not recommend this approach. Since the standard TaqMan® Universal PCR Master Mix is not optimized for running with fast thermal cycling, assay results will be significantly compromised.

Answer Id: 2353

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23af4b45f1e166141a790d1a3126e77a_FAQ

Can I set up and store the TaqMan® Fast Universal PCR Master Mix at room temperature if the instrument is in use?

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Answer

For optimal results, we recommend to run the reaction plate right after setting up the reaction. If a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on the fast PCR instrument platform.

Answer Id: 2355

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