What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems®?

Product FAQ

Answer

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express® software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

Answer Id: E1359

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3f005b88b12109bdc2b339c56f1f58fd_FAQ

What computer configurations can I use with the ViiA™ 7 Fast Real-Time PCR System?

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Answer

The current recommended configuration is the Dell Gx980 tower computer running Windows® XP SP3. Note that the instrument does not require a computer to start a run. Any other computer configurations are not fully tested.

Answer Id: E2328

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a85e6c43593177fa4c71ed35083de301_FAQ

How do I know if my software license has expired?

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Answer

You can check your license status by opening the instrument software and going to Tools --> License Central. You will see the license status and the expiration date.

Answer Id: E6626

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480a67da8b91a036776fb68dfd8280d3_FAQ

How do I renew my software license?

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Answer

User Bulletin v1.2.1 has specific directions for how to renew your software license. Please search the term '4474347' on our website. The renewal directions apply to the ViiA™ 7, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex systems.

Answer Id: E6627

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791a8b8d5a74c55e1320b96214dc92b8_FAQ

What real-time instruments can be run independently from a computer?

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Answer

The StepOne™, StepOnePlus™, ViiA™ 7, and QuantStudio™ 12K Flex systems can all be run directly from the instrument touchscreen and don't need a separate computer to operate them.

Answer Id: E6303

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758c0344354c6150c72f9b3318315d75_FAQ

What dyes can I use with the ViiA™ 7 Real-Time PCR System?

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Answer

The ViiA™ 7 is calibrated for the following dyes: FAM™, VIC™, ROX®, SYBR®, NED, and TAMRA. In addition, it can be used with custom dyes that excite between 455 and 672 nm and read between 505 and 723 nm.

Answer Id: E6305

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a4ed84dd1504ddaa200433829afb0891_FAQ

Can I import a standard curve for my real-time data?

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Answer

It is possible to import a standard curve if you have either the ViiA™ 7 or QuantStudio™ 12K Flex systems. In the ViiA™ 7 or QuantStudio&trade instrument software, open the data file and go to 'Analysis Settings'. Click on the 'Standard Curve Settings' tab. You will see an option to 'Use a standard curve imported from another experiment'. Choose this, then click on "Import". Browse to the proper data file and click "Select".

Answer Id: E6310

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919d746817b295356ea751c29400d2de_FAQ

How many files can I store on the ViiA™ 7 system?

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Answer

The ViiA™ 7 can store up to 100 data files. When the instrument data storage becomes full, simply touch the 'Collect Results' button and select the files you want to delete to free up more space.

Answer Id: E6312

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1086d76705012c190a3ba1db40ad554c_FAQ

How do I set up a custom dye calibration in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

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Answer

Follow the directions on page 10 of this User Bulletin to add a custom dye to your ViiA™ 7 or QuantStudio™ 12K Flex system.

Answer Id: E6317

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13c4e8ddec4ae95a56f68c74ec04fa16_FAQ

What is the normalization calibration for in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

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Answer

The normalization calibration generates factors that the software uses when comparing data from multiple instruments.

Answer Id: E6318

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51ef07cca8f42f10f47f72f199a4a97a_FAQ

What should I do if I need to transfer my ViiA™ 7 system software license to another computer?

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Answer

If you need to move your ViiA™ 7 system software to another computer, please contact instrumentservices@lifetech.com with your instrument serial number.

Answer Id: E6319

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d2f1457085826347135db95b3d365894_FAQ

What concentration of ROX® should I use for the ViiA™ 7 system?

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Answer

If using a mastermix in which ROX® dye is provided in a separate tube, please add it to 50 nM (in the final reaction well).

Answer Id: E6320

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f903d6c3cc4014d249141c5eb1dc9103_FAQ

Can I see the amplifcation plot on the instrument touchscreen?

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Answer

Yes, on the QuantStudio 12K Flex system you can see the real time amplification plots on the touchscreen. On other systems, you will only be able to see the amplification plots in real-time on the connected computer.

Answer Id: E6321

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8493869fc9c36f237ea5b33e7f1ee8e7_FAQ

When do I need to calibrate the ViiA™ 7 Real-Time PCR System?

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Answer

The recommended maintenance schedule and calibration instructions can be found in the User Guide here: http://tools.lifetechnologies.com/content/sfs/manuals/4442661C.pdf
In brief, the background calibration is recommended to be done every month. All other calibrations (pure dye, ROI, uniformity, and normalization) are recommended to be done every six months.

Answer Id: E7183

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a89654c2b2ee0245f095c546cf9fbddb_FAQ

What are the uniformity and normalization calibrations for on the ViiA™ 7 Real-Time PCR System?

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Answer

The uniformity calibration generates data that allow the software to compensate for the physical effects of the ViiA™ 7 system filters using the ROI plate. The normalization calibration generates factors that the software uses when comparing data from multiple ViiA™ 7 instruments within a study. Two normalization plates are provided in the calibration kit, one containing FAM™/ROX™ dyes and the other with VIC®/ROX™ dyes.

Answer Id: E7184

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791370dbdc977653643ee676fd27808f_FAQ