Can any of the Applied Biosystems® Real-Time PCR Systems detect one copy?
All Applied Biosystems® instrument systems are qualified to detect a two-fold change. Scientifically speaking, there is a statistical calculation that predicts the probability of detecting one molecule in any given sample called the Poisson Distribution. The Poisson Distribution predicts that one molecule will be detected 66% of the time. This is assuming that the assay has been optimized to the fullest capacity and that there are no aliquoting or pipetting errors involved.
Please refer to the following paper concerning one-copy detection. Lockey, C., E. Otto, and Z. Long. "Real-time fluorescence detection of a single DNA molecule." BioTechniques 24 (1998): 744-746.
Each assay designed on one of our platforms needs to be optimized to determine the Linear Dynamic Range of the assay. An Applied Biosystems® Sequence Detection System (Real-Time PCR instrument) may be able to detect one copy in a fully optimized assay. As stated above, one copy can only be detected 66% of the time in a fully optimized system.
Answer Id: 1824
What computer configurations can I use with the ViiA™ 7 Fast Real-Time PCR System?
Can I use a custom dye on my Applied Biosystems® 7500, 7500 Fast, 7900HT Fast or other Real-Time PCR System?
Yes, you can run custom dyes, but the custom dye must fall into the wavelengths that are capable of being detected by a specific instrument. For optimal results on the filter-based instruments (including 7500 and 7500 Fast), the custom dye emission wavelength must fall into one of the emission filter peak detection wavelengths. For the laser-based 7900HT systems, the custom dye wavelength must fall within the overall range of detection for the instrument. Please refer to the Real-Time PCR information pages on our website under Products & Services for profiles of each instrument with a list of compatible standard dyes and the corresponding wavelengths supported. Please also refer to your User Manual or Installation and Maintenance Manual for instructions on how to calibrate custom dyes on your particular instrument.
Answer Id: 2457
What are some of the main differences between Applied Biosystems® Real-Time PCR Systems, like the 7500, 7900HT, 7700 or 7000 Sequence Detection System instruments?
The systems most often vary by the light source and filter sets. The 7000, 7300, 7500, and 7500 Fast systems utilize a halogen lamp as the excitation source and emission filters to detect the emission fluorescence. The 7000 and 7300 systems both have one excitation and four emission filters, while the 7500 and 7500 Fast systems have five excitation and five emission filters. Five excitation and five emission filters allow the 7500 and 7500 Fast systems to detect dyes in the far red range. The 7700 and 7900HT systems utilize an argon ion laser as the excitation source and the instrument scans across all wavelengths between 520 nm to 610 nm.
The 7500, 7500 Fast and 7900HT systems also offers the ability to upgrade to the Fast chemistry; typical real-time runs take about 30-45 minutes.
In addition, the 7900HT system offers further high throughput options such as a 384-well block and a TaqMan® Low Density Array Upgrade as well as a Zymark Twister Automation Accessory that will robotically load plates on the instrument.
For further information please speak with your local Life Technologies sales representative.
Answer Id: 2459
What are the extensions of files generated by the ABI PRISM® 7000 Sequence Detection System, Applied Biosystems® 7300, 7500, 7500Fast, 7900HT Fast Real-Time PCR System, StepOnePlus™ Real-Time PCR System, and ViiA™ 7 Real-Time PCR Systems?
All run files have an .SDS or .EDS extension. A Relative Quantification study will have an .SDM or .EDM extension and a template will have an .SDT or .EDT extension. The software for StepOnePlus™, ViiA™ 7, and 7500 2.0.x software have .EDS, .EDT, or .EDM extensions; all other software have .SDS, .SDT, or .SDM extensions.
Answer Id: 2473
How do I know if my software license has expired?
How do I renew my software license?
User Bulletin v1.2.1 has specific directions for how to renew your software license. Please search the term '4474347' on our website. The renewal directions apply to the ViiA™ 7, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex systems.
Answer Id: 6627
What real-time instruments can be run independently from a computer?
What dyes can I use with the ViiA™ 7 Real-Time PCR System?
Can I import a standard curve for my real-time data?
It is possible to import a standard curve if you have either the ViiA™ 7 or QuantStudio™ 12K Flex systems. In the ViiA™ 7 or QuantStudio&trade instrument software, open the data file and go to 'Analysis Settings'. Click on the 'Standard Curve Settings' tab. You will see an option to 'Use a standard curve imported from another experiment'. Choose this, then click on "Import". Browse to the proper data file and click "Select".
Answer Id: 6310
How many files can I store on the ViiA™ 7 system?
How do I set up a custom dye calibration in the ViiA™ 7 or QuantStudio™ 12K Flex systems?
What is the normalization calibration for in the ViiA™ 7 or QuantStudio™ 12K Flex systems?
What should I do if I need to transfer my ViiA™ 7 system software license to another computer?
What concentration of ROX® should I use for the ViiA™ 7 system?