What are the extensions of files generated by the ABI PRISM® 7000 Sequence Detection System, Applied Biosystems® 7300, 7500, 7500Fast, 7900HT Fast Real-Time PCR System, StepOnePlus™ Real-Time PCR System, and ViiA™ 7 Real-Time PCR Systems?

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All run files have an .SDS or .EDS extension. A Relative Quantification study will have an .SDM or .EDM extension and a template will have an .SDT or .EDT extension. The software for StepOnePlus™, ViiA™ 7, and 7500 2.0.x software have .EDS, .EDT, or .EDM extensions; all other software have .SDS, .SDT, or .SDM extensions.

Answer Id: 2473

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5e6bd7a6970cd4325e587f02667f7f73_FAQ

What computer configurations can I use with the ViiA™ 7 Fast Real-Time PCR System?

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The current recommended configuration is the Dell Gx980 tower computer running Windows® XP SP3. Note that the instrument does not require a computer to start a run. Any other computer configurations are not fully tested.

Answer Id: 2328

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fd69dbe29f156a7ef876a40a94f65599_FAQ

Can I use a custom dye on my Applied Biosystems® 7500, 7500 Fast, 7900HT Fast or other Real-Time PCR System?

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Yes, you can run custom dyes, but the custom dye must fall into the wavelengths that are capable of being detected by a specific instrument. For optimal results on the filter-based instruments (including 7500 and 7500 Fast), the custom dye emission wavelength must fall into one of the emission filter peak detection wavelengths. For the laser-based 7900HT systems, the custom dye wavelength must fall within the overall range of detection for the instrument. Please refer to the Real-Time PCR information pages on our website under “Products & Services” for profiles of each instrument with a list of compatible standard dyes and the corresponding wavelengths supported. Please also refer to your User Manual or Installation and Maintenance Manual for instructions on how to calibrate custom dyes on your particular instrument.

Answer Id: 2457

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7e05d6f828574fbc975a896b25bb011e_FAQ

What are some of the main differences between Applied Biosystems® Real-Time PCR Systems, like the 7500, 7900HT, 7700 or 7000 Sequence Detection System instruments?

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The systems most often vary by the light source and filter sets. The 7000, 7300, 7500, and 7500 Fast systems utilize a halogen lamp as the excitation source and emission filters to detect the emission fluorescence. The 7000 and 7300 systems both have one excitation and four emission filters, while the 7500 and 7500 Fast systems have five excitation and five emission filters. Five excitation and five emission filters allow the 7500 and 7500 Fast systems to detect dyes in the far red range. The 7700 and 7900HT systems utilize an argon ion laser as the excitation source and the instrument scans across all wavelengths between 520 nm to 610 nm.

The 7500, 7500 Fast and 7900HT systems also offers the ability to upgrade to the Fast chemistry; typical real-time runs take about 30-45 minutes.

In addition, the 7900HT system offers further high throughput options such as a 384-well block and a TaqMan® Low Density Array Upgrade as well as a Zymark Twister Automation Accessory that will robotically load plates on the instrument.

For further information please speak with your local Life Technologies sales representative.

Answer Id: 2459

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8bdb5058376143fa358981954e7626b8_FAQ

Can any of the Applied Biosystems® Real-Time PCR Systems detect one copy?

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All Applied Biosystems® instrument systems are qualified to detect a two-fold change. Scientifically speaking, there is a statistical calculation that predicts the probability of detecting one molecule in any given sample called the Poisson Distribution. The Poisson Distribution predicts that one molecule will be detected 66% of the time. This is assuming that the assay has been optimized to the fullest capacity and that there are no aliquoting or pipetting errors involved.

Please refer to the following paper concerning one-copy detection. Lockey, C., E. Otto, and Z. Long. "Real-time fluorescence detection of a single DNA molecule." BioTechniques 24 (1998): 744-746.

Each assay designed on one of our platforms needs to be optimized to determine the Linear Dynamic Range of the assay. An Applied Biosystems® Sequence Detection System (Real-Time PCR instrument) may be able to detect one copy in a fully optimized assay. As stated above, one copy can only be detected 66% of the time in a fully optimized system.

Answer Id: 1824

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2647c1dba23bc0e0f9cdf75339e120d2_FAQ

How do I know if my software license has expired?

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You can check your license status by opening the instrument software and going to Tools --> License Central. You will see the license status and the expiration date.

Answer Id: 6626

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3483e5ec0489e5c394b028ec4e81f3e1_FAQ

How do I renew my software license?

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User Bulletin v1.2.1 has specific directions for how to renew your software license. Please search the term '4474347' on our website. The renewal directions apply to the ViiA™ 7, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex systems.

Answer Id: 6627

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0224cd598e48c5041c7947fd5cb20d53_FAQ

What real-time instruments can be run independently from a computer?

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The StepOne™, StepOnePlus™, ViiA™ 7, and QuantStudio™ 12K Flex systems can all be run directly from the instrument touchscreen and don't need a separate computer to operate them.

Answer Id: 6303

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141661fa46b11782745bb974d5140004_FAQ

What dyes can I use with the ViiA™ 7 Real-Time PCR System?

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The ViiA™ 7 is calibrated for the following dyes: FAM™, VIC™, ROX®, SYBR®, NED, and TAMRA. In addition, it can be used with custom dyes that excite between 455 and 672 nm and read between 505 and 723 nm.

Answer Id: 6305

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90e69a6d2ad189b222ac1998abe63aea_FAQ

Can I import a standard curve for my real-time data?

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It is possible to import a standard curve if you have either the ViiA™ 7 or QuantStudio™ 12K Flex systems. In the ViiA™ 7 or QuantStudio&trade instrument software, open the data file and go to 'Analysis Settings'. Click on the 'Standard Curve Settings' tab. You will see an option to 'Use a standard curve imported from another experiment'. Choose this, then click on "Import". Browse to the proper data file and click "Select".

Answer Id: 6310

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9381fc93ad66f9ec4b2eef71147a6665_FAQ

How many files can I store on the ViiA™ 7 system?

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The ViiA™ 7 can store up to 100 data files. When the instrument data storage becomes full, simply touch the 'Collect Results' button and select the files you want to delete to free up more space.

Answer Id: 6312

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6467c327eaf8940b4dd07a08c63c5e85_FAQ

How do I set up a custom dye calibration in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

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Follow the directions on page 10 of this User Bulletin to add a custom dye to your ViiA™ 7 or QuantStudio™ 12K Flex system.

Answer Id: 6317

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0e9b734aa25ca8096cb7b56dc0dd8929_FAQ

What is the normalization calibration for in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

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The normalization calibration generates factors that the software uses when comparing data from multiple instruments.

Answer Id: 6318

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b0eb9a95e8b085e4025eae2f0d76a6a6_FAQ

What should I do if I need to transfer my ViiA™ 7 system software license to another computer?

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If you need to move your ViiA™ 7 system software to another computer, please contact instrumentservices@lifetech.com with your instrument serial number.

Answer Id: 6319

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96f2d6069db8ad895c34e2285d25c0ed_FAQ

What concentration of ROX® should I use for the ViiA™ 7 system?

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If using a mastermix in which ROX® dye is provided in a separate tube, please add it to 50 nM (in the final reaction well).

Answer Id: 6320

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