What computer configurations can I use with the ViiA™ 7 Fast Real-Time PCR System?

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The current recommended configuration is the Dell Gx980 tower computer running Windows® XP SP3. Note that the instrument does not require a computer to start a run. Any other computer configurations are not fully tested.

Answer Id: 2328

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fd69dbe29f156a7ef876a40a94f65599_FAQ

How do I know if my software license has expired?

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You can check your license status by opening the instrument software and going to Tools --> License Central. You will see the license status and the expiration date.

Answer Id: 6626

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How do I renew my software license?

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User Bulletin v1.2.1 has specific directions for how to renew your software license. Please search the term '4474347' on our website. The renewal directions apply to the ViiA™ 7, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex systems.

Answer Id: 6627

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0224cd598e48c5041c7947fd5cb20d53_FAQ

What real-time instruments can be run independently from a computer?

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The StepOne™, StepOnePlus™, ViiA™ 7, and QuantStudio™ 12K Flex systems can all be run directly from the instrument touchscreen and don't need a separate computer to operate them.

Answer Id: 6303

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What dyes can I use with the ViiA™ 7 Real-Time PCR System?

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The ViiA™ 7 is calibrated for the following dyes: FAM™, VIC™, ROX®, SYBR®, NED, and TAMRA. In addition, it can be used with custom dyes that excite between 455 and 672 nm and read between 505 and 723 nm.

Answer Id: 6305

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Can I import a standard curve for my real-time data?

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It is possible to import a standard curve if you have either the ViiA™ 7 or QuantStudio™ 12K Flex systems. In the ViiA™ 7 or QuantStudio&trade instrument software, open the data file and go to 'Analysis Settings'. Click on the 'Standard Curve Settings' tab. You will see an option to 'Use a standard curve imported from another experiment'. Choose this, then click on "Import". Browse to the proper data file and click "Select".

Answer Id: 6310

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9381fc93ad66f9ec4b2eef71147a6665_FAQ

How many files can I store on the ViiA™ 7 system?

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The ViiA™ 7 can store up to 100 data files. When the instrument data storage becomes full, simply touch the 'Collect Results' button and select the files you want to delete to free up more space.

Answer Id: 6312

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6467c327eaf8940b4dd07a08c63c5e85_FAQ

How do I set up a custom dye calibration in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

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Follow the directions on page 10 of this User Bulletin to add a custom dye to your ViiA™ 7 or QuantStudio™ 12K Flex system.

Answer Id: 6317

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What is the normalization calibration for in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

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The normalization calibration generates factors that the software uses when comparing data from multiple instruments.

Answer Id: 6318

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What should I do if I need to transfer my ViiA™ 7 system software license to another computer?

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If you need to move your ViiA™ 7 system software to another computer, please contact instrumentservices@lifetech.com with your instrument serial number.

Answer Id: 6319

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What concentration of ROX® should I use for the ViiA™ 7 system?

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If using a mastermix in which ROX® dye is provided in a separate tube, please add it to 50 nM (in the final reaction well).

Answer Id: 6320

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Can I see the amplifcation plot on the instrument touchscreen?

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Yes, on the QuantStudio 12K Flex system you can see the real time amplification plots on the touchscreen. On other systems, you will only be able to see the amplification plots in real-time on the connected computer.

Answer Id: 6321

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When do I need to calibrate the ViiA™ 7 Real-Time PCR System?

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The recommended maintenance schedule and calibration instructions can be found in the User Guide here: http://tools.lifetechnologies.com/content/sfs/manuals/4442661C.pdf
In brief, the background calibration is recommended to be done every month. All other calibrations (pure dye, ROI, uniformity, and normalization) are recommended to be done every six months.

Answer Id: 7183

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607bc9ebe4abfcd65181bfbef6252830_FAQ

What are the uniformity and normalization calibrations for on the ViiA™ 7 Real-Time PCR System?

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The uniformity calibration generates data that allow the software to compensate for the physical effects of the ViiA™ 7 system filters using the ROI plate. The normalization calibration generates factors that the software uses when comparing data from multiple ViiA™ 7 instruments within a study. Two normalization plates are provided in the calibration kit, one containing FAM™/ROX™ dyes and the other with VIC®/ROX™ dyes.

Answer Id: 7184

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0e674a918ebca3f78bfe02e2f387689d_FAQ

Can I reuse my calibration plates?

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The calibration plates can be stored and reused three times for up to 1 year after you first open them, so make sure to return them to their original packaging and return them to -20 degrees C storage until the next use. (Note: Due to the small volumes, calibration array cards cannot be reused. Please discard them after use.) If needed, you can make your own background plate using deionized water. Please follow the directions in the ViiA™ 7 Real-Time PCR System Installation, Networking, and Maintenance Guide for more details at http://tools.lifetechnologies.com/content/sfs/manuals/4442661C.pdf.

Answer Id: 7185

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