How does siPORT™ Amine transfection agent differ from siPORT™ NeoFX™ transfection agent?

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Answer

These two transfection agents vary by chemistry.  siPORT™ NeoFX™ transfection agent is a liposomal agent, while siPORT™ Amine transfection agent possesses polyamine chemistry.

Answer Id: E2886

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4fb48adead3bf66b567b0ca6d58696e7_FAQ

Can siPORT™ Amine transfection agent  be used for co-transfection with an expression vector?

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Answer

Yes; siRNAs can be co-transfected with plasmids using siPORT™ Amine transfection agent.  The amount of plasmid and siRNA will need to be optimized by the researcher.

Answer Id: E2887

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3915e70f8f5653e3744d72ef7602a889_FAQ

Can reverse transfection be performed with siPORT™ Amine transfection agent?

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Answer

Yes.  However, with this reagent our scientists found that many cell types will be transfected with higher efficiency using the traditional "forward" pre-plating protocol instead.

Answer Id: E2888

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