Is the LMG194 sold by Life Technologies the same as that in the ATCC bank?

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Answer

Yes, it is the same strain. ATCC Number: 47090

Answer Id: E3246

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3048aa7a49c7ed43ab01e7652003f954_FAQ

What protein yields should I expect to get with the pRSET expression vectors?

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Answer

We've expressed CAT and Beta-Gal in volumes from 2 ml to 50 ml and get about 20-50 µg protein/ml of culture. Protein yield is dependent on the type of protein being expressed and the culturing conditions.

Answer Id: E3259

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6d668e5b96b629314469c673ebbc33f1_FAQ

Are NP40 and DTT required for SUMO™ Protease activity? Will they interfere with downstream applications?

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Answer

Yes, they are both required, although it might be possible to substitute the NP40 with another detergent via dialysis if you have concern about interference with mass spectrometry. DTT is an absolute requirement.

Answer Id: E4965

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